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Antibacterial and antivirulence effect of 6-N-hydroxylaminopurine in Listeria monocytogenes

The emerging development of antibiotic resistant bacteria calls for novel types of antibacterial agents. In this work we examined the putative antibacterial effect of purine analogs in Listeria monocytogenes. We show that, among several tested purine analogs, only 6-N-hydroxylaminopurine (6-N-HAP) r...

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Autores principales: Krajewski, Stefanie Sandra, Isoz, Isabelle, Johansson, Jörgen
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Oxford University Press 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5389569/
https://www.ncbi.nlm.nih.gov/pubmed/28062853
http://dx.doi.org/10.1093/nar/gkw1308
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author Krajewski, Stefanie Sandra
Isoz, Isabelle
Johansson, Jörgen
author_facet Krajewski, Stefanie Sandra
Isoz, Isabelle
Johansson, Jörgen
author_sort Krajewski, Stefanie Sandra
collection PubMed
description The emerging development of antibiotic resistant bacteria calls for novel types of antibacterial agents. In this work we examined the putative antibacterial effect of purine analogs in Listeria monocytogenes. We show that, among several tested purine analogs, only 6-N-hydroxylaminopurine (6-N-HAP) reduces the viability of the Gram-positive pathogen Listeria monocytogenes. As in Bacillus subtilis, 6-N-HAP terminates expression at guanine riboswitches in L. monocytogenes hence preventing expression of their downstream genes. However, we show that the bacteriocidal effect of the compound was unlinked to the terminated expression at the guanine riboswitches. When further examining the antimicrobial effect, we observed that 6-N-HAP acts as a potent mutagen in L. monocytogenes, by increasing the mutation rate and inducing the SOS-response. Also, addition of 6-N-HAP decreased virulence gene expression by reducing both the levels and activity of the virulence regulator PrfA.
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spelling pubmed-53895692017-04-24 Antibacterial and antivirulence effect of 6-N-hydroxylaminopurine in Listeria monocytogenes Krajewski, Stefanie Sandra Isoz, Isabelle Johansson, Jörgen Nucleic Acids Res Molecular Biology The emerging development of antibiotic resistant bacteria calls for novel types of antibacterial agents. In this work we examined the putative antibacterial effect of purine analogs in Listeria monocytogenes. We show that, among several tested purine analogs, only 6-N-hydroxylaminopurine (6-N-HAP) reduces the viability of the Gram-positive pathogen Listeria monocytogenes. As in Bacillus subtilis, 6-N-HAP terminates expression at guanine riboswitches in L. monocytogenes hence preventing expression of their downstream genes. However, we show that the bacteriocidal effect of the compound was unlinked to the terminated expression at the guanine riboswitches. When further examining the antimicrobial effect, we observed that 6-N-HAP acts as a potent mutagen in L. monocytogenes, by increasing the mutation rate and inducing the SOS-response. Also, addition of 6-N-HAP decreased virulence gene expression by reducing both the levels and activity of the virulence regulator PrfA. Oxford University Press 2017-02-28 2017-01-06 /pmc/articles/PMC5389569/ /pubmed/28062853 http://dx.doi.org/10.1093/nar/gkw1308 Text en © The Author(s) 2017. Published by Oxford University Press on behalf of Nucleic Acids Research. http://creativecommons.org/licenses/by-nc/4.0/ This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by-nc/4.0/), which permits non-commercial re-use, distribution, and reproduction in any medium, provided the original work is properly cited. For commercial re-use, please contact journals.permissions@oup.com
spellingShingle Molecular Biology
Krajewski, Stefanie Sandra
Isoz, Isabelle
Johansson, Jörgen
Antibacterial and antivirulence effect of 6-N-hydroxylaminopurine in Listeria monocytogenes
title Antibacterial and antivirulence effect of 6-N-hydroxylaminopurine in Listeria monocytogenes
title_full Antibacterial and antivirulence effect of 6-N-hydroxylaminopurine in Listeria monocytogenes
title_fullStr Antibacterial and antivirulence effect of 6-N-hydroxylaminopurine in Listeria monocytogenes
title_full_unstemmed Antibacterial and antivirulence effect of 6-N-hydroxylaminopurine in Listeria monocytogenes
title_short Antibacterial and antivirulence effect of 6-N-hydroxylaminopurine in Listeria monocytogenes
title_sort antibacterial and antivirulence effect of 6-n-hydroxylaminopurine in listeria monocytogenes
topic Molecular Biology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5389569/
https://www.ncbi.nlm.nih.gov/pubmed/28062853
http://dx.doi.org/10.1093/nar/gkw1308
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