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The transgenic cloned pig population with integrated and controllable GH expression that has higher feed efficiency and meat production
Sustained expression of the GH gene has been shown to have detrimental effects on the health of animals. In the current study, transgenic founder pigs, with controllable pig growth hormone (pGH) expression, were cloned via the handmade cloning method (HMC), and pGH expression levels were examined at...
Autores principales: | , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Nature Publishing Group
2015
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5386205/ https://www.ncbi.nlm.nih.gov/pubmed/25959098 http://dx.doi.org/10.1038/srep10152 |
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author | Ju, Huiming Zhang, Jiaqing Bai, Lijing Mu, Yulian Du, Yutao Yang, Wenxian Li, Yong Sheng, Anzhi Li, Kui |
author_facet | Ju, Huiming Zhang, Jiaqing Bai, Lijing Mu, Yulian Du, Yutao Yang, Wenxian Li, Yong Sheng, Anzhi Li, Kui |
author_sort | Ju, Huiming |
collection | PubMed |
description | Sustained expression of the GH gene has been shown to have detrimental effects on the health of animals. In the current study, transgenic founder pigs, with controllable pig growth hormone (pGH) expression, were cloned via the handmade cloning method (HMC), and pGH expression levels were examined at the cellular and organismal levels. The serum pGH levels in 3 founder male pigs were found to be significantly higher after induction with intramuscular injection of doxycycline (DOX) compared to baseline. A daily dose of DOX was administered via feed to these animals for a period of 65 to 155 days. The growth rate, feed efficiency and pGH serum concentration increased in the DOX-induced transgenic group compared with the other groups. 8 numbers of animals were euthanized and the dressing percentage, loin muscle and lean meat percentage were significantly higher in the DOX-induced F1 transgenic group compared with the other groups. In this study a large population of transgenic pigs, with integrated controllable expression of a transgene, was obtained. The transgenic pigs were healthy and normal in terms of reproductive capability. At the same time, feed efficiency was improved, production processes were accelerated and meat yield was increased. |
format | Online Article Text |
id | pubmed-5386205 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2015 |
publisher | Nature Publishing Group |
record_format | MEDLINE/PubMed |
spelling | pubmed-53862052017-04-14 The transgenic cloned pig population with integrated and controllable GH expression that has higher feed efficiency and meat production Ju, Huiming Zhang, Jiaqing Bai, Lijing Mu, Yulian Du, Yutao Yang, Wenxian Li, Yong Sheng, Anzhi Li, Kui Sci Rep Article Sustained expression of the GH gene has been shown to have detrimental effects on the health of animals. In the current study, transgenic founder pigs, with controllable pig growth hormone (pGH) expression, were cloned via the handmade cloning method (HMC), and pGH expression levels were examined at the cellular and organismal levels. The serum pGH levels in 3 founder male pigs were found to be significantly higher after induction with intramuscular injection of doxycycline (DOX) compared to baseline. A daily dose of DOX was administered via feed to these animals for a period of 65 to 155 days. The growth rate, feed efficiency and pGH serum concentration increased in the DOX-induced transgenic group compared with the other groups. 8 numbers of animals were euthanized and the dressing percentage, loin muscle and lean meat percentage were significantly higher in the DOX-induced F1 transgenic group compared with the other groups. In this study a large population of transgenic pigs, with integrated controllable expression of a transgene, was obtained. The transgenic pigs were healthy and normal in terms of reproductive capability. At the same time, feed efficiency was improved, production processes were accelerated and meat yield was increased. Nature Publishing Group 2015-05-11 /pmc/articles/PMC5386205/ /pubmed/25959098 http://dx.doi.org/10.1038/srep10152 Text en Copyright © 2015, Macmillan Publishers Limited http://creativecommons.org/licenses/by/4.0/ This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/ |
spellingShingle | Article Ju, Huiming Zhang, Jiaqing Bai, Lijing Mu, Yulian Du, Yutao Yang, Wenxian Li, Yong Sheng, Anzhi Li, Kui The transgenic cloned pig population with integrated and controllable GH expression that has higher feed efficiency and meat production |
title | The transgenic cloned pig population with integrated and controllable GH expression that has higher feed efficiency and meat production |
title_full | The transgenic cloned pig population with integrated and controllable GH expression that has higher feed efficiency and meat production |
title_fullStr | The transgenic cloned pig population with integrated and controllable GH expression that has higher feed efficiency and meat production |
title_full_unstemmed | The transgenic cloned pig population with integrated and controllable GH expression that has higher feed efficiency and meat production |
title_short | The transgenic cloned pig population with integrated and controllable GH expression that has higher feed efficiency and meat production |
title_sort | transgenic cloned pig population with integrated and controllable gh expression that has higher feed efficiency and meat production |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5386205/ https://www.ncbi.nlm.nih.gov/pubmed/25959098 http://dx.doi.org/10.1038/srep10152 |
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