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Quantitative photoacoustic integrating sphere (QPAIS) platform for absorption coefficient and Grüneisen parameter measurements: Demonstration with human blood

Quantitative photoacoustic imaging in biomedicine relies on accurate measurements of relevant material properties of target absorbers. Here, we present a method for simultaneous measurements of the absorption coefficient and Grüneisen parameter of small volume of liquid scattering and absorbing medi...

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Autores principales: Villanueva-Palero, Yolanda, Hondebrink, Erwin, Petersen, Wilma, Steenbergen, Wiendelt
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5388938/
https://www.ncbi.nlm.nih.gov/pubmed/28417067
http://dx.doi.org/10.1016/j.pacs.2017.03.004
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author Villanueva-Palero, Yolanda
Hondebrink, Erwin
Petersen, Wilma
Steenbergen, Wiendelt
author_facet Villanueva-Palero, Yolanda
Hondebrink, Erwin
Petersen, Wilma
Steenbergen, Wiendelt
author_sort Villanueva-Palero, Yolanda
collection PubMed
description Quantitative photoacoustic imaging in biomedicine relies on accurate measurements of relevant material properties of target absorbers. Here, we present a method for simultaneous measurements of the absorption coefficient and Grüneisen parameter of small volume of liquid scattering and absorbing media using a coupled-integrating sphere system which we refer to as quantitative photoacoustic integrating sphere (QPAIS) platform. The derived equations do not require absolute magnitudes of optical energy and pressure values, only calibration of the setup using aqueous ink dilutions is necessary. As a demonstration, measurements with blood samples from various human donors are done at room and body temperatures using an incubator. Measured absorption coefficient values are consistent with known oxygen saturation dependence of blood absorption at 750 nm, whereas measured Grüneisen parameter values indicate variability among five different donors. An increasing Grüneisen parameter value with both hematocrit and temperature is observed. These observations are consistent with those reported in literature.
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spelling pubmed-53889382017-04-17 Quantitative photoacoustic integrating sphere (QPAIS) platform for absorption coefficient and Grüneisen parameter measurements: Demonstration with human blood Villanueva-Palero, Yolanda Hondebrink, Erwin Petersen, Wilma Steenbergen, Wiendelt Photoacoustics Research Article Quantitative photoacoustic imaging in biomedicine relies on accurate measurements of relevant material properties of target absorbers. Here, we present a method for simultaneous measurements of the absorption coefficient and Grüneisen parameter of small volume of liquid scattering and absorbing media using a coupled-integrating sphere system which we refer to as quantitative photoacoustic integrating sphere (QPAIS) platform. The derived equations do not require absolute magnitudes of optical energy and pressure values, only calibration of the setup using aqueous ink dilutions is necessary. As a demonstration, measurements with blood samples from various human donors are done at room and body temperatures using an incubator. Measured absorption coefficient values are consistent with known oxygen saturation dependence of blood absorption at 750 nm, whereas measured Grüneisen parameter values indicate variability among five different donors. An increasing Grüneisen parameter value with both hematocrit and temperature is observed. These observations are consistent with those reported in literature. Elsevier 2017-03-24 /pmc/articles/PMC5388938/ /pubmed/28417067 http://dx.doi.org/10.1016/j.pacs.2017.03.004 Text en © 2017 The Authors http://creativecommons.org/licenses/by-nc-nd/4.0/ This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
spellingShingle Research Article
Villanueva-Palero, Yolanda
Hondebrink, Erwin
Petersen, Wilma
Steenbergen, Wiendelt
Quantitative photoacoustic integrating sphere (QPAIS) platform for absorption coefficient and Grüneisen parameter measurements: Demonstration with human blood
title Quantitative photoacoustic integrating sphere (QPAIS) platform for absorption coefficient and Grüneisen parameter measurements: Demonstration with human blood
title_full Quantitative photoacoustic integrating sphere (QPAIS) platform for absorption coefficient and Grüneisen parameter measurements: Demonstration with human blood
title_fullStr Quantitative photoacoustic integrating sphere (QPAIS) platform for absorption coefficient and Grüneisen parameter measurements: Demonstration with human blood
title_full_unstemmed Quantitative photoacoustic integrating sphere (QPAIS) platform for absorption coefficient and Grüneisen parameter measurements: Demonstration with human blood
title_short Quantitative photoacoustic integrating sphere (QPAIS) platform for absorption coefficient and Grüneisen parameter measurements: Demonstration with human blood
title_sort quantitative photoacoustic integrating sphere (qpais) platform for absorption coefficient and grüneisen parameter measurements: demonstration with human blood
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5388938/
https://www.ncbi.nlm.nih.gov/pubmed/28417067
http://dx.doi.org/10.1016/j.pacs.2017.03.004
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