MiR-186 Inhibited Migration of NSCLC via Targeting cdc42 and Effecting EMT Process

In this study, qRT-PCR was employed to identify that miR-186 expression level in NSCLC tissues are highly associated with lymph node metastasis. In addition, through the application of western blotting, luciferase assay and qRT-PCR, it was found that miR-186 targeted 3′UTR of cdc42 mRNA and down-reg...

Descripción completa

Detalles Bibliográficos
Autores principales: Dong, Ying, Jin, Xintian, Sun, Zhiqiang, Zhao, Yueming, Song, Xianjing
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Korean Society for Molecular and Cellular Biology 2017
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5386957/
https://www.ncbi.nlm.nih.gov/pubmed/28317368
http://dx.doi.org/10.14348/molcells.2017.2291
Descripción
Sumario:In this study, qRT-PCR was employed to identify that miR-186 expression level in NSCLC tissues are highly associated with lymph node metastasis. In addition, through the application of western blotting, luciferase assay and qRT-PCR, it was found that miR-186 targeted 3′UTR of cdc42 mRNA and down-regulated cdc42 protein level in a post-transcriptional manner. Transwell assay indicated that cdc42 partially reversed the effect of miR-186 mimics. Besides, miR-186 was proved to regulate EMT by influencing biomarkers of this process and cell adhesion ability. Thus, miR-186 is a potential target for NSCLC therapy. miR-186 is proposed to be one of tumor-suppressors and may serve as a therapeutic target in NSCLC treatment.

Ejemplares similares