Advances in pancreatic islet monolayer culture on glass surfaces enable super-resolution microscopy and insights into beta cell ciliogenesis and proliferation

A robust and reproducible method for culturing monolayers of adherent and well-spread primary islet cells on glass coverslips is required for detailed imaging studies by super-resolution and live-cell microscopy. Guided by an observation that dispersed islet cells spread and adhere well on glass sur...

Descripción completa

Detalles Bibliográficos
Autores principales: Phelps, Edward A., Cianciaruso, Chiara, Santo-Domingo, Jaime, Pasquier, Miriella, Galliverti, Gabriele, Piemonti, Lorenzo, Berishvili, Ekaterine, Burri, Olivier, Wiederkehr, Andreas, Hubbell, Jeffrey A., Baekkeskov, Steinunn
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group 2017
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5388888/
https://www.ncbi.nlm.nih.gov/pubmed/28401888
http://dx.doi.org/10.1038/srep45961

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5388888/
https://www.ncbi.nlm.nih.gov/pubmed/28401888
http://dx.doi.org/10.1038/srep45961

Ejemplares similares