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Genetic dissection of seed oil and protein content and identification of networks associated with oil content in Brassica napus
High-density linkage maps can improve the precision of QTL localization. A high-density SNP-based linkage map containing 3207 markers covering 3072.7 cM of the Brassica napus genome was constructed in the KenC-8 × N53-2 (KNDH) population. A total of 67 and 38 QTLs for seed oil and protein content we...
Autores principales: | , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Nature Publishing Group
2017
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5385559/ https://www.ncbi.nlm.nih.gov/pubmed/28393910 http://dx.doi.org/10.1038/srep46295 |
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author | Chao, Hongbo Wang, Hao Wang, Xiaodong Guo, Liangxing Gu, Jianwei Zhao, Weiguo Li, Baojun Chen, Dengyan Raboanatahiry, Nadia Li, Maoteng |
author_facet | Chao, Hongbo Wang, Hao Wang, Xiaodong Guo, Liangxing Gu, Jianwei Zhao, Weiguo Li, Baojun Chen, Dengyan Raboanatahiry, Nadia Li, Maoteng |
author_sort | Chao, Hongbo |
collection | PubMed |
description | High-density linkage maps can improve the precision of QTL localization. A high-density SNP-based linkage map containing 3207 markers covering 3072.7 cM of the Brassica napus genome was constructed in the KenC-8 × N53-2 (KNDH) population. A total of 67 and 38 QTLs for seed oil and protein content were identified with an average confidence interval of 5.26 and 4.38 cM, which could explain up to 22.24% and 27.48% of the phenotypic variation, respectively. Thirty-eight associated genomic regions from BSA overlapped with and/or narrowed the SOC-QTLs, further confirming the QTL mapping results based on the high-density linkage map. Potential candidates related to acyl-lipid and seed storage underlying SOC and SPC, respectively, were identified and analyzed, among which six were checked and showed expression differences between the two parents during different embryonic developmental periods. A large primary carbohydrate pathway based on potential candidates underlying SOC- and SPC-QTLs, and interaction networks based on potential candidates underlying SOC-QTLs, was constructed to dissect the complex mechanism based on metabolic and gene regulatory features, respectively. Accurate QTL mapping and potential candidates identified based on high-density linkage map and BSA analyses provide new insights into the complex genetic mechanism of oil and protein accumulation in the seeds of rapeseed. |
format | Online Article Text |
id | pubmed-5385559 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2017 |
publisher | Nature Publishing Group |
record_format | MEDLINE/PubMed |
spelling | pubmed-53855592017-04-12 Genetic dissection of seed oil and protein content and identification of networks associated with oil content in Brassica napus Chao, Hongbo Wang, Hao Wang, Xiaodong Guo, Liangxing Gu, Jianwei Zhao, Weiguo Li, Baojun Chen, Dengyan Raboanatahiry, Nadia Li, Maoteng Sci Rep Article High-density linkage maps can improve the precision of QTL localization. A high-density SNP-based linkage map containing 3207 markers covering 3072.7 cM of the Brassica napus genome was constructed in the KenC-8 × N53-2 (KNDH) population. A total of 67 and 38 QTLs for seed oil and protein content were identified with an average confidence interval of 5.26 and 4.38 cM, which could explain up to 22.24% and 27.48% of the phenotypic variation, respectively. Thirty-eight associated genomic regions from BSA overlapped with and/or narrowed the SOC-QTLs, further confirming the QTL mapping results based on the high-density linkage map. Potential candidates related to acyl-lipid and seed storage underlying SOC and SPC, respectively, were identified and analyzed, among which six were checked and showed expression differences between the two parents during different embryonic developmental periods. A large primary carbohydrate pathway based on potential candidates underlying SOC- and SPC-QTLs, and interaction networks based on potential candidates underlying SOC-QTLs, was constructed to dissect the complex mechanism based on metabolic and gene regulatory features, respectively. Accurate QTL mapping and potential candidates identified based on high-density linkage map and BSA analyses provide new insights into the complex genetic mechanism of oil and protein accumulation in the seeds of rapeseed. Nature Publishing Group 2017-04-10 /pmc/articles/PMC5385559/ /pubmed/28393910 http://dx.doi.org/10.1038/srep46295 Text en Copyright © 2017, The Author(s) http://creativecommons.org/licenses/by/4.0/ This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/ |
spellingShingle | Article Chao, Hongbo Wang, Hao Wang, Xiaodong Guo, Liangxing Gu, Jianwei Zhao, Weiguo Li, Baojun Chen, Dengyan Raboanatahiry, Nadia Li, Maoteng Genetic dissection of seed oil and protein content and identification of networks associated with oil content in Brassica napus |
title | Genetic dissection of seed oil and protein content and identification of networks associated with oil content in Brassica napus |
title_full | Genetic dissection of seed oil and protein content and identification of networks associated with oil content in Brassica napus |
title_fullStr | Genetic dissection of seed oil and protein content and identification of networks associated with oil content in Brassica napus |
title_full_unstemmed | Genetic dissection of seed oil and protein content and identification of networks associated with oil content in Brassica napus |
title_short | Genetic dissection of seed oil and protein content and identification of networks associated with oil content in Brassica napus |
title_sort | genetic dissection of seed oil and protein content and identification of networks associated with oil content in brassica napus |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5385559/ https://www.ncbi.nlm.nih.gov/pubmed/28393910 http://dx.doi.org/10.1038/srep46295 |
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