Cargando…

Spacer-length DNA intermediates are associated with Cas1 in cells undergoing primed CRISPR adaptation

During primed CRISPR adaptation spacers are preferentially selected from DNA recognized by CRISPR interference machinery, which in the case of Type I CRISPR–Cas systems consists of CRISPR RNA (crRNA) bound effector Cascade complex that locates complementary targets, and Cas3 executor nuclease/helica...

Descripción completa

Detalles Bibliográficos
Autores principales:	Musharova, Olga, Klimuk, Evgeny, Datsenko, Kirill A., Metlitskaya, Anastasia, Logacheva, Maria, Semenova, Ekaterina, Severinov, Konstantin, Savitskaya, Ekaterina
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	Oxford University Press 2017
Materias:	Molecular Biology
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5389516/ https://www.ncbi.nlm.nih.gov/pubmed/28204574 http://dx.doi.org/10.1093/nar/gkx097

Ejemplares similares

Detection of spacer precursors formed in vivo during primed CRISPR adaptation
por: Shiriaeva, Anna A., et al.
Publicado: (2019)

Pervasive generation of oppositely oriented spacers during CRISPR adaptation
por: Shmakov, Sergey, et al.
Publicado: (2014)

The action of Escherichia coli CRISPR–Cas system on lytic bacteriophages with different lifestyles and development strategies
por: Strotskaya, Alexandra, et al.
Publicado: (2017)

Prespacers formed during primed adaptation associate with the Cas1–Cas2 adaptation complex and the Cas3 interference nuclease–helicase
por: Musharova, Olga, et al.
Publicado: (2021)

Systematic analysis of Type I‐E Escherichia coli CRISPR‐Cas PAM sequences ability to promote interference and primed adaptation
por: Musharova, Olga, et al.
Publicado: (2019)

The Cas6e ribonuclease is not required for interference and adaptation by the E. coli type I-E CRISPR-Cas system
por: Semenova, Ekaterina, et al.
Publicado: (2015)

Genome Maintenance Proteins Modulate Autoimmunity Mediated Primed Adaptation by the Escherichia coli Type I-E CRISPR-Cas System
por: Kurilovich, Elena, et al.
Publicado: (2019)

Altered stoichiometry Escherichia coli Cascade complexes with shortened CRISPR RNA spacers are capable of interference and primed adaptation
por: Kuznedelov, Konstantin, et al.
Publicado: (2016)

Foreign DNA acquisition by the I-F CRISPR–Cas system requires all components of the interference machinery
por: Vorontsova, Daria, et al.
Publicado: (2015)

CRISPR interference and priming varies with individual spacer sequences
por: Xue, Chaoyou, et al.
Publicado: (2015)

Host nucleases generate prespacers for primed adaptation in the E. coli type I-E CRISPR-Cas system
por: Shiriaeva, Anna A., et al.
Publicado: (2022)

Spacer acquisition by Type III CRISPR–Cas system during bacteriophage infection of Thermus thermophilus
por: Artamonova, Daria, et al.
Publicado: (2020)

Function of the CRISPR-Cas System of the Human Pathogen Clostridium difficile
por: Boudry, Pierre, et al.
Publicado: (2015)

Type I-E CRISPR-Cas Systems Discriminate Target from Non-Target DNA through Base Pairing-Independent PAM Recognition
por: Westra, Edze R., et al.
Publicado: (2013)

The Influence of Copy-Number of Targeted Extrachromosomal Genetic Elements on the Outcome of CRISPR-Cas Defense
por: Severinov, Konstantin, et al.
Publicado: (2016)

Avoidance of Trinucleotide Corresponding to Consensus Protospacer Adjacent Motif Controls the Efficiency of Prespacer Selection during Primed Adaptation
por: Musharova, Olga, et al.
Publicado: (2018)

Erratum for Boudry et al., Function of the CRISPR-Cas System of the Human Pathogen Clostridium difficile
por: Boudry, Pierre, et al.
Publicado: (2015)

Defining the seed sequence of the Cas12b CRISPR-Cas effector complex
por: Jain, Ishita, et al.
Publicado: (2018)

Position of Deltaproteobacteria Cas12e nuclease cleavage sites depends on spacer length of guide RNA
por: Selkova, Polina, et al.
Publicado: (2020)

Kinetics of the CRISPR-Cas9 effector complex assembly and the role of 3′-terminal segment of guide RNA
por: Mekler, Vladimir, et al.
Publicado: (2016)

CRISPR-Cas Controls Cryptic Prophages
por: Song, Sooyeon, et al.
Publicado: (2022)

CRISPR RNA binding and DNA target recognition by purified Cascade complexes from Escherichia coli
por: Beloglazova, Natalia, et al.
Publicado: (2015)

Optimal number of spacers in CRISPR arrays
por: Martynov, Alexander, et al.
Publicado: (2017)

Primed CRISPR adaptation in Escherichia coli cells does not depend on conformational changes in the Cascade effector complex detected in Vitro
por: Krivoy, Andrey, et al.
Publicado: (2018)

Natural diversity of CRISPR spacers of Thermus: evidence of local spacer acquisition and global spacer exchange
por: Lopatina, Anna, et al.
Publicado: (2019)

Protospacer-Adjacent Motif Specificity during Clostridioides difficile Type I-B CRISPR-Cas Interference and Adaptation
por: Maikova, Anna, et al.
Publicado: (2021)

Mapping CRISPR spaceromes reveals vast host-specific viromes of prokaryotes
por: Shmakov, Sergey A., et al.
Publicado: (2020)

Detection and characterization of spacer integration intermediates in type I-E CRISPR–Cas system
por: Arslan, Zihni, et al.
Publicado: (2014)

Uncertainty-aware and interpretable evaluation of Cas9–gRNA and Cas12a–gRNA specificity for fully matched and partially mismatched targets with Deep Kernel Learning
por: Kirillov, Bogdan, et al.
Publicado: (2021)

The CRISPR Spacer Space Is Dominated by Sequences from Species-Specific Mobilomes
por: Shmakov, Sergey A., et al.
Publicado: (2017)

Priming in the Type I-F CRISPR-Cas system triggers strand-independent spacer acquisition, bi-directionally from the primed protospacer
por: Richter, Corinna, et al.
Publicado: (2014)

Controller protein of restriction–modification system Kpn2I affects transcription of its gene by acting as a transcription elongation roadblock
por: Klimuk, Evgeny, et al.
Publicado: (2018)

Interference-driven spacer acquisition is dominant over naive and primed adaptation in a native CRISPR–Cas system
por: Staals, Raymond H. J., et al.
Publicado: (2016)

Persistence of plasmids targeted by CRISPR interference in bacterial populations
por: Mamontov, Viktor, et al.
Publicado: (2022)

Regulation of Gene Expression of phiEco32-like Bacteriophage 7-11
por: Lavysh, Daria, et al.
Publicado: (2022)

DNA targeting by Clostridium cellulolyticum CRISPR–Cas9 Type II-C system
por: Fedorova, Iana, et al.
Publicado: (2020)

CRISPR-Cas Systems and the Paradox of Self-Targeting Spacers
por: Wimmer, Franziska, et al.
Publicado: (2020)

Priming in a permissive type I-C CRISPR–Cas system reveals distinct dynamics of spacer acquisition and loss
por: Rao, Chitong, et al.
Publicado: (2017)

CRISPR DNA elements controlling site-specific spacer integration and proper repeat length by a Type II CRISPR–Cas system
por: Kim, Jenny G, et al.
Publicado: (2019)

New Insights Into Functions and Possible Applications of Clostridium difficile CRISPR-Cas System
por: Maikova, Anna, et al.
Publicado: (2018)

Cannot write session to /tmp/vufind_sessions/sess_4st3v7s2mp47kueoh63p493lpi