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Low prevalence of human enteropathogenic Yersinia spp. in brown rats (Rattus norvegicus) in Flanders

Brown rats (Rattus norvegicus) have been identified as potential carriers of Yersinia enterocolitica and Y. pseudotuberculosis, the etiological agents of yersiniosis, the third most reported bacterial zoonosis in Europe. Enteropathogenic Yersinia spp. are most often isolated from rats during yersini...

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Autores principales: Rouffaer, Lieze Oscar, Baert, Kristof, Van den Abeele, Anne-Marie, Cox, Ivo, Vanantwerpen, Gerty, De Zutter, Lieven, Strubbe, Diederik, Vranckx, Katleen, Lens, Luc, Haesebrouck, Freddy, Delmée, Michel, Pasmans, Frank, Martel, An
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5389835/
https://www.ncbi.nlm.nih.gov/pubmed/28403184
http://dx.doi.org/10.1371/journal.pone.0175648
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author Rouffaer, Lieze Oscar
Baert, Kristof
Van den Abeele, Anne-Marie
Cox, Ivo
Vanantwerpen, Gerty
De Zutter, Lieven
Strubbe, Diederik
Vranckx, Katleen
Lens, Luc
Haesebrouck, Freddy
Delmée, Michel
Pasmans, Frank
Martel, An
author_facet Rouffaer, Lieze Oscar
Baert, Kristof
Van den Abeele, Anne-Marie
Cox, Ivo
Vanantwerpen, Gerty
De Zutter, Lieven
Strubbe, Diederik
Vranckx, Katleen
Lens, Luc
Haesebrouck, Freddy
Delmée, Michel
Pasmans, Frank
Martel, An
author_sort Rouffaer, Lieze Oscar
collection PubMed
description Brown rats (Rattus norvegicus) have been identified as potential carriers of Yersinia enterocolitica and Y. pseudotuberculosis, the etiological agents of yersiniosis, the third most reported bacterial zoonosis in Europe. Enteropathogenic Yersinia spp. are most often isolated from rats during yersiniosis cases in animals and humans, and from rats inhabiting farms and slaughterhouses. Information is however lacking regarding the extent to which rats act as carriers of these Yersinia spp.. In 2013, 1088 brown rats across Flanders, Belgium, were tested for the presence of Yersinia species by isolation method. Identification was performed using MALDI-TOF MS, PCR on chromosomal- and plasmid-borne virulence genes, biotyping and serotyping. Yersinia spp. were isolated from 38.4% of the rats. Of these, 53.4% were designated Y. enterocolitica, 0.7% Y. pseudotuberculosis and 49.0% other Yersinia species. Two Y. enterocolitica possessing the virF-, ail- and ystA-gene were isolated. Additionally, the ystB-gene was identified in 94.1% of the other Y. enterocolitica isolates, suggestive for biotype 1A. Three of these latter isolates simultaneously possessed the ail-virulence gene. Significantly more Y. enterocolitica were isolated during winter and spring compared to summer. Based on our findings we can conclude that brown rats are frequent carriers for various Yersinia spp., including Y. pseudotuberculosis and (human pathogenic) Y. enterocolitica which are more often isolated during winter and spring.
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spelling pubmed-53898352017-05-03 Low prevalence of human enteropathogenic Yersinia spp. in brown rats (Rattus norvegicus) in Flanders Rouffaer, Lieze Oscar Baert, Kristof Van den Abeele, Anne-Marie Cox, Ivo Vanantwerpen, Gerty De Zutter, Lieven Strubbe, Diederik Vranckx, Katleen Lens, Luc Haesebrouck, Freddy Delmée, Michel Pasmans, Frank Martel, An PLoS One Research Article Brown rats (Rattus norvegicus) have been identified as potential carriers of Yersinia enterocolitica and Y. pseudotuberculosis, the etiological agents of yersiniosis, the third most reported bacterial zoonosis in Europe. Enteropathogenic Yersinia spp. are most often isolated from rats during yersiniosis cases in animals and humans, and from rats inhabiting farms and slaughterhouses. Information is however lacking regarding the extent to which rats act as carriers of these Yersinia spp.. In 2013, 1088 brown rats across Flanders, Belgium, were tested for the presence of Yersinia species by isolation method. Identification was performed using MALDI-TOF MS, PCR on chromosomal- and plasmid-borne virulence genes, biotyping and serotyping. Yersinia spp. were isolated from 38.4% of the rats. Of these, 53.4% were designated Y. enterocolitica, 0.7% Y. pseudotuberculosis and 49.0% other Yersinia species. Two Y. enterocolitica possessing the virF-, ail- and ystA-gene were isolated. Additionally, the ystB-gene was identified in 94.1% of the other Y. enterocolitica isolates, suggestive for biotype 1A. Three of these latter isolates simultaneously possessed the ail-virulence gene. Significantly more Y. enterocolitica were isolated during winter and spring compared to summer. Based on our findings we can conclude that brown rats are frequent carriers for various Yersinia spp., including Y. pseudotuberculosis and (human pathogenic) Y. enterocolitica which are more often isolated during winter and spring. Public Library of Science 2017-04-12 /pmc/articles/PMC5389835/ /pubmed/28403184 http://dx.doi.org/10.1371/journal.pone.0175648 Text en © 2017 Rouffaer et al http://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
spellingShingle Research Article
Rouffaer, Lieze Oscar
Baert, Kristof
Van den Abeele, Anne-Marie
Cox, Ivo
Vanantwerpen, Gerty
De Zutter, Lieven
Strubbe, Diederik
Vranckx, Katleen
Lens, Luc
Haesebrouck, Freddy
Delmée, Michel
Pasmans, Frank
Martel, An
Low prevalence of human enteropathogenic Yersinia spp. in brown rats (Rattus norvegicus) in Flanders
title Low prevalence of human enteropathogenic Yersinia spp. in brown rats (Rattus norvegicus) in Flanders
title_full Low prevalence of human enteropathogenic Yersinia spp. in brown rats (Rattus norvegicus) in Flanders
title_fullStr Low prevalence of human enteropathogenic Yersinia spp. in brown rats (Rattus norvegicus) in Flanders
title_full_unstemmed Low prevalence of human enteropathogenic Yersinia spp. in brown rats (Rattus norvegicus) in Flanders
title_short Low prevalence of human enteropathogenic Yersinia spp. in brown rats (Rattus norvegicus) in Flanders
title_sort low prevalence of human enteropathogenic yersinia spp. in brown rats (rattus norvegicus) in flanders
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5389835/
https://www.ncbi.nlm.nih.gov/pubmed/28403184
http://dx.doi.org/10.1371/journal.pone.0175648
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