Identification of 2-methylthio cyclic N(6)-threonylcarbamoyladenosine (ms(2)ct(6)A) as a novel RNA modification at position 37 of tRNAs

Transfer RNA modifications play pivotal roles in protein synthesis. N(6)-threonylcarbamoyladenosine (t(6)A) and its derivatives are modifications found at position 37, 3΄-adjacent to the anticodon, in tRNAs responsible for ANN codons. These modifications are universally conserved in all domains of l...

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Autores principales: Kang, Byeong-il, Miyauchi, Kenjyo, Matuszewski, Michal, D'Almeida, Gabriel Silveira, Rubio, Mary Anne T., Alfonzo, Juan D., Inoue, Kazuki, Sakaguchi, Yuriko, Suzuki, Takeo, Sochacka, Elzbieta, Suzuki, Tsutomu
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Oxford University Press 2017
Materias:
Gene regulation, Chromatin and Epigenetics
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5389704/
https://www.ncbi.nlm.nih.gov/pubmed/27913733
http://dx.doi.org/10.1093/nar/gkw1120
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author Kang, Byeong-il
Miyauchi, Kenjyo
Matuszewski, Michal
D'Almeida, Gabriel Silveira
Rubio, Mary Anne T.
Alfonzo, Juan D.
Inoue, Kazuki
Sakaguchi, Yuriko
Suzuki, Takeo
Sochacka, Elzbieta
Suzuki, Tsutomu
author_facet Kang, Byeong-il
Miyauchi, Kenjyo
Matuszewski, Michal
D'Almeida, Gabriel Silveira
Rubio, Mary Anne T.
Alfonzo, Juan D.
Inoue, Kazuki
Sakaguchi, Yuriko
Suzuki, Takeo
Sochacka, Elzbieta
Suzuki, Tsutomu
author_sort Kang, Byeong-il
collection PubMed
description Transfer RNA modifications play pivotal roles in protein synthesis. N(6)-threonylcarbamoyladenosine (t(6)A) and its derivatives are modifications found at position 37, 3΄-adjacent to the anticodon, in tRNAs responsible for ANN codons. These modifications are universally conserved in all domains of life. t(6)A and its derivatives have pleiotropic functions in protein synthesis including aminoacylation, decoding and translocation. We previously discovered a cyclic form of t(6)A (ct(6)A) as a chemically labile derivative of t(6)A in tRNAs from bacteria, fungi, plants and protists. Here, we report 2-methylthio cyclic t(6)A (ms(2)ct(6)A), a novel derivative of ct(6)A found in tRNAs from Bacillus subtilis, plants and Trypanosoma brucei. In B. subtilis and T. brucei, ms(2)ct(6)A disappeared and remained to be ms(2)t(6)A and ct(6)A by depletion of tcdA and mtaB homologs, respectively, demonstrating that TcdA and MtaB are responsible for biogenesis of ms(2)ct(6)A.
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spelling pubmed-53897042017-04-24 Identification of 2-methylthio cyclic N(6)-threonylcarbamoyladenosine (ms(2)ct(6)A) as a novel RNA modification at position 37 of tRNAs Kang, Byeong-il Miyauchi, Kenjyo Matuszewski, Michal D'Almeida, Gabriel Silveira Rubio, Mary Anne T. Alfonzo, Juan D. Inoue, Kazuki Sakaguchi, Yuriko Suzuki, Takeo Sochacka, Elzbieta Suzuki, Tsutomu Nucleic Acids Res Gene regulation, Chromatin and Epigenetics Transfer RNA modifications play pivotal roles in protein synthesis. N(6)-threonylcarbamoyladenosine (t(6)A) and its derivatives are modifications found at position 37, 3΄-adjacent to the anticodon, in tRNAs responsible for ANN codons. These modifications are universally conserved in all domains of life. t(6)A and its derivatives have pleiotropic functions in protein synthesis including aminoacylation, decoding and translocation. We previously discovered a cyclic form of t(6)A (ct(6)A) as a chemically labile derivative of t(6)A in tRNAs from bacteria, fungi, plants and protists. Here, we report 2-methylthio cyclic t(6)A (ms(2)ct(6)A), a novel derivative of ct(6)A found in tRNAs from Bacillus subtilis, plants and Trypanosoma brucei. In B. subtilis and T. brucei, ms(2)ct(6)A disappeared and remained to be ms(2)t(6)A and ct(6)A by depletion of tcdA and mtaB homologs, respectively, demonstrating that TcdA and MtaB are responsible for biogenesis of ms(2)ct(6)A. Oxford University Press 2017-02-28 2016-12-02 /pmc/articles/PMC5389704/ /pubmed/27913733 http://dx.doi.org/10.1093/nar/gkw1120 Text en © The Author(s) 2016. Published by Oxford University Press on behalf of Nucleic Acids Research. http://creativecommons.org/licenses/by-nc/4.0/ This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by-nc/4.0/), which permits non-commercial re-use, distribution, and reproduction in any medium, provided the original work is properly cited. For commercial re-use, please contact journals.permissions@oup.com
spellingShingle Gene regulation, Chromatin and Epigenetics
Kang, Byeong-il
Miyauchi, Kenjyo
Matuszewski, Michal
D'Almeida, Gabriel Silveira
Rubio, Mary Anne T.
Alfonzo, Juan D.
Inoue, Kazuki
Sakaguchi, Yuriko
Suzuki, Takeo
Sochacka, Elzbieta
Suzuki, Tsutomu
Identification of 2-methylthio cyclic N(6)-threonylcarbamoyladenosine (ms(2)ct(6)A) as a novel RNA modification at position 37 of tRNAs
title Identification of 2-methylthio cyclic N(6)-threonylcarbamoyladenosine (ms(2)ct(6)A) as a novel RNA modification at position 37 of tRNAs
title_full Identification of 2-methylthio cyclic N(6)-threonylcarbamoyladenosine (ms(2)ct(6)A) as a novel RNA modification at position 37 of tRNAs
title_fullStr Identification of 2-methylthio cyclic N(6)-threonylcarbamoyladenosine (ms(2)ct(6)A) as a novel RNA modification at position 37 of tRNAs
title_full_unstemmed Identification of 2-methylthio cyclic N(6)-threonylcarbamoyladenosine (ms(2)ct(6)A) as a novel RNA modification at position 37 of tRNAs
title_short Identification of 2-methylthio cyclic N(6)-threonylcarbamoyladenosine (ms(2)ct(6)A) as a novel RNA modification at position 37 of tRNAs
title_sort identification of 2-methylthio cyclic n(6)-threonylcarbamoyladenosine (ms(2)ct(6)a) as a novel rna modification at position 37 of trnas
topic Gene regulation, Chromatin and Epigenetics
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5389704/
https://www.ncbi.nlm.nih.gov/pubmed/27913733
http://dx.doi.org/10.1093/nar/gkw1120
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