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Target organs for lymphocystis disease virus replication in gilthead seabream (Sparus aurata)
The lymphocystis disease (LCD), the main viral pathology described in cultured gilthead seabream (Sparus aurata), is a self-limiting condition characterized by the appearance of hypertrophied fibroblasts (named lymphocysts) in the connective tissue of fish, primarily in the skin and fins. The causat...
Autores principales: | , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
BioMed Central
2017
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5387237/ https://www.ncbi.nlm.nih.gov/pubmed/28399906 http://dx.doi.org/10.1186/s13567-017-0428-3 |
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author | Valverde, Estefania J. Borrego, Juan J. Sarasquete, M. Carmen Ortiz-Delgado, Juan B. Castro, Dolores |
author_facet | Valverde, Estefania J. Borrego, Juan J. Sarasquete, M. Carmen Ortiz-Delgado, Juan B. Castro, Dolores |
author_sort | Valverde, Estefania J. |
collection | PubMed |
description | The lymphocystis disease (LCD), the main viral pathology described in cultured gilthead seabream (Sparus aurata), is a self-limiting condition characterized by the appearance of hypertrophied fibroblasts (named lymphocysts) in the connective tissue of fish, primarily in the skin and fins. The causative agent of the disease is the Lymphocystis disease virus (LCDV), a member of the Iridoviridae family. In the present study, LCDV genome and transcripts were detected by real-time PCR in caudal fin, as well as in several internal organs, such as intestine, liver, spleen, kidney and brain, from asymptomatic, diseased and recovered gilthead seabream juveniles. These results indicate that the LCDV has a broad range tissue tropism, and can establish a systemic infection, even in subclinically infected fish. As showed by in situ hybridization, the permissive cells for LCDV infection seem to be fibroblasts, hepatocytes and cells of the mononuclear phagocyte system. Histopathological alterations associated with LCD were observed in all the organs analysed, including necrotic changes in liver and kidney, inflammatory response in the intestine submucosa or brain haemorrhage, although lymphocysts were only detected in the dermis of the caudal fin. Nevertheless, these histological changes were reverted in recovered animals. |
format | Online Article Text |
id | pubmed-5387237 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2017 |
publisher | BioMed Central |
record_format | MEDLINE/PubMed |
spelling | pubmed-53872372017-04-11 Target organs for lymphocystis disease virus replication in gilthead seabream (Sparus aurata) Valverde, Estefania J. Borrego, Juan J. Sarasquete, M. Carmen Ortiz-Delgado, Juan B. Castro, Dolores Vet Res Research Article The lymphocystis disease (LCD), the main viral pathology described in cultured gilthead seabream (Sparus aurata), is a self-limiting condition characterized by the appearance of hypertrophied fibroblasts (named lymphocysts) in the connective tissue of fish, primarily in the skin and fins. The causative agent of the disease is the Lymphocystis disease virus (LCDV), a member of the Iridoviridae family. In the present study, LCDV genome and transcripts were detected by real-time PCR in caudal fin, as well as in several internal organs, such as intestine, liver, spleen, kidney and brain, from asymptomatic, diseased and recovered gilthead seabream juveniles. These results indicate that the LCDV has a broad range tissue tropism, and can establish a systemic infection, even in subclinically infected fish. As showed by in situ hybridization, the permissive cells for LCDV infection seem to be fibroblasts, hepatocytes and cells of the mononuclear phagocyte system. Histopathological alterations associated with LCD were observed in all the organs analysed, including necrotic changes in liver and kidney, inflammatory response in the intestine submucosa or brain haemorrhage, although lymphocysts were only detected in the dermis of the caudal fin. Nevertheless, these histological changes were reverted in recovered animals. BioMed Central 2017-04-11 2017 /pmc/articles/PMC5387237/ /pubmed/28399906 http://dx.doi.org/10.1186/s13567-017-0428-3 Text en © The Author(s) 2017 Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated. |
spellingShingle | Research Article Valverde, Estefania J. Borrego, Juan J. Sarasquete, M. Carmen Ortiz-Delgado, Juan B. Castro, Dolores Target organs for lymphocystis disease virus replication in gilthead seabream (Sparus aurata) |
title | Target organs for lymphocystis disease virus replication in gilthead seabream (Sparus aurata) |
title_full | Target organs for lymphocystis disease virus replication in gilthead seabream (Sparus aurata) |
title_fullStr | Target organs for lymphocystis disease virus replication in gilthead seabream (Sparus aurata) |
title_full_unstemmed | Target organs for lymphocystis disease virus replication in gilthead seabream (Sparus aurata) |
title_short | Target organs for lymphocystis disease virus replication in gilthead seabream (Sparus aurata) |
title_sort | target organs for lymphocystis disease virus replication in gilthead seabream (sparus aurata) |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5387237/ https://www.ncbi.nlm.nih.gov/pubmed/28399906 http://dx.doi.org/10.1186/s13567-017-0428-3 |
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