MiR-186 Inhibited Migration of NSCLC via Targeting cdc42 and Effecting EMT Process

In this study, qRT-PCR was employed to identify that miR-186 expression level in NSCLC tissues are highly associated with lymph node metastasis. In addition, through the application of western blotting, luciferase assay and qRT-PCR, it was found that miR-186 targeted 3′UTR of cdc42 mRNA and down-reg...

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Autores principales: Dong, Ying, Jin, Xintian, Sun, Zhiqiang, Zhao, Yueming, Song, Xianjing
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Korean Society for Molecular and Cellular Biology 2017
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5386957/
https://www.ncbi.nlm.nih.gov/pubmed/28317368
http://dx.doi.org/10.14348/molcells.2017.2291
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author Dong, Ying
Jin, Xintian
Sun, Zhiqiang
Zhao, Yueming
Song, Xianjing
author_facet Dong, Ying
Jin, Xintian
Sun, Zhiqiang
Zhao, Yueming
Song, Xianjing
author_sort Dong, Ying
collection PubMed
description In this study, qRT-PCR was employed to identify that miR-186 expression level in NSCLC tissues are highly associated with lymph node metastasis. In addition, through the application of western blotting, luciferase assay and qRT-PCR, it was found that miR-186 targeted 3′UTR of cdc42 mRNA and down-regulated cdc42 protein level in a post-transcriptional manner. Transwell assay indicated that cdc42 partially reversed the effect of miR-186 mimics. Besides, miR-186 was proved to regulate EMT by influencing biomarkers of this process and cell adhesion ability. Thus, miR-186 is a potential target for NSCLC therapy. miR-186 is proposed to be one of tumor-suppressors and may serve as a therapeutic target in NSCLC treatment.
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spelling pubmed-53869572017-04-11 MiR-186 Inhibited Migration of NSCLC via Targeting cdc42 and Effecting EMT Process Dong, Ying Jin, Xintian Sun, Zhiqiang Zhao, Yueming Song, Xianjing Mol Cells Article In this study, qRT-PCR was employed to identify that miR-186 expression level in NSCLC tissues are highly associated with lymph node metastasis. In addition, through the application of western blotting, luciferase assay and qRT-PCR, it was found that miR-186 targeted 3′UTR of cdc42 mRNA and down-regulated cdc42 protein level in a post-transcriptional manner. Transwell assay indicated that cdc42 partially reversed the effect of miR-186 mimics. Besides, miR-186 was proved to regulate EMT by influencing biomarkers of this process and cell adhesion ability. Thus, miR-186 is a potential target for NSCLC therapy. miR-186 is proposed to be one of tumor-suppressors and may serve as a therapeutic target in NSCLC treatment. Korean Society for Molecular and Cellular Biology 2017-03-31 2017-03-20 /pmc/articles/PMC5386957/ /pubmed/28317368 http://dx.doi.org/10.14348/molcells.2017.2291 Text en © The Korean Society for Molecular and Cellular Biology. All rights reserved. This is an open-access article distributed under the terms of the Creative Commons Attribution-NonCommercial-ShareAlike 3.0 Unported License. To view a copy of this license, visit http://creativecommons.org/licenses/by-nc-sa/3.0/.
spellingShingle Article
Dong, Ying
Jin, Xintian
Sun, Zhiqiang
Zhao, Yueming
Song, Xianjing
MiR-186 Inhibited Migration of NSCLC via Targeting cdc42 and Effecting EMT Process
title MiR-186 Inhibited Migration of NSCLC via Targeting cdc42 and Effecting EMT Process
title_full MiR-186 Inhibited Migration of NSCLC via Targeting cdc42 and Effecting EMT Process
title_fullStr MiR-186 Inhibited Migration of NSCLC via Targeting cdc42 and Effecting EMT Process
title_full_unstemmed MiR-186 Inhibited Migration of NSCLC via Targeting cdc42 and Effecting EMT Process
title_short MiR-186 Inhibited Migration of NSCLC via Targeting cdc42 and Effecting EMT Process
title_sort mir-186 inhibited migration of nsclc via targeting cdc42 and effecting emt process
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5386957/
https://www.ncbi.nlm.nih.gov/pubmed/28317368
http://dx.doi.org/10.14348/molcells.2017.2291
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