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Genetically assembled fluorescent biosensor for in situ detection of bio-synthesized alkanes
Construction of highly efficient microbial cell factories producing drop-in biofuel alkanes is severely limited due to the lack of a fast detection method against alkanes. Here we first developed a sensitive fluorescent biosensor for rapid and in situ monitoring of intracellular alkane synthesis. Us...
Autores principales: | , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Nature Publishing Group
2015
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5387116/ https://www.ncbi.nlm.nih.gov/pubmed/26039923 http://dx.doi.org/10.1038/srep10907 |
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author | Wu, Wei Zhang, Lei Yao, Lun Tan, Xiaoming Liu, Xufeng Lu, Xuefeng |
author_facet | Wu, Wei Zhang, Lei Yao, Lun Tan, Xiaoming Liu, Xufeng Lu, Xuefeng |
author_sort | Wu, Wei |
collection | PubMed |
description | Construction of highly efficient microbial cell factories producing drop-in biofuel alkanes is severely limited due to the lack of a fast detection method against alkanes. Here we first developed a sensitive fluorescent biosensor for rapid and in situ monitoring of intracellular alkane synthesis. Using GFP as reporter, the biosensor could actively respond to the intracellular alkane products, especially for the mid- and long-chain alkanes synthesized in the recombinant Escherichia coli and give a concentration-dependent fluorescence response. Our results also suggested the feasibility of developing high-throughput strategies basing on the alkane biosensor device in E. coli, and thus will greatly facilitate the application of directed evolution strategies to further improve the alkane-producing microbial cell factories. |
format | Online Article Text |
id | pubmed-5387116 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2015 |
publisher | Nature Publishing Group |
record_format | MEDLINE/PubMed |
spelling | pubmed-53871162017-04-14 Genetically assembled fluorescent biosensor for in situ detection of bio-synthesized alkanes Wu, Wei Zhang, Lei Yao, Lun Tan, Xiaoming Liu, Xufeng Lu, Xuefeng Sci Rep Article Construction of highly efficient microbial cell factories producing drop-in biofuel alkanes is severely limited due to the lack of a fast detection method against alkanes. Here we first developed a sensitive fluorescent biosensor for rapid and in situ monitoring of intracellular alkane synthesis. Using GFP as reporter, the biosensor could actively respond to the intracellular alkane products, especially for the mid- and long-chain alkanes synthesized in the recombinant Escherichia coli and give a concentration-dependent fluorescence response. Our results also suggested the feasibility of developing high-throughput strategies basing on the alkane biosensor device in E. coli, and thus will greatly facilitate the application of directed evolution strategies to further improve the alkane-producing microbial cell factories. Nature Publishing Group 2015-06-03 /pmc/articles/PMC5387116/ /pubmed/26039923 http://dx.doi.org/10.1038/srep10907 Text en Copyright © 2015, Macmillan Publishers Limited http://creativecommons.org/licenses/by/4.0/ This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/ |
spellingShingle | Article Wu, Wei Zhang, Lei Yao, Lun Tan, Xiaoming Liu, Xufeng Lu, Xuefeng Genetically assembled fluorescent biosensor for in situ detection of bio-synthesized alkanes |
title | Genetically assembled fluorescent biosensor for in situ detection of bio-synthesized alkanes |
title_full | Genetically assembled fluorescent biosensor for in situ detection of bio-synthesized alkanes |
title_fullStr | Genetically assembled fluorescent biosensor for in situ detection of bio-synthesized alkanes |
title_full_unstemmed | Genetically assembled fluorescent biosensor for in situ detection of bio-synthesized alkanes |
title_short | Genetically assembled fluorescent biosensor for in situ detection of bio-synthesized alkanes |
title_sort | genetically assembled fluorescent biosensor for in situ detection of bio-synthesized alkanes |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5387116/ https://www.ncbi.nlm.nih.gov/pubmed/26039923 http://dx.doi.org/10.1038/srep10907 |
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