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Novel real-time PCR-based patho- and phylotyping of potentially zoonotic avian influenza A subtype H5 viruses at risk of incursion into Europe in 2017

Since November 2016, Europe witnesses another wave of incursion of highly pathogenic avian influenza (HPAI) A(H5) viruses of the Asian origin goose/Guangdong (gs/GD) lineage. Infections with H5 viruses of clade 2.3.4.4b affect wild bird and poultry populations. H5 viruses of clades 2.2, 2.3.1.2c and...

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Autores principales: Naguib, Mahmoud M, Graaf, Annika, Fortin, Andrea, Luttermann, Christine, Wernery, Ulrich, Amarin, Nadim, Hussein, Hussein A, Sultan, Hesham, Al Adhadh, Basem, Hassan, Mohamed K, Beer, Martin, Monne, Isabella, Harder, Timm C
Formato: Online Artículo Texto
Lenguaje:English
Publicado: European Centre for Disease Prevention and Control (ECDC) 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5388100/
https://www.ncbi.nlm.nih.gov/pubmed/28084214
http://dx.doi.org/10.2807/1560-7917.ES.2017.22.1.30435
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author Naguib, Mahmoud M
Graaf, Annika
Fortin, Andrea
Luttermann, Christine
Wernery, Ulrich
Amarin, Nadim
Hussein, Hussein A
Sultan, Hesham
Al Adhadh, Basem
Hassan, Mohamed K
Beer, Martin
Monne, Isabella
Harder, Timm C
author_facet Naguib, Mahmoud M
Graaf, Annika
Fortin, Andrea
Luttermann, Christine
Wernery, Ulrich
Amarin, Nadim
Hussein, Hussein A
Sultan, Hesham
Al Adhadh, Basem
Hassan, Mohamed K
Beer, Martin
Monne, Isabella
Harder, Timm C
author_sort Naguib, Mahmoud M
collection PubMed
description Since November 2016, Europe witnesses another wave of incursion of highly pathogenic avian influenza (HPAI) A(H5) viruses of the Asian origin goose/Guangdong (gs/GD) lineage. Infections with H5 viruses of clade 2.3.4.4b affect wild bird and poultry populations. H5 viruses of clades 2.2, 2.3.1.2c and 2.3.4.4a were detected previously in Europe in 2006, 2010 and 2014. Clades 2.2.1.2 and 2.3.2.1.c are endemic in Egypt and Western Africa, respectively and have caused human fatalities. Evidence exists of their co-circulation in the Middle East. Subtype H5 viruses of low pathogenicity (LPAI) are endemic in migratory wild bird populations. They potentially mutate into highly pathogenic phenotypes following transmission into poultry holdings. However, to date only the gs/GD H5 lineage had an impact on human health. Rapid and specific diagnosis marks the cornerstone for control and eradication of HPAI virus incursions. We present the development and validation of five real-time RT-PCR assays (RT-qPCR) that allow sequencing-independent pathotype and clade-specific distinction of major gs/GD HPAI H5 virus clades and of Eurasian LPAI viruses currently circulating. Together with an influenza A virus-generic RT-qPCR, the assays significantly speed up time-to-diagnosis and reduce reaction times in a OneHealth approach of curbing the spread of gs/GD HPAI viruses.
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spelling pubmed-53881002017-04-24 Novel real-time PCR-based patho- and phylotyping of potentially zoonotic avian influenza A subtype H5 viruses at risk of incursion into Europe in 2017 Naguib, Mahmoud M Graaf, Annika Fortin, Andrea Luttermann, Christine Wernery, Ulrich Amarin, Nadim Hussein, Hussein A Sultan, Hesham Al Adhadh, Basem Hassan, Mohamed K Beer, Martin Monne, Isabella Harder, Timm C Euro Surveill Research Article Since November 2016, Europe witnesses another wave of incursion of highly pathogenic avian influenza (HPAI) A(H5) viruses of the Asian origin goose/Guangdong (gs/GD) lineage. Infections with H5 viruses of clade 2.3.4.4b affect wild bird and poultry populations. H5 viruses of clades 2.2, 2.3.1.2c and 2.3.4.4a were detected previously in Europe in 2006, 2010 and 2014. Clades 2.2.1.2 and 2.3.2.1.c are endemic in Egypt and Western Africa, respectively and have caused human fatalities. Evidence exists of their co-circulation in the Middle East. Subtype H5 viruses of low pathogenicity (LPAI) are endemic in migratory wild bird populations. They potentially mutate into highly pathogenic phenotypes following transmission into poultry holdings. However, to date only the gs/GD H5 lineage had an impact on human health. Rapid and specific diagnosis marks the cornerstone for control and eradication of HPAI virus incursions. We present the development and validation of five real-time RT-PCR assays (RT-qPCR) that allow sequencing-independent pathotype and clade-specific distinction of major gs/GD HPAI H5 virus clades and of Eurasian LPAI viruses currently circulating. Together with an influenza A virus-generic RT-qPCR, the assays significantly speed up time-to-diagnosis and reduce reaction times in a OneHealth approach of curbing the spread of gs/GD HPAI viruses. European Centre for Disease Prevention and Control (ECDC) 2017-01-05 /pmc/articles/PMC5388100/ /pubmed/28084214 http://dx.doi.org/10.2807/1560-7917.ES.2017.22.1.30435 Text en This article is copyright of The Authors, 2017. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution (CC BY 4.0) Licence. You may share and adapt the material, but must give appropriate credit to the source, provide a link to the licence, and indicate if changes were made.
spellingShingle Research Article
Naguib, Mahmoud M
Graaf, Annika
Fortin, Andrea
Luttermann, Christine
Wernery, Ulrich
Amarin, Nadim
Hussein, Hussein A
Sultan, Hesham
Al Adhadh, Basem
Hassan, Mohamed K
Beer, Martin
Monne, Isabella
Harder, Timm C
Novel real-time PCR-based patho- and phylotyping of potentially zoonotic avian influenza A subtype H5 viruses at risk of incursion into Europe in 2017
title Novel real-time PCR-based patho- and phylotyping of potentially zoonotic avian influenza A subtype H5 viruses at risk of incursion into Europe in 2017
title_full Novel real-time PCR-based patho- and phylotyping of potentially zoonotic avian influenza A subtype H5 viruses at risk of incursion into Europe in 2017
title_fullStr Novel real-time PCR-based patho- and phylotyping of potentially zoonotic avian influenza A subtype H5 viruses at risk of incursion into Europe in 2017
title_full_unstemmed Novel real-time PCR-based patho- and phylotyping of potentially zoonotic avian influenza A subtype H5 viruses at risk of incursion into Europe in 2017
title_short Novel real-time PCR-based patho- and phylotyping of potentially zoonotic avian influenza A subtype H5 viruses at risk of incursion into Europe in 2017
title_sort novel real-time pcr-based patho- and phylotyping of potentially zoonotic avian influenza a subtype h5 viruses at risk of incursion into europe in 2017
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5388100/
https://www.ncbi.nlm.nih.gov/pubmed/28084214
http://dx.doi.org/10.2807/1560-7917.ES.2017.22.1.30435
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