Cargando…
Angiogenesis-related genes may be a more important factor than matrix metalloproteinases in bronchopulmonary dysplasia development
We characterized the expression profile of angiogenesis-related genes (ARG) and matrix metalloproteinase (MMP) genes in preterm infants, with and without bronchopulmonary dysplasia (BPD). We reanalyzed a gene expression dataset for preterm infants from the Gene Expression Omnibus database using the...
Autores principales: | , , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Impact Journals LLC
2017
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5386638/ https://www.ncbi.nlm.nih.gov/pubmed/28103583 http://dx.doi.org/10.18632/oncotarget.14722 |
_version_ | 1782520806840991744 |
---|---|
author | Yang, Min Chen, Bo-Lin Huang, Jian-Bao Meng, Yan-Ni Duan, Xiao-Jun Chen, Lu Li, Lin-Rui Chen, Yan-Ping |
author_facet | Yang, Min Chen, Bo-Lin Huang, Jian-Bao Meng, Yan-Ni Duan, Xiao-Jun Chen, Lu Li, Lin-Rui Chen, Yan-Ping |
author_sort | Yang, Min |
collection | PubMed |
description | We characterized the expression profile of angiogenesis-related genes (ARG) and matrix metalloproteinase (MMP) genes in preterm infants, with and without bronchopulmonary dysplasia (BPD). We reanalyzed a gene expression dataset for preterm infants from the Gene Expression Omnibus database using the Gene-Cloud of Biotechnology Information platform. A total of 1,652 genes were differentially (1.2-fold change) expressed: 811 were highly expressed in infants with BPD, and 841 were highly expressed in those without BPD. Twenty-eight and 11 ARGs were upregulated in infants with and without BPD, respectively. Among 27 detected MMPs and TIMPs, MMP8, MMP9, MMP25, TIMP2 and TIMP3 were differently expressed. Levels of THBS1, MMP8, MMP9, MMP25, TIMP2 and TIMP3 increased as severity of BPD and retinopathy of prematurity (ROP) increased, whereas ETS1, LEF1 and SPOCK2 exhibited the opposite trend. Expression of ETS1 and LEF1 had a fitting rate of R(2) = 0.849 and P < 0.001. ELISAs showed a positive correlation between THBS1 and CD36 (receptor of THBS1) levels in serum samples from preterm infants. Our study indicates that the upregulation of THBS1 and downregulation of ETS1, LEF1 promotes BPD in preterm infants by disrupting blood vessel formation rather than by dysregulation of MMPs and TIMPs. |
format | Online Article Text |
id | pubmed-5386638 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2017 |
publisher | Impact Journals LLC |
record_format | MEDLINE/PubMed |
spelling | pubmed-53866382017-04-26 Angiogenesis-related genes may be a more important factor than matrix metalloproteinases in bronchopulmonary dysplasia development Yang, Min Chen, Bo-Lin Huang, Jian-Bao Meng, Yan-Ni Duan, Xiao-Jun Chen, Lu Li, Lin-Rui Chen, Yan-Ping Oncotarget Research Paper: Pathology We characterized the expression profile of angiogenesis-related genes (ARG) and matrix metalloproteinase (MMP) genes in preterm infants, with and without bronchopulmonary dysplasia (BPD). We reanalyzed a gene expression dataset for preterm infants from the Gene Expression Omnibus database using the Gene-Cloud of Biotechnology Information platform. A total of 1,652 genes were differentially (1.2-fold change) expressed: 811 were highly expressed in infants with BPD, and 841 were highly expressed in those without BPD. Twenty-eight and 11 ARGs were upregulated in infants with and without BPD, respectively. Among 27 detected MMPs and TIMPs, MMP8, MMP9, MMP25, TIMP2 and TIMP3 were differently expressed. Levels of THBS1, MMP8, MMP9, MMP25, TIMP2 and TIMP3 increased as severity of BPD and retinopathy of prematurity (ROP) increased, whereas ETS1, LEF1 and SPOCK2 exhibited the opposite trend. Expression of ETS1 and LEF1 had a fitting rate of R(2) = 0.849 and P < 0.001. ELISAs showed a positive correlation between THBS1 and CD36 (receptor of THBS1) levels in serum samples from preterm infants. Our study indicates that the upregulation of THBS1 and downregulation of ETS1, LEF1 promotes BPD in preterm infants by disrupting blood vessel formation rather than by dysregulation of MMPs and TIMPs. Impact Journals LLC 2017-01-18 /pmc/articles/PMC5386638/ /pubmed/28103583 http://dx.doi.org/10.18632/oncotarget.14722 Text en Copyright: © 2017 Yang et al. http://creativecommons.org/licenses/by/3.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. |
spellingShingle | Research Paper: Pathology Yang, Min Chen, Bo-Lin Huang, Jian-Bao Meng, Yan-Ni Duan, Xiao-Jun Chen, Lu Li, Lin-Rui Chen, Yan-Ping Angiogenesis-related genes may be a more important factor than matrix metalloproteinases in bronchopulmonary dysplasia development |
title | Angiogenesis-related genes may be a more important factor than matrix metalloproteinases in bronchopulmonary dysplasia development |
title_full | Angiogenesis-related genes may be a more important factor than matrix metalloproteinases in bronchopulmonary dysplasia development |
title_fullStr | Angiogenesis-related genes may be a more important factor than matrix metalloproteinases in bronchopulmonary dysplasia development |
title_full_unstemmed | Angiogenesis-related genes may be a more important factor than matrix metalloproteinases in bronchopulmonary dysplasia development |
title_short | Angiogenesis-related genes may be a more important factor than matrix metalloproteinases in bronchopulmonary dysplasia development |
title_sort | angiogenesis-related genes may be a more important factor than matrix metalloproteinases in bronchopulmonary dysplasia development |
topic | Research Paper: Pathology |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5386638/ https://www.ncbi.nlm.nih.gov/pubmed/28103583 http://dx.doi.org/10.18632/oncotarget.14722 |
work_keys_str_mv | AT yangmin angiogenesisrelatedgenesmaybeamoreimportantfactorthanmatrixmetalloproteinasesinbronchopulmonarydysplasiadevelopment AT chenbolin angiogenesisrelatedgenesmaybeamoreimportantfactorthanmatrixmetalloproteinasesinbronchopulmonarydysplasiadevelopment AT huangjianbao angiogenesisrelatedgenesmaybeamoreimportantfactorthanmatrixmetalloproteinasesinbronchopulmonarydysplasiadevelopment AT mengyanni angiogenesisrelatedgenesmaybeamoreimportantfactorthanmatrixmetalloproteinasesinbronchopulmonarydysplasiadevelopment AT duanxiaojun angiogenesisrelatedgenesmaybeamoreimportantfactorthanmatrixmetalloproteinasesinbronchopulmonarydysplasiadevelopment AT chenlu angiogenesisrelatedgenesmaybeamoreimportantfactorthanmatrixmetalloproteinasesinbronchopulmonarydysplasiadevelopment AT lilinrui angiogenesisrelatedgenesmaybeamoreimportantfactorthanmatrixmetalloproteinasesinbronchopulmonarydysplasiadevelopment AT chenyanping angiogenesisrelatedgenesmaybeamoreimportantfactorthanmatrixmetalloproteinasesinbronchopulmonarydysplasiadevelopment |