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Effects of Humanin on Experimental Colitis Induced by 2,4,6-trinitrobenzene Sulphonic Acid in Rats
BACKGROUND/AIM: The excessive apoptosis of intestinal epithelial cells (IECs) partly accounts for the development of colonic inflammation and eventually results in ulcerative colitis (UC). Humanin, an endogenous anti-apoptotic peptide, has previously been shown to protect against Alzheimer's di...
Autores principales: | , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Medknow Publications & Media Pvt Ltd
2017
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5385712/ https://www.ncbi.nlm.nih.gov/pubmed/28361841 http://dx.doi.org/10.4103/sjg.SJG_318_16 |
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author | Gultekin, Fatma A. Emre, Ali U. Celik, Sevim K. Barut, Figen Tali, Ufuk Sumer, Demet Turkcu, Ummuhani O. |
author_facet | Gultekin, Fatma A. Emre, Ali U. Celik, Sevim K. Barut, Figen Tali, Ufuk Sumer, Demet Turkcu, Ummuhani O. |
author_sort | Gultekin, Fatma A. |
collection | PubMed |
description | BACKGROUND/AIM: The excessive apoptosis of intestinal epithelial cells (IECs) partly accounts for the development of colonic inflammation and eventually results in ulcerative colitis (UC). Humanin, an endogenous anti-apoptotic peptide, has previously been shown to protect against Alzheimer's disease and a variety of cellular insults. The present study aimed to investigate the effects of glysin variant of humanin (HNG) on 2,4,6-trinitrobenzene sulphonic acid (TNBS)-induced colitis in rats. MATERIALS AND METHODS: Rats were divided into four groups as follows: Group 1 (n = 8): control; isotonic saline solution 0.1 ml/rat rectally, Group 2 (n = 8): TNBS colitis; 0.1 ml of a 2.5% (w/v) TNBS solution in 50% ethanol rectally, Group 3 (n = 8): 10 μM HNG, and Group 4 (n = 8): 20 μM HNG intraperitoneal (ip) on day 2 and 6 after rectal TNBS administration. Rats were sacrificed 7 days after the induction of colitis. Blood and tissue samples were harvested for biochemical and histopathological analysis. RESULTS: HNG treatment significantly ameliorated weight loss and macroscopic and microscopic scores. TNBS-induced colitis significantly increased the colonic mRNA expression of tumor necrosis factor-alpha (TNF-α), interleukin-1beta (IL-1β), and caspase-3 activities in group II in comparison to the group I. HNG treatment was associated with an inhibition of mRNA expression of TNF-α and IL-1β, and a decrease in caspase-3 activities in colon tissues in group III and IV when compared to group II. CONCLUSION: The results of this study indicate that HNG treatment may exert beneficial effects in UC by decreasing inflammatory reactions and apoptosis. |
format | Online Article Text |
id | pubmed-5385712 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2017 |
publisher | Medknow Publications & Media Pvt Ltd |
record_format | MEDLINE/PubMed |
spelling | pubmed-53857122017-05-17 Effects of Humanin on Experimental Colitis Induced by 2,4,6-trinitrobenzene Sulphonic Acid in Rats Gultekin, Fatma A. Emre, Ali U. Celik, Sevim K. Barut, Figen Tali, Ufuk Sumer, Demet Turkcu, Ummuhani O. Saudi J Gastroenterol Original Article BACKGROUND/AIM: The excessive apoptosis of intestinal epithelial cells (IECs) partly accounts for the development of colonic inflammation and eventually results in ulcerative colitis (UC). Humanin, an endogenous anti-apoptotic peptide, has previously been shown to protect against Alzheimer's disease and a variety of cellular insults. The present study aimed to investigate the effects of glysin variant of humanin (HNG) on 2,4,6-trinitrobenzene sulphonic acid (TNBS)-induced colitis in rats. MATERIALS AND METHODS: Rats were divided into four groups as follows: Group 1 (n = 8): control; isotonic saline solution 0.1 ml/rat rectally, Group 2 (n = 8): TNBS colitis; 0.1 ml of a 2.5% (w/v) TNBS solution in 50% ethanol rectally, Group 3 (n = 8): 10 μM HNG, and Group 4 (n = 8): 20 μM HNG intraperitoneal (ip) on day 2 and 6 after rectal TNBS administration. Rats were sacrificed 7 days after the induction of colitis. Blood and tissue samples were harvested for biochemical and histopathological analysis. RESULTS: HNG treatment significantly ameliorated weight loss and macroscopic and microscopic scores. TNBS-induced colitis significantly increased the colonic mRNA expression of tumor necrosis factor-alpha (TNF-α), interleukin-1beta (IL-1β), and caspase-3 activities in group II in comparison to the group I. HNG treatment was associated with an inhibition of mRNA expression of TNF-α and IL-1β, and a decrease in caspase-3 activities in colon tissues in group III and IV when compared to group II. CONCLUSION: The results of this study indicate that HNG treatment may exert beneficial effects in UC by decreasing inflammatory reactions and apoptosis. Medknow Publications & Media Pvt Ltd 2017 /pmc/articles/PMC5385712/ /pubmed/28361841 http://dx.doi.org/10.4103/sjg.SJG_318_16 Text en Copyright: © 2017 Saudi Journal of Gastroenterology (Official journal of The Saudi Gastroenterology Association) http://creativecommons.org/licenses/by-nc-sa/3.0 This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-ShareAlike 3.0 License, which allows others to remix, tweak, and build upon the work non-commercially, as long as the author is credited and the new creations are licensed under the identical terms. |
spellingShingle | Original Article Gultekin, Fatma A. Emre, Ali U. Celik, Sevim K. Barut, Figen Tali, Ufuk Sumer, Demet Turkcu, Ummuhani O. Effects of Humanin on Experimental Colitis Induced by 2,4,6-trinitrobenzene Sulphonic Acid in Rats |
title | Effects of Humanin on Experimental Colitis Induced by 2,4,6-trinitrobenzene Sulphonic Acid in Rats |
title_full | Effects of Humanin on Experimental Colitis Induced by 2,4,6-trinitrobenzene Sulphonic Acid in Rats |
title_fullStr | Effects of Humanin on Experimental Colitis Induced by 2,4,6-trinitrobenzene Sulphonic Acid in Rats |
title_full_unstemmed | Effects of Humanin on Experimental Colitis Induced by 2,4,6-trinitrobenzene Sulphonic Acid in Rats |
title_short | Effects of Humanin on Experimental Colitis Induced by 2,4,6-trinitrobenzene Sulphonic Acid in Rats |
title_sort | effects of humanin on experimental colitis induced by 2,4,6-trinitrobenzene sulphonic acid in rats |
topic | Original Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5385712/ https://www.ncbi.nlm.nih.gov/pubmed/28361841 http://dx.doi.org/10.4103/sjg.SJG_318_16 |
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