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Characterization of c-Maf(+)Foxp3(−) Regulatory T Cells Induced by Repeated Stimulation of Antigen-Presenting B Cells
The role of B cells in the development of CD4(+) regulatory T cells has been emphasized recently. Our previous studies have demonstrated that the antigen-presenting splenic B cells converted naïve CD4(+)CD25(−) T cells into CD4(+)CD25(+)Foxp3(−) T cells without additional cytokines or chemicals with...
Autores principales: | , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Nature Publishing Group
2017
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5389357/ https://www.ncbi.nlm.nih.gov/pubmed/28402334 http://dx.doi.org/10.1038/srep46348 |
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author | Chien, Chien-Hui Yu, Hui-Chieh Chen, Szu-Ying Chiang, Bor-Luen |
author_facet | Chien, Chien-Hui Yu, Hui-Chieh Chen, Szu-Ying Chiang, Bor-Luen |
author_sort | Chien, Chien-Hui |
collection | PubMed |
description | The role of B cells in the development of CD4(+) regulatory T cells has been emphasized recently. Our previous studies have demonstrated that the antigen-presenting splenic B cells converted naïve CD4(+)CD25(−) T cells into CD4(+)CD25(+)Foxp3(−) T cells without additional cytokines or chemicals with regulatory activity and that referred to as Treg-of-B cells. The present study further showed that Treg-of-B cells increased the IL-10-producing population, and the expression of c-Maf, inducible T-cell co-stimulator (ICOS) as well as cytotoxic T-lymphocyte-associated protein 4 (CTLA4) after repeated stimulation of B cells in a cell-cell contact-dependent manner. Long-term cultured Treg-of-B cells exerted IL-10 and CTLA4-mediated antigen-specific suppressive activity; moreover, the single antigen-specific Treg-of-B cells inhibited in a non-antigen-specific fashion. In conclusion, these results suggest that repeated stimulation of B cells induced IL-10-producing CD4(+)Foxp3(−) regulatory T cells in a contact-dependent manner and these Treg-of-B cells possess IL-10 and CTLA4-dependent suppressive function. |
format | Online Article Text |
id | pubmed-5389357 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2017 |
publisher | Nature Publishing Group |
record_format | MEDLINE/PubMed |
spelling | pubmed-53893572017-04-14 Characterization of c-Maf(+)Foxp3(−) Regulatory T Cells Induced by Repeated Stimulation of Antigen-Presenting B Cells Chien, Chien-Hui Yu, Hui-Chieh Chen, Szu-Ying Chiang, Bor-Luen Sci Rep Article The role of B cells in the development of CD4(+) regulatory T cells has been emphasized recently. Our previous studies have demonstrated that the antigen-presenting splenic B cells converted naïve CD4(+)CD25(−) T cells into CD4(+)CD25(+)Foxp3(−) T cells without additional cytokines or chemicals with regulatory activity and that referred to as Treg-of-B cells. The present study further showed that Treg-of-B cells increased the IL-10-producing population, and the expression of c-Maf, inducible T-cell co-stimulator (ICOS) as well as cytotoxic T-lymphocyte-associated protein 4 (CTLA4) after repeated stimulation of B cells in a cell-cell contact-dependent manner. Long-term cultured Treg-of-B cells exerted IL-10 and CTLA4-mediated antigen-specific suppressive activity; moreover, the single antigen-specific Treg-of-B cells inhibited in a non-antigen-specific fashion. In conclusion, these results suggest that repeated stimulation of B cells induced IL-10-producing CD4(+)Foxp3(−) regulatory T cells in a contact-dependent manner and these Treg-of-B cells possess IL-10 and CTLA4-dependent suppressive function. Nature Publishing Group 2017-04-12 /pmc/articles/PMC5389357/ /pubmed/28402334 http://dx.doi.org/10.1038/srep46348 Text en Copyright © 2017, The Author(s) http://creativecommons.org/licenses/by/4.0/ This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/ |
spellingShingle | Article Chien, Chien-Hui Yu, Hui-Chieh Chen, Szu-Ying Chiang, Bor-Luen Characterization of c-Maf(+)Foxp3(−) Regulatory T Cells Induced by Repeated Stimulation of Antigen-Presenting B Cells |
title | Characterization of c-Maf(+)Foxp3(−) Regulatory T Cells Induced by Repeated Stimulation of Antigen-Presenting B Cells |
title_full | Characterization of c-Maf(+)Foxp3(−) Regulatory T Cells Induced by Repeated Stimulation of Antigen-Presenting B Cells |
title_fullStr | Characterization of c-Maf(+)Foxp3(−) Regulatory T Cells Induced by Repeated Stimulation of Antigen-Presenting B Cells |
title_full_unstemmed | Characterization of c-Maf(+)Foxp3(−) Regulatory T Cells Induced by Repeated Stimulation of Antigen-Presenting B Cells |
title_short | Characterization of c-Maf(+)Foxp3(−) Regulatory T Cells Induced by Repeated Stimulation of Antigen-Presenting B Cells |
title_sort | characterization of c-maf(+)foxp3(−) regulatory t cells induced by repeated stimulation of antigen-presenting b cells |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5389357/ https://www.ncbi.nlm.nih.gov/pubmed/28402334 http://dx.doi.org/10.1038/srep46348 |
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