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Calcium signals drive cell shape changes during zebrafish midbrain–hindbrain boundary formation

One of the first morphogenetic events in the vertebrate brain is the formation of the highly conserved midbrain–hindbrain boundary (MHB). Specific cell shape changes occur at the point of deepest constriction of the MHB, the midbrain–hindbrain boundary constriction (MHBC), and are critical for prope...

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Autores principales: Sahu, Srishti U., Visetsouk, Mike R., Garde, Ryan J., Hennes, Leah, Kwas, Constance, Gutzman, Jennifer H.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: The American Society for Cell Biology 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5385936/
https://www.ncbi.nlm.nih.gov/pubmed/28148652
http://dx.doi.org/10.1091/mbc.E16-08-0561
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author Sahu, Srishti U.
Visetsouk, Mike R.
Garde, Ryan J.
Hennes, Leah
Kwas, Constance
Gutzman, Jennifer H.
author_facet Sahu, Srishti U.
Visetsouk, Mike R.
Garde, Ryan J.
Hennes, Leah
Kwas, Constance
Gutzman, Jennifer H.
author_sort Sahu, Srishti U.
collection PubMed
description One of the first morphogenetic events in the vertebrate brain is the formation of the highly conserved midbrain–hindbrain boundary (MHB). Specific cell shape changes occur at the point of deepest constriction of the MHB, the midbrain–hindbrain boundary constriction (MHBC), and are critical for proper MHB formation. These cell shape changes are controlled by nonmuscle myosin II (NMII) motor proteins, which are tightly regulated via the phosphorylation of their associated myosin regulatory light chains (MRLCs). However, the upstream signaling pathways that initiate the regulation of NMII to mediate cell shape changes during MHB morphogenesis are not known. We show that intracellular calcium signals are critical for the regulation of cell shortening during initial MHB formation. We demonstrate that the MHB region is poised to respond to calcium transients that occur in the MHB at the onset of MHB morphogenesis and that calcium mediates phosphorylation of MRLC specifically in MHB tissue. Our results indicate that calmodulin 1a (calm1a), expressed specifically in the MHB, and myosin light chain kinase together mediate MHBC cell length. Our data suggest that modulation of NMII activity by calcium is critical for proper regulation of cell length to determine embryonic brain shape during development.
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spelling pubmed-53859362017-06-16 Calcium signals drive cell shape changes during zebrafish midbrain–hindbrain boundary formation Sahu, Srishti U. Visetsouk, Mike R. Garde, Ryan J. Hennes, Leah Kwas, Constance Gutzman, Jennifer H. Mol Biol Cell Brief Reports One of the first morphogenetic events in the vertebrate brain is the formation of the highly conserved midbrain–hindbrain boundary (MHB). Specific cell shape changes occur at the point of deepest constriction of the MHB, the midbrain–hindbrain boundary constriction (MHBC), and are critical for proper MHB formation. These cell shape changes are controlled by nonmuscle myosin II (NMII) motor proteins, which are tightly regulated via the phosphorylation of their associated myosin regulatory light chains (MRLCs). However, the upstream signaling pathways that initiate the regulation of NMII to mediate cell shape changes during MHB morphogenesis are not known. We show that intracellular calcium signals are critical for the regulation of cell shortening during initial MHB formation. We demonstrate that the MHB region is poised to respond to calcium transients that occur in the MHB at the onset of MHB morphogenesis and that calcium mediates phosphorylation of MRLC specifically in MHB tissue. Our results indicate that calmodulin 1a (calm1a), expressed specifically in the MHB, and myosin light chain kinase together mediate MHBC cell length. Our data suggest that modulation of NMII activity by calcium is critical for proper regulation of cell length to determine embryonic brain shape during development. The American Society for Cell Biology 2017-04-01 /pmc/articles/PMC5385936/ /pubmed/28148652 http://dx.doi.org/10.1091/mbc.E16-08-0561 Text en © 2017 Sahu, Visetsouk, et al. This article is distributed by The American Society for Cell Biology under license from the author(s). Two months after publication it is available to the public under an Attribution–Noncommercial–Share Alike 3.0 Unported Creative Commons License (http://creativecommons.org/licenses/by-nc-sa/3.0). “ASCB®,” “The American Society for Cell Biology®,” and “Molecular Biology of the Cell®” are registered trademarks of The American Society for Cell Biology.
spellingShingle Brief Reports
Sahu, Srishti U.
Visetsouk, Mike R.
Garde, Ryan J.
Hennes, Leah
Kwas, Constance
Gutzman, Jennifer H.
Calcium signals drive cell shape changes during zebrafish midbrain–hindbrain boundary formation
title Calcium signals drive cell shape changes during zebrafish midbrain–hindbrain boundary formation
title_full Calcium signals drive cell shape changes during zebrafish midbrain–hindbrain boundary formation
title_fullStr Calcium signals drive cell shape changes during zebrafish midbrain–hindbrain boundary formation
title_full_unstemmed Calcium signals drive cell shape changes during zebrafish midbrain–hindbrain boundary formation
title_short Calcium signals drive cell shape changes during zebrafish midbrain–hindbrain boundary formation
title_sort calcium signals drive cell shape changes during zebrafish midbrain–hindbrain boundary formation
topic Brief Reports
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5385936/
https://www.ncbi.nlm.nih.gov/pubmed/28148652
http://dx.doi.org/10.1091/mbc.E16-08-0561
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