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Prevalence of Salmonella in poultry processing environments in wet markets in Penang and Perlis, Malaysia

AIM: The aim of this study was to determine the prevalence of various Salmonella serotypes in chickens, carcass contact surfaces as well as environmental samples collected from wet markets and small scale processing plant. MATERIALS AND METHODS: A total of 182 poultry and environmental samples were...

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Autores principales: Nidaullah, Hafiz, Abirami, Nadarajan, Shamila-Syuhada, Ahamed Kamal, Chuah, Li-Oon, Nurul, Huda, Tan, Teik Pei, Abidin, Farah Wahida Zainal, Rusul, Gulam
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Veterinary World 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5387655/
https://www.ncbi.nlm.nih.gov/pubmed/28435190
http://dx.doi.org/10.14202/vetworld.2017.286-292
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author Nidaullah, Hafiz
Abirami, Nadarajan
Shamila-Syuhada, Ahamed Kamal
Chuah, Li-Oon
Nurul, Huda
Tan, Teik Pei
Abidin, Farah Wahida Zainal
Rusul, Gulam
author_facet Nidaullah, Hafiz
Abirami, Nadarajan
Shamila-Syuhada, Ahamed Kamal
Chuah, Li-Oon
Nurul, Huda
Tan, Teik Pei
Abidin, Farah Wahida Zainal
Rusul, Gulam
author_sort Nidaullah, Hafiz
collection PubMed
description AIM: The aim of this study was to determine the prevalence of various Salmonella serotypes in chickens, carcass contact surfaces as well as environmental samples collected from wet markets and small scale processing plant. MATERIALS AND METHODS: A total of 182 poultry and environmental samples were collected at random on separate occasions from wet markets and small scale processing plant, during the period of October 2014 to July 2015 in Penang and Perlis, Malaysia. The samples were analyzed for the presence of Salmonella using ISO 6579:2002 conventional culture-based method. Presumptive Salmonella colonies were subjected to various biochemical tests (such as triple sugar iron and lysine iron test), serologically confirmed using polyvalent O and H antisera and further serotyped at Public Health Laboratory, Ministry of Health, Perak, Malaysia. RESULTS: Salmonella serotypes were isolated from 161 out of 182 samples (88.46%) with 100% prevalence in the whole chicken carcass and chicken cuts - as well as transport crate, cage, drum, knife, chopping board, display table, floor, bench wash water, wash water, and drain water. Salmonella was isolated from 91.67%, 83.33%, and 66.67% of defeathering machines, drain swabs, and apron, respectively. 17 serotypes were isolated in this study with Salmonella Albany (57/161), Salmonella Corvallis (42/161), and Salmonella Brancaster (37/161) being the predominant serovars. CONCLUSION: The most carcass contact and environmental samples collected along the wet market chicken processing line were consistently contaminated with Salmonella. This indicates that Salmonella has established itself in poultry processing environments by colonizing the surfaces of the equipment and survives in these environments by establishing biofilms. Our results highlight the need of implementing strict hygiene and sanitation standards to reduce the incidence of Salmonella. The prevalence of Salmonella in poultry can be reduced effectively by identifying and eliminating the sources and contamination sites during slaughter and processing of poultry.
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spelling pubmed-53876552017-04-21 Prevalence of Salmonella in poultry processing environments in wet markets in Penang and Perlis, Malaysia Nidaullah, Hafiz Abirami, Nadarajan Shamila-Syuhada, Ahamed Kamal Chuah, Li-Oon Nurul, Huda Tan, Teik Pei Abidin, Farah Wahida Zainal Rusul, Gulam Vet World Research Article AIM: The aim of this study was to determine the prevalence of various Salmonella serotypes in chickens, carcass contact surfaces as well as environmental samples collected from wet markets and small scale processing plant. MATERIALS AND METHODS: A total of 182 poultry and environmental samples were collected at random on separate occasions from wet markets and small scale processing plant, during the period of October 2014 to July 2015 in Penang and Perlis, Malaysia. The samples were analyzed for the presence of Salmonella using ISO 6579:2002 conventional culture-based method. Presumptive Salmonella colonies were subjected to various biochemical tests (such as triple sugar iron and lysine iron test), serologically confirmed using polyvalent O and H antisera and further serotyped at Public Health Laboratory, Ministry of Health, Perak, Malaysia. RESULTS: Salmonella serotypes were isolated from 161 out of 182 samples (88.46%) with 100% prevalence in the whole chicken carcass and chicken cuts - as well as transport crate, cage, drum, knife, chopping board, display table, floor, bench wash water, wash water, and drain water. Salmonella was isolated from 91.67%, 83.33%, and 66.67% of defeathering machines, drain swabs, and apron, respectively. 17 serotypes were isolated in this study with Salmonella Albany (57/161), Salmonella Corvallis (42/161), and Salmonella Brancaster (37/161) being the predominant serovars. CONCLUSION: The most carcass contact and environmental samples collected along the wet market chicken processing line were consistently contaminated with Salmonella. This indicates that Salmonella has established itself in poultry processing environments by colonizing the surfaces of the equipment and survives in these environments by establishing biofilms. Our results highlight the need of implementing strict hygiene and sanitation standards to reduce the incidence of Salmonella. The prevalence of Salmonella in poultry can be reduced effectively by identifying and eliminating the sources and contamination sites during slaughter and processing of poultry. Veterinary World 2017-03 2017-03-06 /pmc/articles/PMC5387655/ /pubmed/28435190 http://dx.doi.org/10.14202/vetworld.2017.286-292 Text en Copyright: © Nidaullah, et al. http://creativecommons.org/licenses/by/4.0 Open Access. This article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.
spellingShingle Research Article
Nidaullah, Hafiz
Abirami, Nadarajan
Shamila-Syuhada, Ahamed Kamal
Chuah, Li-Oon
Nurul, Huda
Tan, Teik Pei
Abidin, Farah Wahida Zainal
Rusul, Gulam
Prevalence of Salmonella in poultry processing environments in wet markets in Penang and Perlis, Malaysia
title Prevalence of Salmonella in poultry processing environments in wet markets in Penang and Perlis, Malaysia
title_full Prevalence of Salmonella in poultry processing environments in wet markets in Penang and Perlis, Malaysia
title_fullStr Prevalence of Salmonella in poultry processing environments in wet markets in Penang and Perlis, Malaysia
title_full_unstemmed Prevalence of Salmonella in poultry processing environments in wet markets in Penang and Perlis, Malaysia
title_short Prevalence of Salmonella in poultry processing environments in wet markets in Penang and Perlis, Malaysia
title_sort prevalence of salmonella in poultry processing environments in wet markets in penang and perlis, malaysia
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5387655/
https://www.ncbi.nlm.nih.gov/pubmed/28435190
http://dx.doi.org/10.14202/vetworld.2017.286-292
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