Expression and mutational analysis of c-CBL and its relationship to the MET receptor in head and neck squamous cell carcinoma

MET is frequently overexpressed in head and neck squamous cell carcinoma (HNSCC) and degraded by c-CBL E3-ubiquitin ligase. We investigated genetic variations of c-CBL in HNSCC and the relationship between c-CBL and MET expression. High MET, low c-CBL expression was detected in 10 cell lines and 73...

Descripción completa

Detalles Bibliográficos
Autores principales: Rolle, Cleo E., Tan, Yi-Hung Carol, Seiwert, Tanguy Y., Vora, Sapana, Kanteti, Rajani, Hasina, Rifat, Carey, George B., Surati, Mosmi, Weichselbaum, Ralph R., Lingen, Mark W., Vokes, Everett E., Salgia, Ravi
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Impact Journals LLC 2016
Materias:
Research Paper
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5386642/
https://www.ncbi.nlm.nih.gov/pubmed/27244893
http://dx.doi.org/10.18632/oncotarget.9640
_version_ 1782520807735427072
author Rolle, Cleo E.
Tan, Yi-Hung Carol
Seiwert, Tanguy Y.
Vora, Sapana
Kanteti, Rajani
Hasina, Rifat
Carey, George B.
Surati, Mosmi
Weichselbaum, Ralph R.
Lingen, Mark W.
Vokes, Everett E.
Salgia, Ravi
author_facet Rolle, Cleo E.
Tan, Yi-Hung Carol
Seiwert, Tanguy Y.
Vora, Sapana
Kanteti, Rajani
Hasina, Rifat
Carey, George B.
Surati, Mosmi
Weichselbaum, Ralph R.
Lingen, Mark W.
Vokes, Everett E.
Salgia, Ravi
author_sort Rolle, Cleo E.
collection PubMed
description MET is frequently overexpressed in head and neck squamous cell carcinoma (HNSCC) and degraded by c-CBL E3-ubiquitin ligase. We investigated genetic variations of c-CBL in HNSCC and the relationship between c-CBL and MET expression. High MET, low c-CBL expression was detected in 10 cell lines and 73 tumor tissues. Two novel mutations (L254S, L281F), and the single nucleotide polymorphism (SNP) P782L were identified from archival tumor tissues. 27.3% of loss of heterozygosity was found at CBL locus. Ectopic expression of wild-type c-CBL in SCC-35 cells downregulated MET expression and decreased cell viability. These results suggest MET overexpression is related to altered c-CBL expression, which may influence tumorigenesis.
format Online
Article
Text
id pubmed-5386642
institution National Center for Biotechnology Information
language English
publishDate 2016
publisher Impact Journals LLC
record_format MEDLINE/PubMed
spelling pubmed-53866422017-04-26 Expression and mutational analysis of c-CBL and its relationship to the MET receptor in head and neck squamous cell carcinoma Rolle, Cleo E. Tan, Yi-Hung Carol Seiwert, Tanguy Y. Vora, Sapana Kanteti, Rajani Hasina, Rifat Carey, George B. Surati, Mosmi Weichselbaum, Ralph R. Lingen, Mark W. Vokes, Everett E. Salgia, Ravi Oncotarget Research Paper MET is frequently overexpressed in head and neck squamous cell carcinoma (HNSCC) and degraded by c-CBL E3-ubiquitin ligase. We investigated genetic variations of c-CBL in HNSCC and the relationship between c-CBL and MET expression. High MET, low c-CBL expression was detected in 10 cell lines and 73 tumor tissues. Two novel mutations (L254S, L281F), and the single nucleotide polymorphism (SNP) P782L were identified from archival tumor tissues. 27.3% of loss of heterozygosity was found at CBL locus. Ectopic expression of wild-type c-CBL in SCC-35 cells downregulated MET expression and decreased cell viability. These results suggest MET overexpression is related to altered c-CBL expression, which may influence tumorigenesis. Impact Journals LLC 2016-05-26 /pmc/articles/PMC5386642/ /pubmed/27244893 http://dx.doi.org/10.18632/oncotarget.9640 Text en Copyright: © 2017 Rolle et al. http://creativecommons.org/licenses/by/3.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
spellingShingle Research Paper
Rolle, Cleo E.
Tan, Yi-Hung Carol
Seiwert, Tanguy Y.
Vora, Sapana
Kanteti, Rajani
Hasina, Rifat
Carey, George B.
Surati, Mosmi
Weichselbaum, Ralph R.
Lingen, Mark W.
Vokes, Everett E.
Salgia, Ravi
Expression and mutational analysis of c-CBL and its relationship to the MET receptor in head and neck squamous cell carcinoma
title Expression and mutational analysis of c-CBL and its relationship to the MET receptor in head and neck squamous cell carcinoma
title_full Expression and mutational analysis of c-CBL and its relationship to the MET receptor in head and neck squamous cell carcinoma
title_fullStr Expression and mutational analysis of c-CBL and its relationship to the MET receptor in head and neck squamous cell carcinoma
title_full_unstemmed Expression and mutational analysis of c-CBL and its relationship to the MET receptor in head and neck squamous cell carcinoma
title_short Expression and mutational analysis of c-CBL and its relationship to the MET receptor in head and neck squamous cell carcinoma
title_sort expression and mutational analysis of c-cbl and its relationship to the met receptor in head and neck squamous cell carcinoma
topic Research Paper
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5386642/
https://www.ncbi.nlm.nih.gov/pubmed/27244893
http://dx.doi.org/10.18632/oncotarget.9640
work_keys_str_mv AT rollecleoe expressionandmutationalanalysisofccblanditsrelationshiptothemetreceptorinheadandnecksquamouscellcarcinoma
AT tanyihungcarol expressionandmutationalanalysisofccblanditsrelationshiptothemetreceptorinheadandnecksquamouscellcarcinoma
AT seiwerttanguyy expressionandmutationalanalysisofccblanditsrelationshiptothemetreceptorinheadandnecksquamouscellcarcinoma
AT vorasapana expressionandmutationalanalysisofccblanditsrelationshiptothemetreceptorinheadandnecksquamouscellcarcinoma
AT kantetirajani expressionandmutationalanalysisofccblanditsrelationshiptothemetreceptorinheadandnecksquamouscellcarcinoma
AT hasinarifat expressionandmutationalanalysisofccblanditsrelationshiptothemetreceptorinheadandnecksquamouscellcarcinoma
AT careygeorgeb expressionandmutationalanalysisofccblanditsrelationshiptothemetreceptorinheadandnecksquamouscellcarcinoma
AT suratimosmi expressionandmutationalanalysisofccblanditsrelationshiptothemetreceptorinheadandnecksquamouscellcarcinoma
AT weichselbaumralphr expressionandmutationalanalysisofccblanditsrelationshiptothemetreceptorinheadandnecksquamouscellcarcinoma
AT lingenmarkw expressionandmutationalanalysisofccblanditsrelationshiptothemetreceptorinheadandnecksquamouscellcarcinoma
AT vokeseverette expressionandmutationalanalysisofccblanditsrelationshiptothemetreceptorinheadandnecksquamouscellcarcinoma
AT salgiaravi expressionandmutationalanalysisofccblanditsrelationshiptothemetreceptorinheadandnecksquamouscellcarcinoma

Ejemplares similares