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A Role of Central NELL2 in the Regulation of Feeding Behavior in Rats

A brain-enriched secreting signal peptide, NELL2, has been suggested to play multiple roles in the development, survival, and activity of neurons in mammal. We investigated here a possible involvement of central NELL2 in regulating feeding behavior and metabolism. In situ hybridization and an im-mun...

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Autores principales: Jeong, Jin Kwon, Kim, Jae Geun, Kim, Han Rae, Lee, Tae Hwan, Park, Jeong Woo, Lee, Byung Ju
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Korean Society for Molecular and Cellular Biology 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5386956/
https://www.ncbi.nlm.nih.gov/pubmed/28301916
http://dx.doi.org/10.14348/molcells.2017.2278
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author Jeong, Jin Kwon
Kim, Jae Geun
Kim, Han Rae
Lee, Tae Hwan
Park, Jeong Woo
Lee, Byung Ju
author_facet Jeong, Jin Kwon
Kim, Jae Geun
Kim, Han Rae
Lee, Tae Hwan
Park, Jeong Woo
Lee, Byung Ju
author_sort Jeong, Jin Kwon
collection PubMed
description A brain-enriched secreting signal peptide, NELL2, has been suggested to play multiple roles in the development, survival, and activity of neurons in mammal. We investigated here a possible involvement of central NELL2 in regulating feeding behavior and metabolism. In situ hybridization and an im-munohistochemical approach were used to determine expression of NELL2 as well as its colocalization with proopiomelanocortin (POMC) and neuropeptide Y (NPY) in the rat hypothalamus. To investigate the effect of NELL2 on feeding behavior, 2 nmole of antisense NELL2 oligodeoxynucleotide was administered into the lateral ventricle of adult male rat brains for 6 consecutive days, and changes in daily body weight, food, and water intake were monitored. Metabolic state-dependent NELL2 expression in the hypothalamus was tested in vivo using a fasting model. NELL2 was noticeably expressed in the hypothalamic nuclei controlling feeding behavior. Furthermore, all arcuatic POMC and NPY positive neurons produced NELL2. The NELL2 gene expression in the hypothalamus was up-regulated by fasting. However, NELL2 did not affect POMC and NPY gene expression in the hypothalamus. A blockade of NELL2 production in the hypothalamus led to a reduction in daily food intake, followed by a loss in body weight without a change in daily water intake in normal diet condition. NELL2 did not affect short-term hunger dependent appetite behavior. Our data suggests that hypothalamic NELL2 is associated with appetite behavior, and thus central NELL2 could be a new therapeutic target for obesity.
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spelling pubmed-53869562017-04-11 A Role of Central NELL2 in the Regulation of Feeding Behavior in Rats Jeong, Jin Kwon Kim, Jae Geun Kim, Han Rae Lee, Tae Hwan Park, Jeong Woo Lee, Byung Ju Mol Cells Article A brain-enriched secreting signal peptide, NELL2, has been suggested to play multiple roles in the development, survival, and activity of neurons in mammal. We investigated here a possible involvement of central NELL2 in regulating feeding behavior and metabolism. In situ hybridization and an im-munohistochemical approach were used to determine expression of NELL2 as well as its colocalization with proopiomelanocortin (POMC) and neuropeptide Y (NPY) in the rat hypothalamus. To investigate the effect of NELL2 on feeding behavior, 2 nmole of antisense NELL2 oligodeoxynucleotide was administered into the lateral ventricle of adult male rat brains for 6 consecutive days, and changes in daily body weight, food, and water intake were monitored. Metabolic state-dependent NELL2 expression in the hypothalamus was tested in vivo using a fasting model. NELL2 was noticeably expressed in the hypothalamic nuclei controlling feeding behavior. Furthermore, all arcuatic POMC and NPY positive neurons produced NELL2. The NELL2 gene expression in the hypothalamus was up-regulated by fasting. However, NELL2 did not affect POMC and NPY gene expression in the hypothalamus. A blockade of NELL2 production in the hypothalamus led to a reduction in daily food intake, followed by a loss in body weight without a change in daily water intake in normal diet condition. NELL2 did not affect short-term hunger dependent appetite behavior. Our data suggests that hypothalamic NELL2 is associated with appetite behavior, and thus central NELL2 could be a new therapeutic target for obesity. Korean Society for Molecular and Cellular Biology 2017-03-31 2017-03-15 /pmc/articles/PMC5386956/ /pubmed/28301916 http://dx.doi.org/10.14348/molcells.2017.2278 Text en © The Korean Society for Molecular and Cellular Biology. All rights reserved. This is an open-access article distributed under the terms of the Creative Commons Attribution-NonCommercial-ShareAlike 3.0 Unported License. To view a copy of this license, visit http://creativecommons.org/licenses/by-nc-sa/3.0/.
spellingShingle Article
Jeong, Jin Kwon
Kim, Jae Geun
Kim, Han Rae
Lee, Tae Hwan
Park, Jeong Woo
Lee, Byung Ju
A Role of Central NELL2 in the Regulation of Feeding Behavior in Rats
title A Role of Central NELL2 in the Regulation of Feeding Behavior in Rats
title_full A Role of Central NELL2 in the Regulation of Feeding Behavior in Rats
title_fullStr A Role of Central NELL2 in the Regulation of Feeding Behavior in Rats
title_full_unstemmed A Role of Central NELL2 in the Regulation of Feeding Behavior in Rats
title_short A Role of Central NELL2 in the Regulation of Feeding Behavior in Rats
title_sort role of central nell2 in the regulation of feeding behavior in rats
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5386956/
https://www.ncbi.nlm.nih.gov/pubmed/28301916
http://dx.doi.org/10.14348/molcells.2017.2278
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