Cross-talk between signaling pathways: The link between plant secondary metabolite production and wounding stress response

Plants subjected to wounding stress produce secondary metabolites. Several of these metabolites prevent chronic diseases and can be used as colorants, flavors, and as antimicrobials. This wound-induced production of plant secondary metabolites is mediated by signaling-molecules such as reactive oxyg...

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Autores principales: Jacobo-Velázquez, Daniel A., González-Agüero, Mauricio, Cisneros-Zevallos, Luis
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group 2015
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5390084/
https://www.ncbi.nlm.nih.gov/pubmed/25712739
http://dx.doi.org/10.1038/srep08608
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author Jacobo-Velázquez, Daniel A.
González-Agüero, Mauricio
Cisneros-Zevallos, Luis
author_facet Jacobo-Velázquez, Daniel A.
González-Agüero, Mauricio
Cisneros-Zevallos, Luis
author_sort Jacobo-Velázquez, Daniel A.
collection PubMed
description Plants subjected to wounding stress produce secondary metabolites. Several of these metabolites prevent chronic diseases and can be used as colorants, flavors, and as antimicrobials. This wound-induced production of plant secondary metabolites is mediated by signaling-molecules such as reactive oxygen species (ROS), ethylene (ET) and jasmonic acid (JA). However, their specific role and interactions that modulate the wound-respond in plants is not fully understood. In the present study, a subtractive cDNA library was generated, to better understand the global response of plants to wounding stress. Carrot (Daucus carota) was used as a model system for this study. A total of 335 unique expressed sequence tags (ESTs) sequences were obtained. ESTs sequences with a putative identity showed involvement in stress-signaling pathways as well as on the primary and secondary metabolism. Inhibitors of ROS biosynthesis, ET action, and JA biosynthesis alone and in combination were applied to wounded-carrots in order to determine, based on relative gene expression data, the regulatory role of ET, JA, and ROS on the wound-response in plants. Our results demonstrate that ROS play a key role as signaling-molecules for the wound-induced activation of the primary and secondary metabolism whereas ET and JA are essential to modulate ROS levels.
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spelling pubmed-53900842017-04-14 Cross-talk between signaling pathways: The link between plant secondary metabolite production and wounding stress response Jacobo-Velázquez, Daniel A. González-Agüero, Mauricio Cisneros-Zevallos, Luis Sci Rep Article Plants subjected to wounding stress produce secondary metabolites. Several of these metabolites prevent chronic diseases and can be used as colorants, flavors, and as antimicrobials. This wound-induced production of plant secondary metabolites is mediated by signaling-molecules such as reactive oxygen species (ROS), ethylene (ET) and jasmonic acid (JA). However, their specific role and interactions that modulate the wound-respond in plants is not fully understood. In the present study, a subtractive cDNA library was generated, to better understand the global response of plants to wounding stress. Carrot (Daucus carota) was used as a model system for this study. A total of 335 unique expressed sequence tags (ESTs) sequences were obtained. ESTs sequences with a putative identity showed involvement in stress-signaling pathways as well as on the primary and secondary metabolism. Inhibitors of ROS biosynthesis, ET action, and JA biosynthesis alone and in combination were applied to wounded-carrots in order to determine, based on relative gene expression data, the regulatory role of ET, JA, and ROS on the wound-response in plants. Our results demonstrate that ROS play a key role as signaling-molecules for the wound-induced activation of the primary and secondary metabolism whereas ET and JA are essential to modulate ROS levels. Nature Publishing Group 2015-02-25 /pmc/articles/PMC5390084/ /pubmed/25712739 http://dx.doi.org/10.1038/srep08608 Text en Copyright © 2015, Macmillan Publishers Limited. All rights reserved http://creativecommons.org/licenses/by/4.0/ This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article's Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder in order to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/
spellingShingle Article
Jacobo-Velázquez, Daniel A.
González-Agüero, Mauricio
Cisneros-Zevallos, Luis
Cross-talk between signaling pathways: The link between plant secondary metabolite production and wounding stress response
title Cross-talk between signaling pathways: The link between plant secondary metabolite production and wounding stress response
title_full Cross-talk between signaling pathways: The link between plant secondary metabolite production and wounding stress response
title_fullStr Cross-talk between signaling pathways: The link between plant secondary metabolite production and wounding stress response
title_full_unstemmed Cross-talk between signaling pathways: The link between plant secondary metabolite production and wounding stress response
title_short Cross-talk between signaling pathways: The link between plant secondary metabolite production and wounding stress response
title_sort cross-talk between signaling pathways: the link between plant secondary metabolite production and wounding stress response
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5390084/
https://www.ncbi.nlm.nih.gov/pubmed/25712739
http://dx.doi.org/10.1038/srep08608
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