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Super-resolution Microscopy – Applications in Plant Cell Research
Most of the present knowledge about cell organization and function is based on molecular and genetic methods as well as cytological investigations. While electron microscopy allows identifying cell substructures until a resolution of ∼1 nm, the resolution of fluorescence microscopy is restricted to...
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Formato: | Online Artículo Texto |
Lenguaje: | English |
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Frontiers Media S.A.
2017
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Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5390026/ https://www.ncbi.nlm.nih.gov/pubmed/28450874 http://dx.doi.org/10.3389/fpls.2017.00531 |
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author | Schubert, Veit |
author_facet | Schubert, Veit |
author_sort | Schubert, Veit |
collection | PubMed |
description | Most of the present knowledge about cell organization and function is based on molecular and genetic methods as well as cytological investigations. While electron microscopy allows identifying cell substructures until a resolution of ∼1 nm, the resolution of fluorescence microscopy is restricted to ∼200 nm due to the diffraction limit of light. However, the advantage of this technique is the possibility to identify and co-localize specifically labeled structures and molecules. The recently developed super-resolution microscopy techniques, such as Structured Illumination Microscopy, Photoactivated Localization Microscopy, Stochastic Optical Reconstruction Microscopy, and Stimulated Emission Depletion microscopy allow analyzing structures and molecules beyond the diffraction limit of light. Recently, there is an increasing application of these techniques in cell biology. This review evaluates and summarizes especially the data achieved until now in analyzing the organization and function of plant cells, chromosomes and interphase nuclei using super-resolution techniques. |
format | Online Article Text |
id | pubmed-5390026 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2017 |
publisher | Frontiers Media S.A. |
record_format | MEDLINE/PubMed |
spelling | pubmed-53900262017-04-27 Super-resolution Microscopy – Applications in Plant Cell Research Schubert, Veit Front Plant Sci Plant Science Most of the present knowledge about cell organization and function is based on molecular and genetic methods as well as cytological investigations. While electron microscopy allows identifying cell substructures until a resolution of ∼1 nm, the resolution of fluorescence microscopy is restricted to ∼200 nm due to the diffraction limit of light. However, the advantage of this technique is the possibility to identify and co-localize specifically labeled structures and molecules. The recently developed super-resolution microscopy techniques, such as Structured Illumination Microscopy, Photoactivated Localization Microscopy, Stochastic Optical Reconstruction Microscopy, and Stimulated Emission Depletion microscopy allow analyzing structures and molecules beyond the diffraction limit of light. Recently, there is an increasing application of these techniques in cell biology. This review evaluates and summarizes especially the data achieved until now in analyzing the organization and function of plant cells, chromosomes and interphase nuclei using super-resolution techniques. Frontiers Media S.A. 2017-04-13 /pmc/articles/PMC5390026/ /pubmed/28450874 http://dx.doi.org/10.3389/fpls.2017.00531 Text en Copyright © 2017 Schubert. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms. |
spellingShingle | Plant Science Schubert, Veit Super-resolution Microscopy – Applications in Plant Cell Research |
title | Super-resolution Microscopy – Applications in Plant Cell Research |
title_full | Super-resolution Microscopy – Applications in Plant Cell Research |
title_fullStr | Super-resolution Microscopy – Applications in Plant Cell Research |
title_full_unstemmed | Super-resolution Microscopy – Applications in Plant Cell Research |
title_short | Super-resolution Microscopy – Applications in Plant Cell Research |
title_sort | super-resolution microscopy – applications in plant cell research |
topic | Plant Science |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5390026/ https://www.ncbi.nlm.nih.gov/pubmed/28450874 http://dx.doi.org/10.3389/fpls.2017.00531 |
work_keys_str_mv | AT schubertveit superresolutionmicroscopyapplicationsinplantcellresearch |