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Screening for antibacterial and antibiofilm activity in Thai medicinal plant extracts against oral microorganisms

To evaluate the antibacterial activity of 12 ethanol extracts of Thai traditional herb against oral pathogens. The antibacterial activities were assessed by agar well diffusion, broth microdilution, and time-kill methods. Antibiofilm activity was investigated using a 3-[4,5-dimethyl-2-thiazolyl]-2,5...

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Autores principales: Teanpaisan, Rawee, Kawsud, Pajaree, Pahumunto, Nuntiya, Puripattanavong, Jindaporn
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2016
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5388082/
https://www.ncbi.nlm.nih.gov/pubmed/28417087
http://dx.doi.org/10.1016/j.jtcme.2016.06.007
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author Teanpaisan, Rawee
Kawsud, Pajaree
Pahumunto, Nuntiya
Puripattanavong, Jindaporn
author_facet Teanpaisan, Rawee
Kawsud, Pajaree
Pahumunto, Nuntiya
Puripattanavong, Jindaporn
author_sort Teanpaisan, Rawee
collection PubMed
description To evaluate the antibacterial activity of 12 ethanol extracts of Thai traditional herb against oral pathogens. The antibacterial activities were assessed by agar well diffusion, broth microdilution, and time-kill methods. Antibiofilm activity was investigated using a 3-[4,5-dimethyl-2-thiazolyl]-2,5-diphenyl-2H-tetrazolium-bromide (MTT) assay. High performance liquid chromatography (HPLC), thin layer chromatography (TLC) fingerprinting, and TLC-bioautography were used to determine the active antibacterial compounds. Piper betle showed the best antibacterial activities against all tested strains in the minimal inhibitory concentration and minimal bactericidal concentration, ranged from 1.04–5.21 mg/mL and 2.08–8.33 mg/mL, respectively. Killing ability depended on time and concentrations of the extract. P. betle extract acts as a potent antibiofilm agent with dual actions, preventing and eradicating the biofilm. The major constituent of P. betle extract was 4-chromanol, which responded for antibacteria and antibiofilm against oral pathogens. It suggests that the ethanol P. betle leaves extract may be used for preventing oral diseases.
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spelling pubmed-53880822017-04-17 Screening for antibacterial and antibiofilm activity in Thai medicinal plant extracts against oral microorganisms Teanpaisan, Rawee Kawsud, Pajaree Pahumunto, Nuntiya Puripattanavong, Jindaporn J Tradit Complement Med Original Article To evaluate the antibacterial activity of 12 ethanol extracts of Thai traditional herb against oral pathogens. The antibacterial activities were assessed by agar well diffusion, broth microdilution, and time-kill methods. Antibiofilm activity was investigated using a 3-[4,5-dimethyl-2-thiazolyl]-2,5-diphenyl-2H-tetrazolium-bromide (MTT) assay. High performance liquid chromatography (HPLC), thin layer chromatography (TLC) fingerprinting, and TLC-bioautography were used to determine the active antibacterial compounds. Piper betle showed the best antibacterial activities against all tested strains in the minimal inhibitory concentration and minimal bactericidal concentration, ranged from 1.04–5.21 mg/mL and 2.08–8.33 mg/mL, respectively. Killing ability depended on time and concentrations of the extract. P. betle extract acts as a potent antibiofilm agent with dual actions, preventing and eradicating the biofilm. The major constituent of P. betle extract was 4-chromanol, which responded for antibacteria and antibiofilm against oral pathogens. It suggests that the ethanol P. betle leaves extract may be used for preventing oral diseases. Elsevier 2016-07-12 /pmc/articles/PMC5388082/ /pubmed/28417087 http://dx.doi.org/10.1016/j.jtcme.2016.06.007 Text en Copyright © 2016, Center for Food and Biomolecules, National Taiwan University. Production and hosting by Elsevier Taiwan LLC. http://creativecommons.org/licenses/by-nc-nd/4.0/ This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
spellingShingle Original Article
Teanpaisan, Rawee
Kawsud, Pajaree
Pahumunto, Nuntiya
Puripattanavong, Jindaporn
Screening for antibacterial and antibiofilm activity in Thai medicinal plant extracts against oral microorganisms
title Screening for antibacterial and antibiofilm activity in Thai medicinal plant extracts against oral microorganisms
title_full Screening for antibacterial and antibiofilm activity in Thai medicinal plant extracts against oral microorganisms
title_fullStr Screening for antibacterial and antibiofilm activity in Thai medicinal plant extracts against oral microorganisms
title_full_unstemmed Screening for antibacterial and antibiofilm activity in Thai medicinal plant extracts against oral microorganisms
title_short Screening for antibacterial and antibiofilm activity in Thai medicinal plant extracts against oral microorganisms
title_sort screening for antibacterial and antibiofilm activity in thai medicinal plant extracts against oral microorganisms
topic Original Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5388082/
https://www.ncbi.nlm.nih.gov/pubmed/28417087
http://dx.doi.org/10.1016/j.jtcme.2016.06.007
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