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Genetically assembled fluorescent biosensor for in situ detection of bio-synthesized alkanes

Construction of highly efficient microbial cell factories producing drop-in biofuel alkanes is severely limited due to the lack of a fast detection method against alkanes. Here we first developed a sensitive fluorescent biosensor for rapid and in situ monitoring of intracellular alkane synthesis. Us...

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Detalles Bibliográficos
Autores principales: Wu, Wei, Zhang, Lei, Yao, Lun, Tan, Xiaoming, Liu, Xufeng, Lu, Xuefeng
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group 2015
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5387116/
https://www.ncbi.nlm.nih.gov/pubmed/26039923
http://dx.doi.org/10.1038/srep10907
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author Wu, Wei
Zhang, Lei
Yao, Lun
Tan, Xiaoming
Liu, Xufeng
Lu, Xuefeng
author_facet Wu, Wei
Zhang, Lei
Yao, Lun
Tan, Xiaoming
Liu, Xufeng
Lu, Xuefeng
author_sort Wu, Wei
collection PubMed
description Construction of highly efficient microbial cell factories producing drop-in biofuel alkanes is severely limited due to the lack of a fast detection method against alkanes. Here we first developed a sensitive fluorescent biosensor for rapid and in situ monitoring of intracellular alkane synthesis. Using GFP as reporter, the biosensor could actively respond to the intracellular alkane products, especially for the mid- and long-chain alkanes synthesized in the recombinant Escherichia coli and give a concentration-dependent fluorescence response. Our results also suggested the feasibility of developing high-throughput strategies basing on the alkane biosensor device in E. coli, and thus will greatly facilitate the application of directed evolution strategies to further improve the alkane-producing microbial cell factories.
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spelling pubmed-53871162017-04-14 Genetically assembled fluorescent biosensor for in situ detection of bio-synthesized alkanes Wu, Wei Zhang, Lei Yao, Lun Tan, Xiaoming Liu, Xufeng Lu, Xuefeng Sci Rep Article Construction of highly efficient microbial cell factories producing drop-in biofuel alkanes is severely limited due to the lack of a fast detection method against alkanes. Here we first developed a sensitive fluorescent biosensor for rapid and in situ monitoring of intracellular alkane synthesis. Using GFP as reporter, the biosensor could actively respond to the intracellular alkane products, especially for the mid- and long-chain alkanes synthesized in the recombinant Escherichia coli and give a concentration-dependent fluorescence response. Our results also suggested the feasibility of developing high-throughput strategies basing on the alkane biosensor device in E. coli, and thus will greatly facilitate the application of directed evolution strategies to further improve the alkane-producing microbial cell factories. Nature Publishing Group 2015-06-03 /pmc/articles/PMC5387116/ /pubmed/26039923 http://dx.doi.org/10.1038/srep10907 Text en Copyright © 2015, Macmillan Publishers Limited http://creativecommons.org/licenses/by/4.0/ This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/
spellingShingle Article
Wu, Wei
Zhang, Lei
Yao, Lun
Tan, Xiaoming
Liu, Xufeng
Lu, Xuefeng
Genetically assembled fluorescent biosensor for in situ detection of bio-synthesized alkanes
title Genetically assembled fluorescent biosensor for in situ detection of bio-synthesized alkanes
title_full Genetically assembled fluorescent biosensor for in situ detection of bio-synthesized alkanes
title_fullStr Genetically assembled fluorescent biosensor for in situ detection of bio-synthesized alkanes
title_full_unstemmed Genetically assembled fluorescent biosensor for in situ detection of bio-synthesized alkanes
title_short Genetically assembled fluorescent biosensor for in situ detection of bio-synthesized alkanes
title_sort genetically assembled fluorescent biosensor for in situ detection of bio-synthesized alkanes
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5387116/
https://www.ncbi.nlm.nih.gov/pubmed/26039923
http://dx.doi.org/10.1038/srep10907
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