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Antibacterial and antivirulence effect of 6-N-hydroxylaminopurine in Listeria monocytogenes
The emerging development of antibiotic resistant bacteria calls for novel types of antibacterial agents. In this work we examined the putative antibacterial effect of purine analogs in Listeria monocytogenes. We show that, among several tested purine analogs, only 6-N-hydroxylaminopurine (6-N-HAP) r...
Autores principales: | , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Oxford University Press
2017
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5389569/ https://www.ncbi.nlm.nih.gov/pubmed/28062853 http://dx.doi.org/10.1093/nar/gkw1308 |
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author | Krajewski, Stefanie Sandra Isoz, Isabelle Johansson, Jörgen |
author_facet | Krajewski, Stefanie Sandra Isoz, Isabelle Johansson, Jörgen |
author_sort | Krajewski, Stefanie Sandra |
collection | PubMed |
description | The emerging development of antibiotic resistant bacteria calls for novel types of antibacterial agents. In this work we examined the putative antibacterial effect of purine analogs in Listeria monocytogenes. We show that, among several tested purine analogs, only 6-N-hydroxylaminopurine (6-N-HAP) reduces the viability of the Gram-positive pathogen Listeria monocytogenes. As in Bacillus subtilis, 6-N-HAP terminates expression at guanine riboswitches in L. monocytogenes hence preventing expression of their downstream genes. However, we show that the bacteriocidal effect of the compound was unlinked to the terminated expression at the guanine riboswitches. When further examining the antimicrobial effect, we observed that 6-N-HAP acts as a potent mutagen in L. monocytogenes, by increasing the mutation rate and inducing the SOS-response. Also, addition of 6-N-HAP decreased virulence gene expression by reducing both the levels and activity of the virulence regulator PrfA. |
format | Online Article Text |
id | pubmed-5389569 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2017 |
publisher | Oxford University Press |
record_format | MEDLINE/PubMed |
spelling | pubmed-53895692017-04-24 Antibacterial and antivirulence effect of 6-N-hydroxylaminopurine in Listeria monocytogenes Krajewski, Stefanie Sandra Isoz, Isabelle Johansson, Jörgen Nucleic Acids Res Molecular Biology The emerging development of antibiotic resistant bacteria calls for novel types of antibacterial agents. In this work we examined the putative antibacterial effect of purine analogs in Listeria monocytogenes. We show that, among several tested purine analogs, only 6-N-hydroxylaminopurine (6-N-HAP) reduces the viability of the Gram-positive pathogen Listeria monocytogenes. As in Bacillus subtilis, 6-N-HAP terminates expression at guanine riboswitches in L. monocytogenes hence preventing expression of their downstream genes. However, we show that the bacteriocidal effect of the compound was unlinked to the terminated expression at the guanine riboswitches. When further examining the antimicrobial effect, we observed that 6-N-HAP acts as a potent mutagen in L. monocytogenes, by increasing the mutation rate and inducing the SOS-response. Also, addition of 6-N-HAP decreased virulence gene expression by reducing both the levels and activity of the virulence regulator PrfA. Oxford University Press 2017-02-28 2017-01-06 /pmc/articles/PMC5389569/ /pubmed/28062853 http://dx.doi.org/10.1093/nar/gkw1308 Text en © The Author(s) 2017. Published by Oxford University Press on behalf of Nucleic Acids Research. http://creativecommons.org/licenses/by-nc/4.0/ This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by-nc/4.0/), which permits non-commercial re-use, distribution, and reproduction in any medium, provided the original work is properly cited. For commercial re-use, please contact journals.permissions@oup.com |
spellingShingle | Molecular Biology Krajewski, Stefanie Sandra Isoz, Isabelle Johansson, Jörgen Antibacterial and antivirulence effect of 6-N-hydroxylaminopurine in Listeria monocytogenes |
title | Antibacterial and antivirulence effect of 6-N-hydroxylaminopurine in Listeria monocytogenes |
title_full | Antibacterial and antivirulence effect of 6-N-hydroxylaminopurine in Listeria monocytogenes |
title_fullStr | Antibacterial and antivirulence effect of 6-N-hydroxylaminopurine in Listeria monocytogenes |
title_full_unstemmed | Antibacterial and antivirulence effect of 6-N-hydroxylaminopurine in Listeria monocytogenes |
title_short | Antibacterial and antivirulence effect of 6-N-hydroxylaminopurine in Listeria monocytogenes |
title_sort | antibacterial and antivirulence effect of 6-n-hydroxylaminopurine in listeria monocytogenes |
topic | Molecular Biology |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5389569/ https://www.ncbi.nlm.nih.gov/pubmed/28062853 http://dx.doi.org/10.1093/nar/gkw1308 |
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