Cargando…

Functional analysis of the GmESR1 gene associated with soybean regeneration

Plant regeneration can occur via in vitro tissue culture through somatic embryogenesis or de novo shoot organogenesis. Transformation of soybean (Glycine max) is difficult, hence optimization of the transformation system for soybean regeneration is required. This study investigated ENHANCER OF SHOOT...

Descripción completa

Detalles Bibliográficos
Autores principales: Zhang, Chao, Wu, Xiaodong, Zhang, Binbin, Chen, Qingshan, Liu, Ming, Xin, Dawei, Qi, Zhaoming, Li, Sinan, Ma, Yanlong, Wang, Lingshuang, Jin, Yangmei, Li, Wenbin, Wu, Xiaoxia, Su, An-yu
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5389854/
https://www.ncbi.nlm.nih.gov/pubmed/28403182
http://dx.doi.org/10.1371/journal.pone.0175656
_version_ 1782521350652428288
author Zhang, Chao
Wu, Xiaodong
Zhang, Binbin
Chen, Qingshan
Liu, Ming
Xin, Dawei
Qi, Zhaoming
Li, Sinan
Ma, Yanlong
Wang, Lingshuang
Jin, Yangmei
Li, Wenbin
Wu, Xiaoxia
Su, An-yu
author_facet Zhang, Chao
Wu, Xiaodong
Zhang, Binbin
Chen, Qingshan
Liu, Ming
Xin, Dawei
Qi, Zhaoming
Li, Sinan
Ma, Yanlong
Wang, Lingshuang
Jin, Yangmei
Li, Wenbin
Wu, Xiaoxia
Su, An-yu
author_sort Zhang, Chao
collection PubMed
description Plant regeneration can occur via in vitro tissue culture through somatic embryogenesis or de novo shoot organogenesis. Transformation of soybean (Glycine max) is difficult, hence optimization of the transformation system for soybean regeneration is required. This study investigated ENHANCER OF SHOOT REGENERATION 1 (GmESR1), a soybean transcription factor that targets regeneration-associated genes. Sequence analysis showed that GmESR1 contained a conserved 57 amino acid APETALA 2 (AP2)/ETHYLENE RESPONSE FACTOR (ERF) DNA-binding domain. The relative expression level of GmESR1 was highest in young embryos, flowers and stems in the soybean cultivar ‘Dongnong 50’. To examine the function of GmESR1, transgenic Arabidopsis (Arabidopsis thaliana) and soybean plants overexpressing GmESR1 were generated. In Arabidopsis, overexpression of GmESR1 resulted in accelerated seed germination, and seedling shoot and root elongation. In soybean overexpression of GmESR1 also led to faster seed germination, and shoot and root elongation. GmESR1 specifically bound to the GCC-box. The results provide a foundation for the establishment of an efficient and stable transformation system for soybean.
format Online
Article
Text
id pubmed-5389854
institution National Center for Biotechnology Information
language English
publishDate 2017
publisher Public Library of Science
record_format MEDLINE/PubMed
spelling pubmed-53898542017-05-03 Functional analysis of the GmESR1 gene associated with soybean regeneration Zhang, Chao Wu, Xiaodong Zhang, Binbin Chen, Qingshan Liu, Ming Xin, Dawei Qi, Zhaoming Li, Sinan Ma, Yanlong Wang, Lingshuang Jin, Yangmei Li, Wenbin Wu, Xiaoxia Su, An-yu PLoS One Research Article Plant regeneration can occur via in vitro tissue culture through somatic embryogenesis or de novo shoot organogenesis. Transformation of soybean (Glycine max) is difficult, hence optimization of the transformation system for soybean regeneration is required. This study investigated ENHANCER OF SHOOT REGENERATION 1 (GmESR1), a soybean transcription factor that targets regeneration-associated genes. Sequence analysis showed that GmESR1 contained a conserved 57 amino acid APETALA 2 (AP2)/ETHYLENE RESPONSE FACTOR (ERF) DNA-binding domain. The relative expression level of GmESR1 was highest in young embryos, flowers and stems in the soybean cultivar ‘Dongnong 50’. To examine the function of GmESR1, transgenic Arabidopsis (Arabidopsis thaliana) and soybean plants overexpressing GmESR1 were generated. In Arabidopsis, overexpression of GmESR1 resulted in accelerated seed germination, and seedling shoot and root elongation. In soybean overexpression of GmESR1 also led to faster seed germination, and shoot and root elongation. GmESR1 specifically bound to the GCC-box. The results provide a foundation for the establishment of an efficient and stable transformation system for soybean. Public Library of Science 2017-04-12 /pmc/articles/PMC5389854/ /pubmed/28403182 http://dx.doi.org/10.1371/journal.pone.0175656 Text en © 2017 Zhang et al http://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
spellingShingle Research Article
Zhang, Chao
Wu, Xiaodong
Zhang, Binbin
Chen, Qingshan
Liu, Ming
Xin, Dawei
Qi, Zhaoming
Li, Sinan
Ma, Yanlong
Wang, Lingshuang
Jin, Yangmei
Li, Wenbin
Wu, Xiaoxia
Su, An-yu
Functional analysis of the GmESR1 gene associated with soybean regeneration
title Functional analysis of the GmESR1 gene associated with soybean regeneration
title_full Functional analysis of the GmESR1 gene associated with soybean regeneration
title_fullStr Functional analysis of the GmESR1 gene associated with soybean regeneration
title_full_unstemmed Functional analysis of the GmESR1 gene associated with soybean regeneration
title_short Functional analysis of the GmESR1 gene associated with soybean regeneration
title_sort functional analysis of the gmesr1 gene associated with soybean regeneration
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5389854/
https://www.ncbi.nlm.nih.gov/pubmed/28403182
http://dx.doi.org/10.1371/journal.pone.0175656
work_keys_str_mv AT zhangchao functionalanalysisofthegmesr1geneassociatedwithsoybeanregeneration
AT wuxiaodong functionalanalysisofthegmesr1geneassociatedwithsoybeanregeneration
AT zhangbinbin functionalanalysisofthegmesr1geneassociatedwithsoybeanregeneration
AT chenqingshan functionalanalysisofthegmesr1geneassociatedwithsoybeanregeneration
AT liuming functionalanalysisofthegmesr1geneassociatedwithsoybeanregeneration
AT xindawei functionalanalysisofthegmesr1geneassociatedwithsoybeanregeneration
AT qizhaoming functionalanalysisofthegmesr1geneassociatedwithsoybeanregeneration
AT lisinan functionalanalysisofthegmesr1geneassociatedwithsoybeanregeneration
AT mayanlong functionalanalysisofthegmesr1geneassociatedwithsoybeanregeneration
AT wanglingshuang functionalanalysisofthegmesr1geneassociatedwithsoybeanregeneration
AT jinyangmei functionalanalysisofthegmesr1geneassociatedwithsoybeanregeneration
AT liwenbin functionalanalysisofthegmesr1geneassociatedwithsoybeanregeneration
AT wuxiaoxia functionalanalysisofthegmesr1geneassociatedwithsoybeanregeneration
AT suanyu functionalanalysisofthegmesr1geneassociatedwithsoybeanregeneration