Cargando…

CRISPR-Cas type I-A Cascade complex couples viral infection surveillance to host transcriptional regulation in the dependence of Csa3b

CRISPR-Cas (clustered regularly interspaced short palindromic repeats and the associated genes) constitute adaptive immune systems in bacteria and archaea and they provide sequence specific immunity against foreign nucleic acids. CRISPR-Cas systems are activated by viral infection. However, little i...

Descripción completa

Detalles Bibliográficos
Autores principales: He, Fei, Vestergaard, Gisle, Peng, Wenfang, She, Qunxin, Peng, Xu
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Oxford University Press 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5389559/
https://www.ncbi.nlm.nih.gov/pubmed/27980065
http://dx.doi.org/10.1093/nar/gkw1265
_version_ 1782521291985649664
author He, Fei
Vestergaard, Gisle
Peng, Wenfang
She, Qunxin
Peng, Xu
author_facet He, Fei
Vestergaard, Gisle
Peng, Wenfang
She, Qunxin
Peng, Xu
author_sort He, Fei
collection PubMed
description CRISPR-Cas (clustered regularly interspaced short palindromic repeats and the associated genes) constitute adaptive immune systems in bacteria and archaea and they provide sequence specific immunity against foreign nucleic acids. CRISPR-Cas systems are activated by viral infection. However, little is known about how CRISPR-Cas systems are activated in response to viral infection or how their expression is controlled in the absence of viral infection. Here, we demonstrate that both the transcriptional regulator Csa3b, and the type I-A interference complex Cascade, are required to transcriptionally repress the interference gene cassette in the archaeon Sulfolobus. Csa3b binds to two palindromic repeat sites in the promoter region of the cassette and facilitates binding of the Cascade to the promoter region. Upon viral infection, loading of Cascade complexes onto crRNA-matching protospacers leads to relief of the transcriptional repression. Our data demonstrate a mechanism coupling CRISPR-Cas surveillance of protospacers to transcriptional regulation of the interference gene cassette thereby allowing a fast response to viral infection.
format Online
Article
Text
id pubmed-5389559
institution National Center for Biotechnology Information
language English
publishDate 2017
publisher Oxford University Press
record_format MEDLINE/PubMed
spelling pubmed-53895592017-04-24 CRISPR-Cas type I-A Cascade complex couples viral infection surveillance to host transcriptional regulation in the dependence of Csa3b He, Fei Vestergaard, Gisle Peng, Wenfang She, Qunxin Peng, Xu Nucleic Acids Res Molecular Biology CRISPR-Cas (clustered regularly interspaced short palindromic repeats and the associated genes) constitute adaptive immune systems in bacteria and archaea and they provide sequence specific immunity against foreign nucleic acids. CRISPR-Cas systems are activated by viral infection. However, little is known about how CRISPR-Cas systems are activated in response to viral infection or how their expression is controlled in the absence of viral infection. Here, we demonstrate that both the transcriptional regulator Csa3b, and the type I-A interference complex Cascade, are required to transcriptionally repress the interference gene cassette in the archaeon Sulfolobus. Csa3b binds to two palindromic repeat sites in the promoter region of the cassette and facilitates binding of the Cascade to the promoter region. Upon viral infection, loading of Cascade complexes onto crRNA-matching protospacers leads to relief of the transcriptional repression. Our data demonstrate a mechanism coupling CRISPR-Cas surveillance of protospacers to transcriptional regulation of the interference gene cassette thereby allowing a fast response to viral infection. Oxford University Press 2017-02-28 2016-12-15 /pmc/articles/PMC5389559/ /pubmed/27980065 http://dx.doi.org/10.1093/nar/gkw1265 Text en © The Author(s) 2016. Published by Oxford University Press on behalf of Nucleic Acids Research. http://creativecommons.org/licenses/by-nc/4.0/ This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by-nc/4.0/), which permits non-commercial re-use, distribution, and reproduction in any medium, provided the original work is properly cited. For commercial re-use, please contact journals.permissions@oup.com
spellingShingle Molecular Biology
He, Fei
Vestergaard, Gisle
Peng, Wenfang
She, Qunxin
Peng, Xu
CRISPR-Cas type I-A Cascade complex couples viral infection surveillance to host transcriptional regulation in the dependence of Csa3b
title CRISPR-Cas type I-A Cascade complex couples viral infection surveillance to host transcriptional regulation in the dependence of Csa3b
title_full CRISPR-Cas type I-A Cascade complex couples viral infection surveillance to host transcriptional regulation in the dependence of Csa3b
title_fullStr CRISPR-Cas type I-A Cascade complex couples viral infection surveillance to host transcriptional regulation in the dependence of Csa3b
title_full_unstemmed CRISPR-Cas type I-A Cascade complex couples viral infection surveillance to host transcriptional regulation in the dependence of Csa3b
title_short CRISPR-Cas type I-A Cascade complex couples viral infection surveillance to host transcriptional regulation in the dependence of Csa3b
title_sort crispr-cas type i-a cascade complex couples viral infection surveillance to host transcriptional regulation in the dependence of csa3b
topic Molecular Biology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5389559/
https://www.ncbi.nlm.nih.gov/pubmed/27980065
http://dx.doi.org/10.1093/nar/gkw1265
work_keys_str_mv AT hefei crisprcastypeiacascadecomplexcouplesviralinfectionsurveillancetohosttranscriptionalregulationinthedependenceofcsa3b
AT vestergaardgisle crisprcastypeiacascadecomplexcouplesviralinfectionsurveillancetohosttranscriptionalregulationinthedependenceofcsa3b
AT pengwenfang crisprcastypeiacascadecomplexcouplesviralinfectionsurveillancetohosttranscriptionalregulationinthedependenceofcsa3b
AT shequnxin crisprcastypeiacascadecomplexcouplesviralinfectionsurveillancetohosttranscriptionalregulationinthedependenceofcsa3b
AT pengxu crisprcastypeiacascadecomplexcouplesviralinfectionsurveillancetohosttranscriptionalregulationinthedependenceofcsa3b