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The transgenic cloned pig population with integrated and controllable GH expression that has higher feed efficiency and meat production

Sustained expression of the GH gene has been shown to have detrimental effects on the health of animals. In the current study, transgenic founder pigs, with controllable pig growth hormone (pGH) expression, were cloned via the handmade cloning method (HMC), and pGH expression levels were examined at...

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Autores principales: Ju, Huiming, Zhang, Jiaqing, Bai, Lijing, Mu, Yulian, Du, Yutao, Yang, Wenxian, Li, Yong, Sheng, Anzhi, Li, Kui
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group 2015
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5386205/
https://www.ncbi.nlm.nih.gov/pubmed/25959098
http://dx.doi.org/10.1038/srep10152
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author Ju, Huiming
Zhang, Jiaqing
Bai, Lijing
Mu, Yulian
Du, Yutao
Yang, Wenxian
Li, Yong
Sheng, Anzhi
Li, Kui
author_facet Ju, Huiming
Zhang, Jiaqing
Bai, Lijing
Mu, Yulian
Du, Yutao
Yang, Wenxian
Li, Yong
Sheng, Anzhi
Li, Kui
author_sort Ju, Huiming
collection PubMed
description Sustained expression of the GH gene has been shown to have detrimental effects on the health of animals. In the current study, transgenic founder pigs, with controllable pig growth hormone (pGH) expression, were cloned via the handmade cloning method (HMC), and pGH expression levels were examined at the cellular and organismal levels. The serum pGH levels in 3 founder male pigs were found to be significantly higher after induction with intramuscular injection of doxycycline (DOX) compared to baseline. A daily dose of DOX was administered via feed to these animals for a period of 65 to 155 days. The growth rate, feed efficiency and pGH serum concentration increased in the DOX-induced transgenic group compared with the other groups. 8 numbers of animals were euthanized and the dressing percentage, loin muscle and lean meat percentage were significantly higher in the DOX-induced F1 transgenic group compared with the other groups. In this study a large population of transgenic pigs, with integrated controllable expression of a transgene, was obtained. The transgenic pigs were healthy and normal in terms of reproductive capability. At the same time, feed efficiency was improved, production processes were accelerated and meat yield was increased.
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spelling pubmed-53862052017-04-14 The transgenic cloned pig population with integrated and controllable GH expression that has higher feed efficiency and meat production Ju, Huiming Zhang, Jiaqing Bai, Lijing Mu, Yulian Du, Yutao Yang, Wenxian Li, Yong Sheng, Anzhi Li, Kui Sci Rep Article Sustained expression of the GH gene has been shown to have detrimental effects on the health of animals. In the current study, transgenic founder pigs, with controllable pig growth hormone (pGH) expression, were cloned via the handmade cloning method (HMC), and pGH expression levels were examined at the cellular and organismal levels. The serum pGH levels in 3 founder male pigs were found to be significantly higher after induction with intramuscular injection of doxycycline (DOX) compared to baseline. A daily dose of DOX was administered via feed to these animals for a period of 65 to 155 days. The growth rate, feed efficiency and pGH serum concentration increased in the DOX-induced transgenic group compared with the other groups. 8 numbers of animals were euthanized and the dressing percentage, loin muscle and lean meat percentage were significantly higher in the DOX-induced F1 transgenic group compared with the other groups. In this study a large population of transgenic pigs, with integrated controllable expression of a transgene, was obtained. The transgenic pigs were healthy and normal in terms of reproductive capability. At the same time, feed efficiency was improved, production processes were accelerated and meat yield was increased. Nature Publishing Group 2015-05-11 /pmc/articles/PMC5386205/ /pubmed/25959098 http://dx.doi.org/10.1038/srep10152 Text en Copyright © 2015, Macmillan Publishers Limited http://creativecommons.org/licenses/by/4.0/ This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/
spellingShingle Article
Ju, Huiming
Zhang, Jiaqing
Bai, Lijing
Mu, Yulian
Du, Yutao
Yang, Wenxian
Li, Yong
Sheng, Anzhi
Li, Kui
The transgenic cloned pig population with integrated and controllable GH expression that has higher feed efficiency and meat production
title The transgenic cloned pig population with integrated and controllable GH expression that has higher feed efficiency and meat production
title_full The transgenic cloned pig population with integrated and controllable GH expression that has higher feed efficiency and meat production
title_fullStr The transgenic cloned pig population with integrated and controllable GH expression that has higher feed efficiency and meat production
title_full_unstemmed The transgenic cloned pig population with integrated and controllable GH expression that has higher feed efficiency and meat production
title_short The transgenic cloned pig population with integrated and controllable GH expression that has higher feed efficiency and meat production
title_sort transgenic cloned pig population with integrated and controllable gh expression that has higher feed efficiency and meat production
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5386205/
https://www.ncbi.nlm.nih.gov/pubmed/25959098
http://dx.doi.org/10.1038/srep10152
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