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Characterization of c-Maf(+)Foxp3(−) Regulatory T Cells Induced by Repeated Stimulation of Antigen-Presenting B Cells

The role of B cells in the development of CD4(+) regulatory T cells has been emphasized recently. Our previous studies have demonstrated that the antigen-presenting splenic B cells converted naïve CD4(+)CD25(−) T cells into CD4(+)CD25(+)Foxp3(−) T cells without additional cytokines or chemicals with...

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Autores principales: Chien, Chien-Hui, Yu, Hui-Chieh, Chen, Szu-Ying, Chiang, Bor-Luen
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5389357/
https://www.ncbi.nlm.nih.gov/pubmed/28402334
http://dx.doi.org/10.1038/srep46348
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author Chien, Chien-Hui
Yu, Hui-Chieh
Chen, Szu-Ying
Chiang, Bor-Luen
author_facet Chien, Chien-Hui
Yu, Hui-Chieh
Chen, Szu-Ying
Chiang, Bor-Luen
author_sort Chien, Chien-Hui
collection PubMed
description The role of B cells in the development of CD4(+) regulatory T cells has been emphasized recently. Our previous studies have demonstrated that the antigen-presenting splenic B cells converted naïve CD4(+)CD25(−) T cells into CD4(+)CD25(+)Foxp3(−) T cells without additional cytokines or chemicals with regulatory activity and that referred to as Treg-of-B cells. The present study further showed that Treg-of-B cells increased the IL-10-producing population, and the expression of c-Maf, inducible T-cell co-stimulator (ICOS) as well as cytotoxic T-lymphocyte-associated protein 4 (CTLA4) after repeated stimulation of B cells in a cell-cell contact-dependent manner. Long-term cultured Treg-of-B cells exerted IL-10 and CTLA4-mediated antigen-specific suppressive activity; moreover, the single antigen-specific Treg-of-B cells inhibited in a non-antigen-specific fashion. In conclusion, these results suggest that repeated stimulation of B cells induced IL-10-producing CD4(+)Foxp3(−) regulatory T cells in a contact-dependent manner and these Treg-of-B cells possess IL-10 and CTLA4-dependent suppressive function.
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spelling pubmed-53893572017-04-14 Characterization of c-Maf(+)Foxp3(−) Regulatory T Cells Induced by Repeated Stimulation of Antigen-Presenting B Cells Chien, Chien-Hui Yu, Hui-Chieh Chen, Szu-Ying Chiang, Bor-Luen Sci Rep Article The role of B cells in the development of CD4(+) regulatory T cells has been emphasized recently. Our previous studies have demonstrated that the antigen-presenting splenic B cells converted naïve CD4(+)CD25(−) T cells into CD4(+)CD25(+)Foxp3(−) T cells without additional cytokines or chemicals with regulatory activity and that referred to as Treg-of-B cells. The present study further showed that Treg-of-B cells increased the IL-10-producing population, and the expression of c-Maf, inducible T-cell co-stimulator (ICOS) as well as cytotoxic T-lymphocyte-associated protein 4 (CTLA4) after repeated stimulation of B cells in a cell-cell contact-dependent manner. Long-term cultured Treg-of-B cells exerted IL-10 and CTLA4-mediated antigen-specific suppressive activity; moreover, the single antigen-specific Treg-of-B cells inhibited in a non-antigen-specific fashion. In conclusion, these results suggest that repeated stimulation of B cells induced IL-10-producing CD4(+)Foxp3(−) regulatory T cells in a contact-dependent manner and these Treg-of-B cells possess IL-10 and CTLA4-dependent suppressive function. Nature Publishing Group 2017-04-12 /pmc/articles/PMC5389357/ /pubmed/28402334 http://dx.doi.org/10.1038/srep46348 Text en Copyright © 2017, The Author(s) http://creativecommons.org/licenses/by/4.0/ This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/
spellingShingle Article
Chien, Chien-Hui
Yu, Hui-Chieh
Chen, Szu-Ying
Chiang, Bor-Luen
Characterization of c-Maf(+)Foxp3(−) Regulatory T Cells Induced by Repeated Stimulation of Antigen-Presenting B Cells
title Characterization of c-Maf(+)Foxp3(−) Regulatory T Cells Induced by Repeated Stimulation of Antigen-Presenting B Cells
title_full Characterization of c-Maf(+)Foxp3(−) Regulatory T Cells Induced by Repeated Stimulation of Antigen-Presenting B Cells
title_fullStr Characterization of c-Maf(+)Foxp3(−) Regulatory T Cells Induced by Repeated Stimulation of Antigen-Presenting B Cells
title_full_unstemmed Characterization of c-Maf(+)Foxp3(−) Regulatory T Cells Induced by Repeated Stimulation of Antigen-Presenting B Cells
title_short Characterization of c-Maf(+)Foxp3(−) Regulatory T Cells Induced by Repeated Stimulation of Antigen-Presenting B Cells
title_sort characterization of c-maf(+)foxp3(−) regulatory t cells induced by repeated stimulation of antigen-presenting b cells
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5389357/
https://www.ncbi.nlm.nih.gov/pubmed/28402334
http://dx.doi.org/10.1038/srep46348
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