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Integration-defective lentiviral vector mediates efficient gene editing through homology-directed repair in human embryonic stem cells

Human embryonic stem cells (hESCs) are used as platforms for disease study, drug screening and cell-based therapy. To facilitate these applications, it is frequently necessary to genetically manipulate the hESC genome. Gene editing with engineered nucleases enables site-specific genetic modification...

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Detalles Bibliográficos
Autores principales:	Wang, Yebo, Wang, Yingjia, Chang, Tammy, Huang, He, Yee, Jiing-Kuan
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	Oxford University Press 2017
Materias:	Methods Online
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5389720/ https://www.ncbi.nlm.nih.gov/pubmed/27899664 http://dx.doi.org/10.1093/nar/gkw1057

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