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Screening and Optimizing Antimicrobial Peptides by Using SPOT-Synthesis
Peptide arrays on cellulose are a powerful tool to investigate peptide interactions with a number of different molecules, for examples antibodies, receptors or enzymes. Such peptide arrays can also be used to study interactions with whole cells. In this review, we focus on the interaction of small a...
Autores principales: | , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Frontiers Media S.A.
2017
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5388751/ https://www.ncbi.nlm.nih.gov/pubmed/28447030 http://dx.doi.org/10.3389/fchem.2017.00025 |
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author | López-Pérez, Paula M. Grimsey, Elizabeth Bourne, Luc Mikut, Ralf Hilpert, Kai |
author_facet | López-Pérez, Paula M. Grimsey, Elizabeth Bourne, Luc Mikut, Ralf Hilpert, Kai |
author_sort | López-Pérez, Paula M. |
collection | PubMed |
description | Peptide arrays on cellulose are a powerful tool to investigate peptide interactions with a number of different molecules, for examples antibodies, receptors or enzymes. Such peptide arrays can also be used to study interactions with whole cells. In this review, we focus on the interaction of small antimicrobial peptides with bacteria. Antimicrobial peptides (AMPs) can kill multidrug-resistant (MDR) human pathogenic bacteria and therefore could be next generation antibiotics targeting MDR bacteria. We describe the screen and the result of different optimization strategies of peptides cleaved from the membrane. In addition, screening of antibacterial activity of peptides that are tethered to the surface is discussed. Surface-active peptides can be used to protect surfaces from bacterial infections, for example implants. |
format | Online Article Text |
id | pubmed-5388751 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2017 |
publisher | Frontiers Media S.A. |
record_format | MEDLINE/PubMed |
spelling | pubmed-53887512017-04-26 Screening and Optimizing Antimicrobial Peptides by Using SPOT-Synthesis López-Pérez, Paula M. Grimsey, Elizabeth Bourne, Luc Mikut, Ralf Hilpert, Kai Front Chem Chemistry Peptide arrays on cellulose are a powerful tool to investigate peptide interactions with a number of different molecules, for examples antibodies, receptors or enzymes. Such peptide arrays can also be used to study interactions with whole cells. In this review, we focus on the interaction of small antimicrobial peptides with bacteria. Antimicrobial peptides (AMPs) can kill multidrug-resistant (MDR) human pathogenic bacteria and therefore could be next generation antibiotics targeting MDR bacteria. We describe the screen and the result of different optimization strategies of peptides cleaved from the membrane. In addition, screening of antibacterial activity of peptides that are tethered to the surface is discussed. Surface-active peptides can be used to protect surfaces from bacterial infections, for example implants. Frontiers Media S.A. 2017-04-12 /pmc/articles/PMC5388751/ /pubmed/28447030 http://dx.doi.org/10.3389/fchem.2017.00025 Text en Copyright © 2017 López-Pérez, Grimsey, Bourne, Mikut and Hilpert. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms. |
spellingShingle | Chemistry López-Pérez, Paula M. Grimsey, Elizabeth Bourne, Luc Mikut, Ralf Hilpert, Kai Screening and Optimizing Antimicrobial Peptides by Using SPOT-Synthesis |
title | Screening and Optimizing Antimicrobial Peptides by Using SPOT-Synthesis |
title_full | Screening and Optimizing Antimicrobial Peptides by Using SPOT-Synthesis |
title_fullStr | Screening and Optimizing Antimicrobial Peptides by Using SPOT-Synthesis |
title_full_unstemmed | Screening and Optimizing Antimicrobial Peptides by Using SPOT-Synthesis |
title_short | Screening and Optimizing Antimicrobial Peptides by Using SPOT-Synthesis |
title_sort | screening and optimizing antimicrobial peptides by using spot-synthesis |
topic | Chemistry |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5388751/ https://www.ncbi.nlm.nih.gov/pubmed/28447030 http://dx.doi.org/10.3389/fchem.2017.00025 |
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