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Deletion of Lipoteichoic Acid Synthase Impacts Expression of Genes Encoding Cell Surface Proteins in Lactobacillus acidophilus

Lactobacillus acidophilus NCFM is a well-characterized probiotic microorganism, supported by a decade of genomic and functional phenotypic investigations. L. acidophilus deficient in lipoteichoic acid (LTA), a major immunostimulant in Gram-positive bacteria, has been shown to shift immune system res...

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Autores principales: Selle, Kurt, Goh, Yong J., Johnson, Brant R., O’Flaherty, Sarah, Andersen, Joakim M., Barrangou, Rodolphe, Klaenhammer, Todd R.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5387067/
https://www.ncbi.nlm.nih.gov/pubmed/28443071
http://dx.doi.org/10.3389/fmicb.2017.00553
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author Selle, Kurt
Goh, Yong J.
Johnson, Brant R.
O’Flaherty, Sarah
Andersen, Joakim M.
Barrangou, Rodolphe
Klaenhammer, Todd R.
author_facet Selle, Kurt
Goh, Yong J.
Johnson, Brant R.
O’Flaherty, Sarah
Andersen, Joakim M.
Barrangou, Rodolphe
Klaenhammer, Todd R.
author_sort Selle, Kurt
collection PubMed
description Lactobacillus acidophilus NCFM is a well-characterized probiotic microorganism, supported by a decade of genomic and functional phenotypic investigations. L. acidophilus deficient in lipoteichoic acid (LTA), a major immunostimulant in Gram-positive bacteria, has been shown to shift immune system responses in animal disease models. However, the pleiotropic effects of removing LTA from the cell surface in lactobacilli are unknown. In this study, we surveyed the global transcriptional and extracellular protein profiles of two strains of L. acidophilus deficient in LTA. Twenty-four differentially expressed genes specific to the LTA-deficient strains were identified, including a predicted heavy metal resistance operon and several putative peptidoglycan hydrolases. Cell morphology and manganese sensitivity phenotypes were assessed in relation to the putative functions of differentially expressed genes. LTA-deficient L. acidophilus exhibited elongated cellular morphology and their growth was severely inhibited by elevated manganese concentrations. Exoproteomic surveys revealed distinct changes in the composition and relative abundances of several extracellular proteins and showed a bias of intracellular proteins in LTA-deficient strains of L. acidophilus. Taken together, these results elucidate the impact of ltaS deletion on the transcriptome and extracellular proteins of L. acidophilus, suggesting roles of LTA in cell morphology and ion homeostasis as a structural component of the Gram positive cell wall.
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spelling pubmed-53870672017-04-25 Deletion of Lipoteichoic Acid Synthase Impacts Expression of Genes Encoding Cell Surface Proteins in Lactobacillus acidophilus Selle, Kurt Goh, Yong J. Johnson, Brant R. O’Flaherty, Sarah Andersen, Joakim M. Barrangou, Rodolphe Klaenhammer, Todd R. Front Microbiol Microbiology Lactobacillus acidophilus NCFM is a well-characterized probiotic microorganism, supported by a decade of genomic and functional phenotypic investigations. L. acidophilus deficient in lipoteichoic acid (LTA), a major immunostimulant in Gram-positive bacteria, has been shown to shift immune system responses in animal disease models. However, the pleiotropic effects of removing LTA from the cell surface in lactobacilli are unknown. In this study, we surveyed the global transcriptional and extracellular protein profiles of two strains of L. acidophilus deficient in LTA. Twenty-four differentially expressed genes specific to the LTA-deficient strains were identified, including a predicted heavy metal resistance operon and several putative peptidoglycan hydrolases. Cell morphology and manganese sensitivity phenotypes were assessed in relation to the putative functions of differentially expressed genes. LTA-deficient L. acidophilus exhibited elongated cellular morphology and their growth was severely inhibited by elevated manganese concentrations. Exoproteomic surveys revealed distinct changes in the composition and relative abundances of several extracellular proteins and showed a bias of intracellular proteins in LTA-deficient strains of L. acidophilus. Taken together, these results elucidate the impact of ltaS deletion on the transcriptome and extracellular proteins of L. acidophilus, suggesting roles of LTA in cell morphology and ion homeostasis as a structural component of the Gram positive cell wall. Frontiers Media S.A. 2017-04-11 /pmc/articles/PMC5387067/ /pubmed/28443071 http://dx.doi.org/10.3389/fmicb.2017.00553 Text en Copyright © 2017 Selle, Goh, Johnson, O’Flaherty, Andersen, Barrangou and Klaenhammer. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Microbiology
Selle, Kurt
Goh, Yong J.
Johnson, Brant R.
O’Flaherty, Sarah
Andersen, Joakim M.
Barrangou, Rodolphe
Klaenhammer, Todd R.
Deletion of Lipoteichoic Acid Synthase Impacts Expression of Genes Encoding Cell Surface Proteins in Lactobacillus acidophilus
title Deletion of Lipoteichoic Acid Synthase Impacts Expression of Genes Encoding Cell Surface Proteins in Lactobacillus acidophilus
title_full Deletion of Lipoteichoic Acid Synthase Impacts Expression of Genes Encoding Cell Surface Proteins in Lactobacillus acidophilus
title_fullStr Deletion of Lipoteichoic Acid Synthase Impacts Expression of Genes Encoding Cell Surface Proteins in Lactobacillus acidophilus
title_full_unstemmed Deletion of Lipoteichoic Acid Synthase Impacts Expression of Genes Encoding Cell Surface Proteins in Lactobacillus acidophilus
title_short Deletion of Lipoteichoic Acid Synthase Impacts Expression of Genes Encoding Cell Surface Proteins in Lactobacillus acidophilus
title_sort deletion of lipoteichoic acid synthase impacts expression of genes encoding cell surface proteins in lactobacillus acidophilus
topic Microbiology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5387067/
https://www.ncbi.nlm.nih.gov/pubmed/28443071
http://dx.doi.org/10.3389/fmicb.2017.00553
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