In Vitro Comparative Skin Irritation Induced by Nano and Non-Nano Zinc Oxide

This study was designed to determine whether nano-sized ZnO has the potential to cause acute cutaneous irritation using cultured HaCaT keratinocytes and a human skin equivalent as in vitro models, compared to non-nanomaterials. Commercial nano ZnO with different sizes (50 nm and 100 nm) was characte...

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Autores principales: Vinardell, Maria Pilar, Llanas, Hector, Marics, Laura, Mitjans, Montserrat
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2017
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5388158/
https://www.ncbi.nlm.nih.gov/pubmed/28336890
http://dx.doi.org/10.3390/nano7030056
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author Vinardell, Maria Pilar
Llanas, Hector
Marics, Laura
Mitjans, Montserrat
author_facet Vinardell, Maria Pilar
Llanas, Hector
Marics, Laura
Mitjans, Montserrat
author_sort Vinardell, Maria Pilar
collection PubMed
description This study was designed to determine whether nano-sized ZnO has the potential to cause acute cutaneous irritation using cultured HaCaT keratinocytes and a human skin equivalent as in vitro models, compared to non-nanomaterials. Commercial nano ZnO with different sizes (50 nm and 100 nm) was characterized by dynamic light scattering (DLS) and microscopy (SEM) in different media. Nano ZnO reduced the cell viability of HaCaT in a dose-dependent and time-dependent manner, in a similar way to macro ZnO. However, the 3D-epidermis model revealed no irritation at 1 mg/mL after 24 h of exposure. In conclusion, nano-sized ZnO does not irritate skin, in a similar manner to non-nano ZnO.
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spelling pubmed-53881582017-04-13 In Vitro Comparative Skin Irritation Induced by Nano and Non-Nano Zinc Oxide Vinardell, Maria Pilar Llanas, Hector Marics, Laura Mitjans, Montserrat Nanomaterials (Basel) Article This study was designed to determine whether nano-sized ZnO has the potential to cause acute cutaneous irritation using cultured HaCaT keratinocytes and a human skin equivalent as in vitro models, compared to non-nanomaterials. Commercial nano ZnO with different sizes (50 nm and 100 nm) was characterized by dynamic light scattering (DLS) and microscopy (SEM) in different media. Nano ZnO reduced the cell viability of HaCaT in a dose-dependent and time-dependent manner, in a similar way to macro ZnO. However, the 3D-epidermis model revealed no irritation at 1 mg/mL after 24 h of exposure. In conclusion, nano-sized ZnO does not irritate skin, in a similar manner to non-nano ZnO. MDPI 2017-03-04 /pmc/articles/PMC5388158/ /pubmed/28336890 http://dx.doi.org/10.3390/nano7030056 Text en © 2017 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Vinardell, Maria Pilar
Llanas, Hector
Marics, Laura
Mitjans, Montserrat
In Vitro Comparative Skin Irritation Induced by Nano and Non-Nano Zinc Oxide
title In Vitro Comparative Skin Irritation Induced by Nano and Non-Nano Zinc Oxide
title_full In Vitro Comparative Skin Irritation Induced by Nano and Non-Nano Zinc Oxide
title_fullStr In Vitro Comparative Skin Irritation Induced by Nano and Non-Nano Zinc Oxide
title_full_unstemmed In Vitro Comparative Skin Irritation Induced by Nano and Non-Nano Zinc Oxide
title_short In Vitro Comparative Skin Irritation Induced by Nano and Non-Nano Zinc Oxide
title_sort in vitro comparative skin irritation induced by nano and non-nano zinc oxide
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5388158/
https://www.ncbi.nlm.nih.gov/pubmed/28336890
http://dx.doi.org/10.3390/nano7030056
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AT maricslaura invitrocomparativeskinirritationinducedbynanoandnonnanozincoxide
AT mitjansmontserrat invitrocomparativeskinirritationinducedbynanoandnonnanozincoxide

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