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Functional analysis of the GmESR1 gene associated with soybean regeneration
Plant regeneration can occur via in vitro tissue culture through somatic embryogenesis or de novo shoot organogenesis. Transformation of soybean (Glycine max) is difficult, hence optimization of the transformation system for soybean regeneration is required. This study investigated ENHANCER OF SHOOT...
Autores principales: | , , , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Public Library of Science
2017
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5389854/ https://www.ncbi.nlm.nih.gov/pubmed/28403182 http://dx.doi.org/10.1371/journal.pone.0175656 |
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author | Zhang, Chao Wu, Xiaodong Zhang, Binbin Chen, Qingshan Liu, Ming Xin, Dawei Qi, Zhaoming Li, Sinan Ma, Yanlong Wang, Lingshuang Jin, Yangmei Li, Wenbin Wu, Xiaoxia Su, An-yu |
author_facet | Zhang, Chao Wu, Xiaodong Zhang, Binbin Chen, Qingshan Liu, Ming Xin, Dawei Qi, Zhaoming Li, Sinan Ma, Yanlong Wang, Lingshuang Jin, Yangmei Li, Wenbin Wu, Xiaoxia Su, An-yu |
author_sort | Zhang, Chao |
collection | PubMed |
description | Plant regeneration can occur via in vitro tissue culture through somatic embryogenesis or de novo shoot organogenesis. Transformation of soybean (Glycine max) is difficult, hence optimization of the transformation system for soybean regeneration is required. This study investigated ENHANCER OF SHOOT REGENERATION 1 (GmESR1), a soybean transcription factor that targets regeneration-associated genes. Sequence analysis showed that GmESR1 contained a conserved 57 amino acid APETALA 2 (AP2)/ETHYLENE RESPONSE FACTOR (ERF) DNA-binding domain. The relative expression level of GmESR1 was highest in young embryos, flowers and stems in the soybean cultivar ‘Dongnong 50’. To examine the function of GmESR1, transgenic Arabidopsis (Arabidopsis thaliana) and soybean plants overexpressing GmESR1 were generated. In Arabidopsis, overexpression of GmESR1 resulted in accelerated seed germination, and seedling shoot and root elongation. In soybean overexpression of GmESR1 also led to faster seed germination, and shoot and root elongation. GmESR1 specifically bound to the GCC-box. The results provide a foundation for the establishment of an efficient and stable transformation system for soybean. |
format | Online Article Text |
id | pubmed-5389854 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2017 |
publisher | Public Library of Science |
record_format | MEDLINE/PubMed |
spelling | pubmed-53898542017-05-03 Functional analysis of the GmESR1 gene associated with soybean regeneration Zhang, Chao Wu, Xiaodong Zhang, Binbin Chen, Qingshan Liu, Ming Xin, Dawei Qi, Zhaoming Li, Sinan Ma, Yanlong Wang, Lingshuang Jin, Yangmei Li, Wenbin Wu, Xiaoxia Su, An-yu PLoS One Research Article Plant regeneration can occur via in vitro tissue culture through somatic embryogenesis or de novo shoot organogenesis. Transformation of soybean (Glycine max) is difficult, hence optimization of the transformation system for soybean regeneration is required. This study investigated ENHANCER OF SHOOT REGENERATION 1 (GmESR1), a soybean transcription factor that targets regeneration-associated genes. Sequence analysis showed that GmESR1 contained a conserved 57 amino acid APETALA 2 (AP2)/ETHYLENE RESPONSE FACTOR (ERF) DNA-binding domain. The relative expression level of GmESR1 was highest in young embryos, flowers and stems in the soybean cultivar ‘Dongnong 50’. To examine the function of GmESR1, transgenic Arabidopsis (Arabidopsis thaliana) and soybean plants overexpressing GmESR1 were generated. In Arabidopsis, overexpression of GmESR1 resulted in accelerated seed germination, and seedling shoot and root elongation. In soybean overexpression of GmESR1 also led to faster seed germination, and shoot and root elongation. GmESR1 specifically bound to the GCC-box. The results provide a foundation for the establishment of an efficient and stable transformation system for soybean. Public Library of Science 2017-04-12 /pmc/articles/PMC5389854/ /pubmed/28403182 http://dx.doi.org/10.1371/journal.pone.0175656 Text en © 2017 Zhang et al http://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. |
spellingShingle | Research Article Zhang, Chao Wu, Xiaodong Zhang, Binbin Chen, Qingshan Liu, Ming Xin, Dawei Qi, Zhaoming Li, Sinan Ma, Yanlong Wang, Lingshuang Jin, Yangmei Li, Wenbin Wu, Xiaoxia Su, An-yu Functional analysis of the GmESR1 gene associated with soybean regeneration |
title | Functional analysis of the GmESR1 gene associated with soybean regeneration |
title_full | Functional analysis of the GmESR1 gene associated with soybean regeneration |
title_fullStr | Functional analysis of the GmESR1 gene associated with soybean regeneration |
title_full_unstemmed | Functional analysis of the GmESR1 gene associated with soybean regeneration |
title_short | Functional analysis of the GmESR1 gene associated with soybean regeneration |
title_sort | functional analysis of the gmesr1 gene associated with soybean regeneration |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5389854/ https://www.ncbi.nlm.nih.gov/pubmed/28403182 http://dx.doi.org/10.1371/journal.pone.0175656 |
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