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Fatty Acids of CLA-Enriched Egg Yolks Can Induce Transcriptional Activation of Peroxisome Proliferator-Activated Receptors in MCF-7 Breast Cancer Cells

In our previous study, we showed that fatty acids from CLA-enriched egg yolks (EFA-CLA) reduced the proliferation of breast cancer cells; however, the molecular mechanisms of their action remain unknown. In the current study, we used MCF-7 breast cancer cell line to determine the effect of EFA-CLA,...

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Autores principales: Koronowicz, Aneta A., Banks, Paula, Master, Adam, Domagała, Dominik, Piasna-Słupecka, Ewelina, Drozdowska, Mariola, Sikora, Elżbieta, Laidler, Piotr
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Hindawi 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5385215/
https://www.ncbi.nlm.nih.gov/pubmed/28458685
http://dx.doi.org/10.1155/2017/2865283
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author Koronowicz, Aneta A.
Banks, Paula
Master, Adam
Domagała, Dominik
Piasna-Słupecka, Ewelina
Drozdowska, Mariola
Sikora, Elżbieta
Laidler, Piotr
author_facet Koronowicz, Aneta A.
Banks, Paula
Master, Adam
Domagała, Dominik
Piasna-Słupecka, Ewelina
Drozdowska, Mariola
Sikora, Elżbieta
Laidler, Piotr
author_sort Koronowicz, Aneta A.
collection PubMed
description In our previous study, we showed that fatty acids from CLA-enriched egg yolks (EFA-CLA) reduced the proliferation of breast cancer cells; however, the molecular mechanisms of their action remain unknown. In the current study, we used MCF-7 breast cancer cell line to determine the effect of EFA-CLA, as potential ligands for peroxisome proliferator-activated receptors (PPARs), on identified in silico PPAR-responsive genes: BCAR3, TCF20, WT1, ZNF621, and THRB (transcript TRβ2). Our results showed that EFA-CLA act as PPAR ligands with agonistic activity for all PPAR isoforms, with the highest specificity towards PPARγ. In conclusion, we propose that EFA-CLA-mediated regulation of PPAR-responsive genes is most likely facilitated by cis9,trans11CLA isomer incorporated in egg yolk. Notably, EFA-CLA activated PPAR more efficiently than nonenriched FA as well as synthetic CLA isomers. We also propose that this regulation, at least in part, can be responsible for the observed reduction in the proliferation of MCF-7 cells treated with EFA-CLA.
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spelling pubmed-53852152017-04-30 Fatty Acids of CLA-Enriched Egg Yolks Can Induce Transcriptional Activation of Peroxisome Proliferator-Activated Receptors in MCF-7 Breast Cancer Cells Koronowicz, Aneta A. Banks, Paula Master, Adam Domagała, Dominik Piasna-Słupecka, Ewelina Drozdowska, Mariola Sikora, Elżbieta Laidler, Piotr PPAR Res Research Article In our previous study, we showed that fatty acids from CLA-enriched egg yolks (EFA-CLA) reduced the proliferation of breast cancer cells; however, the molecular mechanisms of their action remain unknown. In the current study, we used MCF-7 breast cancer cell line to determine the effect of EFA-CLA, as potential ligands for peroxisome proliferator-activated receptors (PPARs), on identified in silico PPAR-responsive genes: BCAR3, TCF20, WT1, ZNF621, and THRB (transcript TRβ2). Our results showed that EFA-CLA act as PPAR ligands with agonistic activity for all PPAR isoforms, with the highest specificity towards PPARγ. In conclusion, we propose that EFA-CLA-mediated regulation of PPAR-responsive genes is most likely facilitated by cis9,trans11CLA isomer incorporated in egg yolk. Notably, EFA-CLA activated PPAR more efficiently than nonenriched FA as well as synthetic CLA isomers. We also propose that this regulation, at least in part, can be responsible for the observed reduction in the proliferation of MCF-7 cells treated with EFA-CLA. Hindawi 2017 2017-03-26 /pmc/articles/PMC5385215/ /pubmed/28458685 http://dx.doi.org/10.1155/2017/2865283 Text en Copyright © 2017 Aneta A. Koronowicz et al. https://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Koronowicz, Aneta A.
Banks, Paula
Master, Adam
Domagała, Dominik
Piasna-Słupecka, Ewelina
Drozdowska, Mariola
Sikora, Elżbieta
Laidler, Piotr
Fatty Acids of CLA-Enriched Egg Yolks Can Induce Transcriptional Activation of Peroxisome Proliferator-Activated Receptors in MCF-7 Breast Cancer Cells
title Fatty Acids of CLA-Enriched Egg Yolks Can Induce Transcriptional Activation of Peroxisome Proliferator-Activated Receptors in MCF-7 Breast Cancer Cells
title_full Fatty Acids of CLA-Enriched Egg Yolks Can Induce Transcriptional Activation of Peroxisome Proliferator-Activated Receptors in MCF-7 Breast Cancer Cells
title_fullStr Fatty Acids of CLA-Enriched Egg Yolks Can Induce Transcriptional Activation of Peroxisome Proliferator-Activated Receptors in MCF-7 Breast Cancer Cells
title_full_unstemmed Fatty Acids of CLA-Enriched Egg Yolks Can Induce Transcriptional Activation of Peroxisome Proliferator-Activated Receptors in MCF-7 Breast Cancer Cells
title_short Fatty Acids of CLA-Enriched Egg Yolks Can Induce Transcriptional Activation of Peroxisome Proliferator-Activated Receptors in MCF-7 Breast Cancer Cells
title_sort fatty acids of cla-enriched egg yolks can induce transcriptional activation of peroxisome proliferator-activated receptors in mcf-7 breast cancer cells
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5385215/
https://www.ncbi.nlm.nih.gov/pubmed/28458685
http://dx.doi.org/10.1155/2017/2865283
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