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Preventive effect of fermented black ginseng against cisplatin-induced nephrotoxicity in rats
BACKGROUND: Fermented black ginseng (FBG) is processed ginseng by the repeated heat treatment and fermentation of raw ginseng. The protective effect and mechanism of FBG on cisplatin-induced nephrotoxicity was investigated to evaluate its therapeutic potential. METHODS: The free radical scavenging a...
Autores principales: | , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Elsevier
2017
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5386130/ https://www.ncbi.nlm.nih.gov/pubmed/28413323 http://dx.doi.org/10.1016/j.jgr.2016.03.001 |
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author | Jung, Kiwon An, Jun Min Eom, Dae-Woon Kang, Ki Sung Kim, Su-Nam |
author_facet | Jung, Kiwon An, Jun Min Eom, Dae-Woon Kang, Ki Sung Kim, Su-Nam |
author_sort | Jung, Kiwon |
collection | PubMed |
description | BACKGROUND: Fermented black ginseng (FBG) is processed ginseng by the repeated heat treatment and fermentation of raw ginseng. The protective effect and mechanism of FBG on cisplatin-induced nephrotoxicity was investigated to evaluate its therapeutic potential. METHODS: The free radical scavenging activity of FBG was measured using 1,1-diphenyl-2-picrylhydrazyl (DPPH). In addition, the protective effect against cisplatin-induced renal damage was tested in rats. FBG was orally administered every day at a dose of 150 mg/kg body weight for 10 d, and a single dose of cisplatin was administered intraperitoneally (7.5 mg/kg body weight) with 0.9% saline on the 4(th) d. RESULTS: The DPPH radical-scavenging activity of FBG (IC(50) = 384 μg/mL) was stronger than that of raw ginseng. The improved DPPH radical-scavenging activity was mediated by the generation phenolic compounds. The decreased cell viability by cisplatin was recovered significantly after treatment with FBG in a dose-dependent manner. Then, the protective effect of FBG on cisplatin-induced oxidative renal damage was investigated in rats. The decreased creatinine clearance levels, which are a reliable marker for renal dysfunction in cisplatin-treated rats, were reduced to the normal level after the administration of FBG. Moreover, FBG showed protective effects against cisplatin-induced oxidative renal damage in rats through the inhibition of NF-κB/p65, COX-2, and caspase-3 activation. CONCLUSION: These results collectively show that the therapeutic evidence for FBG ameliorates the nephrotoxicity via regulating oxidative stress, inflammation, and apoptosis. |
format | Online Article Text |
id | pubmed-5386130 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2017 |
publisher | Elsevier |
record_format | MEDLINE/PubMed |
spelling | pubmed-53861302017-04-14 Preventive effect of fermented black ginseng against cisplatin-induced nephrotoxicity in rats Jung, Kiwon An, Jun Min Eom, Dae-Woon Kang, Ki Sung Kim, Su-Nam J Ginseng Res Research Article BACKGROUND: Fermented black ginseng (FBG) is processed ginseng by the repeated heat treatment and fermentation of raw ginseng. The protective effect and mechanism of FBG on cisplatin-induced nephrotoxicity was investigated to evaluate its therapeutic potential. METHODS: The free radical scavenging activity of FBG was measured using 1,1-diphenyl-2-picrylhydrazyl (DPPH). In addition, the protective effect against cisplatin-induced renal damage was tested in rats. FBG was orally administered every day at a dose of 150 mg/kg body weight for 10 d, and a single dose of cisplatin was administered intraperitoneally (7.5 mg/kg body weight) with 0.9% saline on the 4(th) d. RESULTS: The DPPH radical-scavenging activity of FBG (IC(50) = 384 μg/mL) was stronger than that of raw ginseng. The improved DPPH radical-scavenging activity was mediated by the generation phenolic compounds. The decreased cell viability by cisplatin was recovered significantly after treatment with FBG in a dose-dependent manner. Then, the protective effect of FBG on cisplatin-induced oxidative renal damage was investigated in rats. The decreased creatinine clearance levels, which are a reliable marker for renal dysfunction in cisplatin-treated rats, were reduced to the normal level after the administration of FBG. Moreover, FBG showed protective effects against cisplatin-induced oxidative renal damage in rats through the inhibition of NF-κB/p65, COX-2, and caspase-3 activation. CONCLUSION: These results collectively show that the therapeutic evidence for FBG ameliorates the nephrotoxicity via regulating oxidative stress, inflammation, and apoptosis. Elsevier 2017-04 2016-03-08 /pmc/articles/PMC5386130/ /pubmed/28413323 http://dx.doi.org/10.1016/j.jgr.2016.03.001 Text en © 2017 The Korean Society of Ginseng, Published by Elsevier Korea LLC. http://creativecommons.org/licenses/by-nc-nd/4.0/ This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/). |
spellingShingle | Research Article Jung, Kiwon An, Jun Min Eom, Dae-Woon Kang, Ki Sung Kim, Su-Nam Preventive effect of fermented black ginseng against cisplatin-induced nephrotoxicity in rats |
title | Preventive effect of fermented black ginseng against cisplatin-induced nephrotoxicity in rats |
title_full | Preventive effect of fermented black ginseng against cisplatin-induced nephrotoxicity in rats |
title_fullStr | Preventive effect of fermented black ginseng against cisplatin-induced nephrotoxicity in rats |
title_full_unstemmed | Preventive effect of fermented black ginseng against cisplatin-induced nephrotoxicity in rats |
title_short | Preventive effect of fermented black ginseng against cisplatin-induced nephrotoxicity in rats |
title_sort | preventive effect of fermented black ginseng against cisplatin-induced nephrotoxicity in rats |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5386130/ https://www.ncbi.nlm.nih.gov/pubmed/28413323 http://dx.doi.org/10.1016/j.jgr.2016.03.001 |
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