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Inbred Mouse Populations Exhibit Intergenerational Changes in Intestinal Microbiota Composition and Function Following Introduction to a Facility

Inbred mice are used to investigate many aspects of human physiology, including susceptibility to disease and response to therapies. Despite increasing evidence that the composition and function of the murine intestinal microbiota can substantially influence a broad range of experimental outcomes, r...

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Autores principales: Choo, Jocelyn M., Trim, Paul J., Leong, Lex E. X., Abell, Guy C. J., Brune, Carly, Jeffries, Nicole, Wesselingh, Steve, Dear, T. N., Snel, Marten F., Rogers, Geraint B.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5387074/
https://www.ncbi.nlm.nih.gov/pubmed/28443082
http://dx.doi.org/10.3389/fmicb.2017.00608
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author Choo, Jocelyn M.
Trim, Paul J.
Leong, Lex E. X.
Abell, Guy C. J.
Brune, Carly
Jeffries, Nicole
Wesselingh, Steve
Dear, T. N.
Snel, Marten F.
Rogers, Geraint B.
author_facet Choo, Jocelyn M.
Trim, Paul J.
Leong, Lex E. X.
Abell, Guy C. J.
Brune, Carly
Jeffries, Nicole
Wesselingh, Steve
Dear, T. N.
Snel, Marten F.
Rogers, Geraint B.
author_sort Choo, Jocelyn M.
collection PubMed
description Inbred mice are used to investigate many aspects of human physiology, including susceptibility to disease and response to therapies. Despite increasing evidence that the composition and function of the murine intestinal microbiota can substantially influence a broad range of experimental outcomes, relatively little is known about microbiome dynamics within experimental mouse populations. We investigated changes in the intestinal microbiome between C57BL/6J mice spanning six generations (assessed at generations 1, 2, 3, and 6), following their introduction to a stringently controlled facility. Fecal microbiota composition and function were assessed by 16S rRNA gene amplicon sequencing and liquid chromatography mass spectrometry, respectively. Significant divergence of the intestinal microbiota between founder and second generation mice, as well as continuing inter-generational variance, was observed. Bacterial taxa whose relative abundance changed significantly through time included Akkermansia, Turicibacter, and Bifidobacterium (p < 0.05), all of which are recognized as having the potential to substantially influence host physiology. Shifts in microbiota composition were mirrored by corresponding differences in the fecal metabolome (r = 0.57, p = 0.0001), with notable differences in levels of tryptophan pathway metabolites and amino acids, including glutamine, glutamate and aspartate. We related the magnitude of changes in the intestinal microbiota and metabolome characteristics during acclimation to those observed between populations housed in separate facilities, which differed in regards to husbandry, barrier conditions and dietary intake. The microbiome variance reported here has implications for experimental reproducibility, and as a consequence, experimental design and the interpretation of research outcomes across wide range of contexts.
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spelling pubmed-53870742017-04-25 Inbred Mouse Populations Exhibit Intergenerational Changes in Intestinal Microbiota Composition and Function Following Introduction to a Facility Choo, Jocelyn M. Trim, Paul J. Leong, Lex E. X. Abell, Guy C. J. Brune, Carly Jeffries, Nicole Wesselingh, Steve Dear, T. N. Snel, Marten F. Rogers, Geraint B. Front Microbiol Microbiology Inbred mice are used to investigate many aspects of human physiology, including susceptibility to disease and response to therapies. Despite increasing evidence that the composition and function of the murine intestinal microbiota can substantially influence a broad range of experimental outcomes, relatively little is known about microbiome dynamics within experimental mouse populations. We investigated changes in the intestinal microbiome between C57BL/6J mice spanning six generations (assessed at generations 1, 2, 3, and 6), following their introduction to a stringently controlled facility. Fecal microbiota composition and function were assessed by 16S rRNA gene amplicon sequencing and liquid chromatography mass spectrometry, respectively. Significant divergence of the intestinal microbiota between founder and second generation mice, as well as continuing inter-generational variance, was observed. Bacterial taxa whose relative abundance changed significantly through time included Akkermansia, Turicibacter, and Bifidobacterium (p < 0.05), all of which are recognized as having the potential to substantially influence host physiology. Shifts in microbiota composition were mirrored by corresponding differences in the fecal metabolome (r = 0.57, p = 0.0001), with notable differences in levels of tryptophan pathway metabolites and amino acids, including glutamine, glutamate and aspartate. We related the magnitude of changes in the intestinal microbiota and metabolome characteristics during acclimation to those observed between populations housed in separate facilities, which differed in regards to husbandry, barrier conditions and dietary intake. The microbiome variance reported here has implications for experimental reproducibility, and as a consequence, experimental design and the interpretation of research outcomes across wide range of contexts. Frontiers Media S.A. 2017-04-11 /pmc/articles/PMC5387074/ /pubmed/28443082 http://dx.doi.org/10.3389/fmicb.2017.00608 Text en Copyright © 2017 Choo, Trim, Leong, Abell, Brune, Jeffries, Wesselingh, Dear, Snel and Rogers. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Microbiology
Choo, Jocelyn M.
Trim, Paul J.
Leong, Lex E. X.
Abell, Guy C. J.
Brune, Carly
Jeffries, Nicole
Wesselingh, Steve
Dear, T. N.
Snel, Marten F.
Rogers, Geraint B.
Inbred Mouse Populations Exhibit Intergenerational Changes in Intestinal Microbiota Composition and Function Following Introduction to a Facility
title Inbred Mouse Populations Exhibit Intergenerational Changes in Intestinal Microbiota Composition and Function Following Introduction to a Facility
title_full Inbred Mouse Populations Exhibit Intergenerational Changes in Intestinal Microbiota Composition and Function Following Introduction to a Facility
title_fullStr Inbred Mouse Populations Exhibit Intergenerational Changes in Intestinal Microbiota Composition and Function Following Introduction to a Facility
title_full_unstemmed Inbred Mouse Populations Exhibit Intergenerational Changes in Intestinal Microbiota Composition and Function Following Introduction to a Facility
title_short Inbred Mouse Populations Exhibit Intergenerational Changes in Intestinal Microbiota Composition and Function Following Introduction to a Facility
title_sort inbred mouse populations exhibit intergenerational changes in intestinal microbiota composition and function following introduction to a facility
topic Microbiology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5387074/
https://www.ncbi.nlm.nih.gov/pubmed/28443082
http://dx.doi.org/10.3389/fmicb.2017.00608
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