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Advances in pancreatic islet monolayer culture on glass surfaces enable super-resolution microscopy and insights into beta cell ciliogenesis and proliferation
A robust and reproducible method for culturing monolayers of adherent and well-spread primary islet cells on glass coverslips is required for detailed imaging studies by super-resolution and live-cell microscopy. Guided by an observation that dispersed islet cells spread and adhere well on glass sur...
Autores principales: | Phelps, Edward A., Cianciaruso, Chiara, Santo-Domingo, Jaime, Pasquier, Miriella, Galliverti, Gabriele, Piemonti, Lorenzo, Berishvili, Ekaterine, Burri, Olivier, Wiederkehr, Andreas, Hubbell, Jeffrey A., Baekkeskov, Steinunn |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Nature Publishing Group
2017
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5388888/ https://www.ncbi.nlm.nih.gov/pubmed/28401888 http://dx.doi.org/10.1038/srep45961 |
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