Derivation of Ethnically Diverse Human Induced Pluripotent Stem Cell Lines

The human genome with all its ethnic variations contributes to differences in human development, aging, disease, repair, and response to medical treatments and is an exciting area of research and clinical study. The availability of well-characterized ethnically diverse stem cell lines is limited and...

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Autores principales: Chang, Eun Ah, Tomov, Martin L., Suhr, Steven T., Luo, Jiesi, Olmsted, Zachary T., Paluh, Janet L., Cibelli, Jose
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group 2015
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5388175/
https://www.ncbi.nlm.nih.gov/pubmed/26482195
http://dx.doi.org/10.1038/srep15234
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author Chang, Eun Ah
Tomov, Martin L.
Suhr, Steven T.
Luo, Jiesi
Olmsted, Zachary T.
Paluh, Janet L.
Cibelli, Jose
author_facet Chang, Eun Ah
Tomov, Martin L.
Suhr, Steven T.
Luo, Jiesi
Olmsted, Zachary T.
Paluh, Janet L.
Cibelli, Jose
author_sort Chang, Eun Ah
collection PubMed
description The human genome with all its ethnic variations contributes to differences in human development, aging, disease, repair, and response to medical treatments and is an exciting area of research and clinical study. The availability of well-characterized ethnically diverse stem cell lines is limited and has not kept pace with other advances in stem cell research. Here we derived xenofree ethnically diverse-human induced pluripotent stem cell (ED-iPSC) lines from fibroblasts obtained from individuals of African American, Hispanic-Latino, Asian, and Caucasian ethnic origin and have characterized the lines under a uniform platform for comparative analysis. Derived ED-iPSC lines are low passage number and evaluated in vivo by teratoma formation and in vitro by high throughput microarray analysis of EB formation and early differentiation for tri-lineage commitment to endoderm, ectoderm and mesoderm. These new xenofree ED-iPSC lines represent a well-characterized valuable resource with potential for use in future research in drug discovery or clinical investigations.
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spelling pubmed-53881752017-04-14 Derivation of Ethnically Diverse Human Induced Pluripotent Stem Cell Lines Chang, Eun Ah Tomov, Martin L. Suhr, Steven T. Luo, Jiesi Olmsted, Zachary T. Paluh, Janet L. Cibelli, Jose Sci Rep Article The human genome with all its ethnic variations contributes to differences in human development, aging, disease, repair, and response to medical treatments and is an exciting area of research and clinical study. The availability of well-characterized ethnically diverse stem cell lines is limited and has not kept pace with other advances in stem cell research. Here we derived xenofree ethnically diverse-human induced pluripotent stem cell (ED-iPSC) lines from fibroblasts obtained from individuals of African American, Hispanic-Latino, Asian, and Caucasian ethnic origin and have characterized the lines under a uniform platform for comparative analysis. Derived ED-iPSC lines are low passage number and evaluated in vivo by teratoma formation and in vitro by high throughput microarray analysis of EB formation and early differentiation for tri-lineage commitment to endoderm, ectoderm and mesoderm. These new xenofree ED-iPSC lines represent a well-characterized valuable resource with potential for use in future research in drug discovery or clinical investigations. Nature Publishing Group 2015-10-20 /pmc/articles/PMC5388175/ /pubmed/26482195 http://dx.doi.org/10.1038/srep15234 Text en Copyright © 2015, Macmillan Publishers Limited http://creativecommons.org/licenses/by/4.0/ This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/
spellingShingle Article
Chang, Eun Ah
Tomov, Martin L.
Suhr, Steven T.
Luo, Jiesi
Olmsted, Zachary T.
Paluh, Janet L.
Cibelli, Jose
Derivation of Ethnically Diverse Human Induced Pluripotent Stem Cell Lines
title Derivation of Ethnically Diverse Human Induced Pluripotent Stem Cell Lines
title_full Derivation of Ethnically Diverse Human Induced Pluripotent Stem Cell Lines
title_fullStr Derivation of Ethnically Diverse Human Induced Pluripotent Stem Cell Lines
title_full_unstemmed Derivation of Ethnically Diverse Human Induced Pluripotent Stem Cell Lines
title_short Derivation of Ethnically Diverse Human Induced Pluripotent Stem Cell Lines
title_sort derivation of ethnically diverse human induced pluripotent stem cell lines
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5388175/
https://www.ncbi.nlm.nih.gov/pubmed/26482195
http://dx.doi.org/10.1038/srep15234
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