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The septin-associated kinase Gin4 recruits Gps1 to the site of cell division

Cell cycle–dependent morphogenesis of unicellular organisms depends on the spatiotemporal control of cell polarity. Rho GTPases are the major players that guide cells through structural reorganizations such as cytokinesis (Rho1 dependent) and polarity establishment (Cdc42 dependent). In budding yeas...

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Autores principales: Meitinger, Franz, Pereira, Gislene
Formato: Online Artículo Texto
Lenguaje:English
Publicado: The American Society for Cell Biology 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5385937/
https://www.ncbi.nlm.nih.gov/pubmed/28148650
http://dx.doi.org/10.1091/mbc.E16-09-0687
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author Meitinger, Franz
Pereira, Gislene
author_facet Meitinger, Franz
Pereira, Gislene
author_sort Meitinger, Franz
collection PubMed
description Cell cycle–dependent morphogenesis of unicellular organisms depends on the spatiotemporal control of cell polarity. Rho GTPases are the major players that guide cells through structural reorganizations such as cytokinesis (Rho1 dependent) and polarity establishment (Cdc42 dependent). In budding yeast, the protein Gps1 plays a pivotal role in both processes. Gps1 resides at the bud neck to maintain Rho1 localization and restrict Cdc42 activity during cytokinesis. Here we analyze how Gps1 is recruited to the bud neck during the cell cycle. We show that different regions of Gps1 and the septin-associated kinase Gin4 are involved in maintaining Gps1 at the bud neck from late G1 phase until midanaphase. From midanaphase, the targeting function of Gin4 is taken over by the bud neck–associated protein Nba1. Our data show that Gps1 is targeted to the cell division site in a biphasic manner, via Gin4 and Nba1, to control the spatiotemporal activation of Rho1 and inhibition of Cdc42.
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spelling pubmed-53859372017-06-16 The septin-associated kinase Gin4 recruits Gps1 to the site of cell division Meitinger, Franz Pereira, Gislene Mol Biol Cell Brief Reports Cell cycle–dependent morphogenesis of unicellular organisms depends on the spatiotemporal control of cell polarity. Rho GTPases are the major players that guide cells through structural reorganizations such as cytokinesis (Rho1 dependent) and polarity establishment (Cdc42 dependent). In budding yeast, the protein Gps1 plays a pivotal role in both processes. Gps1 resides at the bud neck to maintain Rho1 localization and restrict Cdc42 activity during cytokinesis. Here we analyze how Gps1 is recruited to the bud neck during the cell cycle. We show that different regions of Gps1 and the septin-associated kinase Gin4 are involved in maintaining Gps1 at the bud neck from late G1 phase until midanaphase. From midanaphase, the targeting function of Gin4 is taken over by the bud neck–associated protein Nba1. Our data show that Gps1 is targeted to the cell division site in a biphasic manner, via Gin4 and Nba1, to control the spatiotemporal activation of Rho1 and inhibition of Cdc42. The American Society for Cell Biology 2017-04-01 /pmc/articles/PMC5385937/ /pubmed/28148650 http://dx.doi.org/10.1091/mbc.E16-09-0687 Text en © 2017 Meitinger and Pereira. This article is distributed by The American Society for Cell Biology under license from the author(s). Two months after publication it is available to the public under an Attribution–Noncommercial–Share Alike 3.0 Unported Creative Commons License (http://creativecommons.org/licenses/by-nc-sa/3.0). “ASCB®,” “The American Society for Cell Biology®,” and “Molecular Biology of the Cell®” are registered trademarks of The American Society for Cell Biology.
spellingShingle Brief Reports
Meitinger, Franz
Pereira, Gislene
The septin-associated kinase Gin4 recruits Gps1 to the site of cell division
title The septin-associated kinase Gin4 recruits Gps1 to the site of cell division
title_full The septin-associated kinase Gin4 recruits Gps1 to the site of cell division
title_fullStr The septin-associated kinase Gin4 recruits Gps1 to the site of cell division
title_full_unstemmed The septin-associated kinase Gin4 recruits Gps1 to the site of cell division
title_short The septin-associated kinase Gin4 recruits Gps1 to the site of cell division
title_sort septin-associated kinase gin4 recruits gps1 to the site of cell division
topic Brief Reports
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5385937/
https://www.ncbi.nlm.nih.gov/pubmed/28148650
http://dx.doi.org/10.1091/mbc.E16-09-0687
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