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Angiogenesis-related genes may be a more important factor than matrix metalloproteinases in bronchopulmonary dysplasia development

We characterized the expression profile of angiogenesis-related genes (ARG) and matrix metalloproteinase (MMP) genes in preterm infants, with and without bronchopulmonary dysplasia (BPD). We reanalyzed a gene expression dataset for preterm infants from the Gene Expression Omnibus database using the...

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Autores principales: Yang, Min, Chen, Bo-Lin, Huang, Jian-Bao, Meng, Yan-Ni, Duan, Xiao-Jun, Chen, Lu, Li, Lin-Rui, Chen, Yan-Ping
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Impact Journals LLC 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5386638/
https://www.ncbi.nlm.nih.gov/pubmed/28103583
http://dx.doi.org/10.18632/oncotarget.14722
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author Yang, Min
Chen, Bo-Lin
Huang, Jian-Bao
Meng, Yan-Ni
Duan, Xiao-Jun
Chen, Lu
Li, Lin-Rui
Chen, Yan-Ping
author_facet Yang, Min
Chen, Bo-Lin
Huang, Jian-Bao
Meng, Yan-Ni
Duan, Xiao-Jun
Chen, Lu
Li, Lin-Rui
Chen, Yan-Ping
author_sort Yang, Min
collection PubMed
description We characterized the expression profile of angiogenesis-related genes (ARG) and matrix metalloproteinase (MMP) genes in preterm infants, with and without bronchopulmonary dysplasia (BPD). We reanalyzed a gene expression dataset for preterm infants from the Gene Expression Omnibus database using the Gene-Cloud of Biotechnology Information platform. A total of 1,652 genes were differentially (1.2-fold change) expressed: 811 were highly expressed in infants with BPD, and 841 were highly expressed in those without BPD. Twenty-eight and 11 ARGs were upregulated in infants with and without BPD, respectively. Among 27 detected MMPs and TIMPs, MMP8, MMP9, MMP25, TIMP2 and TIMP3 were differently expressed. Levels of THBS1, MMP8, MMP9, MMP25, TIMP2 and TIMP3 increased as severity of BPD and retinopathy of prematurity (ROP) increased, whereas ETS1, LEF1 and SPOCK2 exhibited the opposite trend. Expression of ETS1 and LEF1 had a fitting rate of R(2) = 0.849 and P < 0.001. ELISAs showed a positive correlation between THBS1 and CD36 (receptor of THBS1) levels in serum samples from preterm infants. Our study indicates that the upregulation of THBS1 and downregulation of ETS1, LEF1 promotes BPD in preterm infants by disrupting blood vessel formation rather than by dysregulation of MMPs and TIMPs.
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spelling pubmed-53866382017-04-26 Angiogenesis-related genes may be a more important factor than matrix metalloproteinases in bronchopulmonary dysplasia development Yang, Min Chen, Bo-Lin Huang, Jian-Bao Meng, Yan-Ni Duan, Xiao-Jun Chen, Lu Li, Lin-Rui Chen, Yan-Ping Oncotarget Research Paper: Pathology We characterized the expression profile of angiogenesis-related genes (ARG) and matrix metalloproteinase (MMP) genes in preterm infants, with and without bronchopulmonary dysplasia (BPD). We reanalyzed a gene expression dataset for preterm infants from the Gene Expression Omnibus database using the Gene-Cloud of Biotechnology Information platform. A total of 1,652 genes were differentially (1.2-fold change) expressed: 811 were highly expressed in infants with BPD, and 841 were highly expressed in those without BPD. Twenty-eight and 11 ARGs were upregulated in infants with and without BPD, respectively. Among 27 detected MMPs and TIMPs, MMP8, MMP9, MMP25, TIMP2 and TIMP3 were differently expressed. Levels of THBS1, MMP8, MMP9, MMP25, TIMP2 and TIMP3 increased as severity of BPD and retinopathy of prematurity (ROP) increased, whereas ETS1, LEF1 and SPOCK2 exhibited the opposite trend. Expression of ETS1 and LEF1 had a fitting rate of R(2) = 0.849 and P < 0.001. ELISAs showed a positive correlation between THBS1 and CD36 (receptor of THBS1) levels in serum samples from preterm infants. Our study indicates that the upregulation of THBS1 and downregulation of ETS1, LEF1 promotes BPD in preterm infants by disrupting blood vessel formation rather than by dysregulation of MMPs and TIMPs. Impact Journals LLC 2017-01-18 /pmc/articles/PMC5386638/ /pubmed/28103583 http://dx.doi.org/10.18632/oncotarget.14722 Text en Copyright: © 2017 Yang et al. http://creativecommons.org/licenses/by/3.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
spellingShingle Research Paper: Pathology
Yang, Min
Chen, Bo-Lin
Huang, Jian-Bao
Meng, Yan-Ni
Duan, Xiao-Jun
Chen, Lu
Li, Lin-Rui
Chen, Yan-Ping
Angiogenesis-related genes may be a more important factor than matrix metalloproteinases in bronchopulmonary dysplasia development
title Angiogenesis-related genes may be a more important factor than matrix metalloproteinases in bronchopulmonary dysplasia development
title_full Angiogenesis-related genes may be a more important factor than matrix metalloproteinases in bronchopulmonary dysplasia development
title_fullStr Angiogenesis-related genes may be a more important factor than matrix metalloproteinases in bronchopulmonary dysplasia development
title_full_unstemmed Angiogenesis-related genes may be a more important factor than matrix metalloproteinases in bronchopulmonary dysplasia development
title_short Angiogenesis-related genes may be a more important factor than matrix metalloproteinases in bronchopulmonary dysplasia development
title_sort angiogenesis-related genes may be a more important factor than matrix metalloproteinases in bronchopulmonary dysplasia development
topic Research Paper: Pathology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5386638/
https://www.ncbi.nlm.nih.gov/pubmed/28103583
http://dx.doi.org/10.18632/oncotarget.14722
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