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The clinical significance and underlying correlation of pStat-3 and integrin αvβ6 expression in gallbladder cancer
BACKGROUND: Both phosphorylated signal transducer and activator of transcription 3(pStat-3) and integrin αvβ6 can play vital role in the development and progression of cancer. However, little is known about their expression correlation and clinical significance in gallbladder cancer(GBC). OBJECTIVE:...
Autores principales: | , , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Impact Journals LLC
2017
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5386698/ https://www.ncbi.nlm.nih.gov/pubmed/28061445 http://dx.doi.org/10.18632/oncotarget.14444 |
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author | Enyu, Liu Na, Wang Chuanzong, Zhao Ben, Wang Xiaojuan, Wu Yan, Wang Zequn, Li Jianguo, Hong Jiayong, Wang Benjia, Liang Cheng, Peng Min, Zhu Zongli, Zhang |
author_facet | Enyu, Liu Na, Wang Chuanzong, Zhao Ben, Wang Xiaojuan, Wu Yan, Wang Zequn, Li Jianguo, Hong Jiayong, Wang Benjia, Liang Cheng, Peng Min, Zhu Zongli, Zhang |
author_sort | Enyu, Liu |
collection | PubMed |
description | BACKGROUND: Both phosphorylated signal transducer and activator of transcription 3(pStat-3) and integrin αvβ6 can play vital role in the development and progression of cancer. However, little is known about their expression correlation and clinical significance in gallbladder cancer(GBC). OBJECTIVE: The aim of our present study was to investigate the expression of pStat-3 and integrin αvβ6, two proteins’ correlation and their clinical significance in GBC tissues. RESULTS: The expression of pStat-3 and integrin αvβ6 were both significantly associated with T stage, lymph node metastasis status, TNM stage (P=0.008, P=0.000, P=0.000 and P=0.036, P=0.001,P=0.000,respectively). IHC and Western blot showed their expressions in GBC tissues were higher than that in paraneoplastic tissues. Moderate positive correlation existed between the two proteins (r =0.349, P <0.001). The survival analysis by Kaplan-Meier and Cox regression model showed that GBC patients with pStat-3 or integrin αvβ6 positive expression had a significantly poorer 2-year survival rate (P = 0.002 and 0.000, the log-rank test, respectively), and either marker could act as unfavorable independent prognostic factors(RR=1.907, P=0.021 and RR=2.046, P=0.038). MATERIALS AND METHODS: The expression levels of pStat-3 and integrin αvβ6 were analyzed in GBC cancerous and paraneoplastic tissues of 97 cases via immunohistochemistry(IHC) and further validated by western blot method. Besides, SPSS software was used to observe their clinical significance as well as the two proteins’ correlation. CONCLUSION: pStat-3 and integrin αvβ6 were indicators of tumor's progression and poor prognosis of patients with GBC. And the further study involving them may provide a helpful therapeutic target in prevention and treatment of GBC patients. |
format | Online Article Text |
id | pubmed-5386698 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2017 |
publisher | Impact Journals LLC |
record_format | MEDLINE/PubMed |
spelling | pubmed-53866982017-04-26 The clinical significance and underlying correlation of pStat-3 and integrin αvβ6 expression in gallbladder cancer Enyu, Liu Na, Wang Chuanzong, Zhao Ben, Wang Xiaojuan, Wu Yan, Wang Zequn, Li Jianguo, Hong Jiayong, Wang Benjia, Liang Cheng, Peng Min, Zhu Zongli, Zhang Oncotarget Research Paper BACKGROUND: Both phosphorylated signal transducer and activator of transcription 3(pStat-3) and integrin αvβ6 can play vital role in the development and progression of cancer. However, little is known about their expression correlation and clinical significance in gallbladder cancer(GBC). OBJECTIVE: The aim of our present study was to investigate the expression of pStat-3 and integrin αvβ6, two proteins’ correlation and their clinical significance in GBC tissues. RESULTS: The expression of pStat-3 and integrin αvβ6 were both significantly associated with T stage, lymph node metastasis status, TNM stage (P=0.008, P=0.000, P=0.000 and P=0.036, P=0.001,P=0.000,respectively). IHC and Western blot showed their expressions in GBC tissues were higher than that in paraneoplastic tissues. Moderate positive correlation existed between the two proteins (r =0.349, P <0.001). The survival analysis by Kaplan-Meier and Cox regression model showed that GBC patients with pStat-3 or integrin αvβ6 positive expression had a significantly poorer 2-year survival rate (P = 0.002 and 0.000, the log-rank test, respectively), and either marker could act as unfavorable independent prognostic factors(RR=1.907, P=0.021 and RR=2.046, P=0.038). MATERIALS AND METHODS: The expression levels of pStat-3 and integrin αvβ6 were analyzed in GBC cancerous and paraneoplastic tissues of 97 cases via immunohistochemistry(IHC) and further validated by western blot method. Besides, SPSS software was used to observe their clinical significance as well as the two proteins’ correlation. CONCLUSION: pStat-3 and integrin αvβ6 were indicators of tumor's progression and poor prognosis of patients with GBC. And the further study involving them may provide a helpful therapeutic target in prevention and treatment of GBC patients. Impact Journals LLC 2017-01-02 /pmc/articles/PMC5386698/ /pubmed/28061445 http://dx.doi.org/10.18632/oncotarget.14444 Text en Copyright: © 2017 Enyu et al. http://creativecommons.org/licenses/by/3.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. |
spellingShingle | Research Paper Enyu, Liu Na, Wang Chuanzong, Zhao Ben, Wang Xiaojuan, Wu Yan, Wang Zequn, Li Jianguo, Hong Jiayong, Wang Benjia, Liang Cheng, Peng Min, Zhu Zongli, Zhang The clinical significance and underlying correlation of pStat-3 and integrin αvβ6 expression in gallbladder cancer |
title | The clinical significance and underlying correlation of pStat-3 and integrin αvβ6 expression in gallbladder cancer |
title_full | The clinical significance and underlying correlation of pStat-3 and integrin αvβ6 expression in gallbladder cancer |
title_fullStr | The clinical significance and underlying correlation of pStat-3 and integrin αvβ6 expression in gallbladder cancer |
title_full_unstemmed | The clinical significance and underlying correlation of pStat-3 and integrin αvβ6 expression in gallbladder cancer |
title_short | The clinical significance and underlying correlation of pStat-3 and integrin αvβ6 expression in gallbladder cancer |
title_sort | clinical significance and underlying correlation of pstat-3 and integrin αvβ6 expression in gallbladder cancer |
topic | Research Paper |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5386698/ https://www.ncbi.nlm.nih.gov/pubmed/28061445 http://dx.doi.org/10.18632/oncotarget.14444 |
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