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Bone marrow mesenchymal stem cells combine with Treated dentin matrix to build biological root

Treated dentin matrix (TDM) as a kind of scaffolding material has been proved odontogenic induction ability on dental-derived stem cells. Given the limited resources of dental stem cells, it is necessary to seek new seed cell which easily obtained. Jaw bone marrow mesenchymal stem cell (JBMMSC) as n...

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Autores principales: Luo, Shiwei, Pei, Fei, Zhang, Wen, Guo, Weihua, Li, Rui, He, Wei, Tian, Weidong
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5388852/
https://www.ncbi.nlm.nih.gov/pubmed/28401887
http://dx.doi.org/10.1038/srep44635
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author Luo, Shiwei
Pei, Fei
Zhang, Wen
Guo, Weihua
Li, Rui
He, Wei
Tian, Weidong
author_facet Luo, Shiwei
Pei, Fei
Zhang, Wen
Guo, Weihua
Li, Rui
He, Wei
Tian, Weidong
author_sort Luo, Shiwei
collection PubMed
description Treated dentin matrix (TDM) as a kind of scaffolding material has been proved odontogenic induction ability on dental-derived stem cells. Given the limited resources of dental stem cells, it is necessary to seek new seed cell which easily obtained. Jaw bone marrow mesenchymal stem cell (JBMMSC) as non-dental-derived stem cell relates to the development of teeth and jaws which suggest us JBMMSCs could act as a new seed cell for tooth tissue engineering. To assess the odontogenic and osteogenic potential of JBMMSCs, cells were induced by TDM extraction in vitro and combined with TDM in vivo. Results were analyzed by PCR, Western Blotting and histology. PCR and Western Blotting showed odontogenic and osteogenic makers were significantly enhanced in varying degrees after induced by TDM extraction in vitro. In vivo, JBMMSCs expressed both odontogenic and osteogenic-related protein, and the latter showed stronger positive expression. Furthermore, histological examination of the harvested grafts was observed the formation of bone-like tissue. Therefore, osteogenic differentiation ability of JBMMSCs were enhanced significantly after being inducted by TDM which illustrates that non-odontogenic derived stem cells are still promising seed cells in tooth root tissue engineering.
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spelling pubmed-53888522017-04-14 Bone marrow mesenchymal stem cells combine with Treated dentin matrix to build biological root Luo, Shiwei Pei, Fei Zhang, Wen Guo, Weihua Li, Rui He, Wei Tian, Weidong Sci Rep Article Treated dentin matrix (TDM) as a kind of scaffolding material has been proved odontogenic induction ability on dental-derived stem cells. Given the limited resources of dental stem cells, it is necessary to seek new seed cell which easily obtained. Jaw bone marrow mesenchymal stem cell (JBMMSC) as non-dental-derived stem cell relates to the development of teeth and jaws which suggest us JBMMSCs could act as a new seed cell for tooth tissue engineering. To assess the odontogenic and osteogenic potential of JBMMSCs, cells were induced by TDM extraction in vitro and combined with TDM in vivo. Results were analyzed by PCR, Western Blotting and histology. PCR and Western Blotting showed odontogenic and osteogenic makers were significantly enhanced in varying degrees after induced by TDM extraction in vitro. In vivo, JBMMSCs expressed both odontogenic and osteogenic-related protein, and the latter showed stronger positive expression. Furthermore, histological examination of the harvested grafts was observed the formation of bone-like tissue. Therefore, osteogenic differentiation ability of JBMMSCs were enhanced significantly after being inducted by TDM which illustrates that non-odontogenic derived stem cells are still promising seed cells in tooth root tissue engineering. Nature Publishing Group 2017-04-12 /pmc/articles/PMC5388852/ /pubmed/28401887 http://dx.doi.org/10.1038/srep44635 Text en Copyright © 2017, The Author(s) http://creativecommons.org/licenses/by/4.0/ This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/
spellingShingle Article
Luo, Shiwei
Pei, Fei
Zhang, Wen
Guo, Weihua
Li, Rui
He, Wei
Tian, Weidong
Bone marrow mesenchymal stem cells combine with Treated dentin matrix to build biological root
title Bone marrow mesenchymal stem cells combine with Treated dentin matrix to build biological root
title_full Bone marrow mesenchymal stem cells combine with Treated dentin matrix to build biological root
title_fullStr Bone marrow mesenchymal stem cells combine with Treated dentin matrix to build biological root
title_full_unstemmed Bone marrow mesenchymal stem cells combine with Treated dentin matrix to build biological root
title_short Bone marrow mesenchymal stem cells combine with Treated dentin matrix to build biological root
title_sort bone marrow mesenchymal stem cells combine with treated dentin matrix to build biological root
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5388852/
https://www.ncbi.nlm.nih.gov/pubmed/28401887
http://dx.doi.org/10.1038/srep44635
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