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Spacer-length DNA intermediates are associated with Cas1 in cells undergoing primed CRISPR adaptation

During primed CRISPR adaptation spacers are preferentially selected from DNA recognized by CRISPR interference machinery, which in the case of Type I CRISPR–Cas systems consists of CRISPR RNA (crRNA) bound effector Cascade complex that locates complementary targets, and Cas3 executor nuclease/helica...

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Detalles Bibliográficos
Autores principales:	Musharova, Olga, Klimuk, Evgeny, Datsenko, Kirill A., Metlitskaya, Anastasia, Logacheva, Maria, Semenova, Ekaterina, Severinov, Konstantin, Savitskaya, Ekaterina
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	Oxford University Press 2017
Materias:	Molecular Biology
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5389516/ https://www.ncbi.nlm.nih.gov/pubmed/28204574 http://dx.doi.org/10.1093/nar/gkx097

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