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Quantitative photoacoustic integrating sphere (QPAIS) platform for absorption coefficient and Grüneisen parameter measurements: Demonstration with human blood
Quantitative photoacoustic imaging in biomedicine relies on accurate measurements of relevant material properties of target absorbers. Here, we present a method for simultaneous measurements of the absorption coefficient and Grüneisen parameter of small volume of liquid scattering and absorbing medi...
Autores principales: | , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Elsevier
2017
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5388938/ https://www.ncbi.nlm.nih.gov/pubmed/28417067 http://dx.doi.org/10.1016/j.pacs.2017.03.004 |
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author | Villanueva-Palero, Yolanda Hondebrink, Erwin Petersen, Wilma Steenbergen, Wiendelt |
author_facet | Villanueva-Palero, Yolanda Hondebrink, Erwin Petersen, Wilma Steenbergen, Wiendelt |
author_sort | Villanueva-Palero, Yolanda |
collection | PubMed |
description | Quantitative photoacoustic imaging in biomedicine relies on accurate measurements of relevant material properties of target absorbers. Here, we present a method for simultaneous measurements of the absorption coefficient and Grüneisen parameter of small volume of liquid scattering and absorbing media using a coupled-integrating sphere system which we refer to as quantitative photoacoustic integrating sphere (QPAIS) platform. The derived equations do not require absolute magnitudes of optical energy and pressure values, only calibration of the setup using aqueous ink dilutions is necessary. As a demonstration, measurements with blood samples from various human donors are done at room and body temperatures using an incubator. Measured absorption coefficient values are consistent with known oxygen saturation dependence of blood absorption at 750 nm, whereas measured Grüneisen parameter values indicate variability among five different donors. An increasing Grüneisen parameter value with both hematocrit and temperature is observed. These observations are consistent with those reported in literature. |
format | Online Article Text |
id | pubmed-5388938 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2017 |
publisher | Elsevier |
record_format | MEDLINE/PubMed |
spelling | pubmed-53889382017-04-17 Quantitative photoacoustic integrating sphere (QPAIS) platform for absorption coefficient and Grüneisen parameter measurements: Demonstration with human blood Villanueva-Palero, Yolanda Hondebrink, Erwin Petersen, Wilma Steenbergen, Wiendelt Photoacoustics Research Article Quantitative photoacoustic imaging in biomedicine relies on accurate measurements of relevant material properties of target absorbers. Here, we present a method for simultaneous measurements of the absorption coefficient and Grüneisen parameter of small volume of liquid scattering and absorbing media using a coupled-integrating sphere system which we refer to as quantitative photoacoustic integrating sphere (QPAIS) platform. The derived equations do not require absolute magnitudes of optical energy and pressure values, only calibration of the setup using aqueous ink dilutions is necessary. As a demonstration, measurements with blood samples from various human donors are done at room and body temperatures using an incubator. Measured absorption coefficient values are consistent with known oxygen saturation dependence of blood absorption at 750 nm, whereas measured Grüneisen parameter values indicate variability among five different donors. An increasing Grüneisen parameter value with both hematocrit and temperature is observed. These observations are consistent with those reported in literature. Elsevier 2017-03-24 /pmc/articles/PMC5388938/ /pubmed/28417067 http://dx.doi.org/10.1016/j.pacs.2017.03.004 Text en © 2017 The Authors http://creativecommons.org/licenses/by-nc-nd/4.0/ This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/). |
spellingShingle | Research Article Villanueva-Palero, Yolanda Hondebrink, Erwin Petersen, Wilma Steenbergen, Wiendelt Quantitative photoacoustic integrating sphere (QPAIS) platform for absorption coefficient and Grüneisen parameter measurements: Demonstration with human blood |
title | Quantitative photoacoustic integrating sphere (QPAIS) platform for absorption coefficient and Grüneisen parameter measurements: Demonstration with human blood |
title_full | Quantitative photoacoustic integrating sphere (QPAIS) platform for absorption coefficient and Grüneisen parameter measurements: Demonstration with human blood |
title_fullStr | Quantitative photoacoustic integrating sphere (QPAIS) platform for absorption coefficient and Grüneisen parameter measurements: Demonstration with human blood |
title_full_unstemmed | Quantitative photoacoustic integrating sphere (QPAIS) platform for absorption coefficient and Grüneisen parameter measurements: Demonstration with human blood |
title_short | Quantitative photoacoustic integrating sphere (QPAIS) platform for absorption coefficient and Grüneisen parameter measurements: Demonstration with human blood |
title_sort | quantitative photoacoustic integrating sphere (qpais) platform for absorption coefficient and grüneisen parameter measurements: demonstration with human blood |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5388938/ https://www.ncbi.nlm.nih.gov/pubmed/28417067 http://dx.doi.org/10.1016/j.pacs.2017.03.004 |
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