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Revelation of mRNAs and proteins in porcine milk exosomes by transcriptomic and proteomic analysis

BACKGROUND: Milk is a complex liquid that provides nutrition to newborns. Recent reports demonstrated that milk is enriched in maternal-derived exosomes that are involved in fetal physiological and pathological conditions by transmission of exosomal mRNAs, miRNAs and proteins. Until now, there is no...

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Autores principales: Chen, Ting, Xi, Qian-Yun, Sun, Jia-Jie, Ye, Rui-Song, Cheng, Xiao, Sun, Rui-Ping, Wang, Song-Bo, Shu, Gang, Wang, Li-Na, Zhu, Xiao-Tong, Jiang, Qing-Yan, Zhang, Yong-Liang
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5390444/
https://www.ncbi.nlm.nih.gov/pubmed/28407805
http://dx.doi.org/10.1186/s12917-017-1021-8
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author Chen, Ting
Xi, Qian-Yun
Sun, Jia-Jie
Ye, Rui-Song
Cheng, Xiao
Sun, Rui-Ping
Wang, Song-Bo
Shu, Gang
Wang, Li-Na
Zhu, Xiao-Tong
Jiang, Qing-Yan
Zhang, Yong-Liang
author_facet Chen, Ting
Xi, Qian-Yun
Sun, Jia-Jie
Ye, Rui-Song
Cheng, Xiao
Sun, Rui-Ping
Wang, Song-Bo
Shu, Gang
Wang, Li-Na
Zhu, Xiao-Tong
Jiang, Qing-Yan
Zhang, Yong-Liang
author_sort Chen, Ting
collection PubMed
description BACKGROUND: Milk is a complex liquid that provides nutrition to newborns. Recent reports demonstrated that milk is enriched in maternal-derived exosomes that are involved in fetal physiological and pathological conditions by transmission of exosomal mRNAs, miRNAs and proteins. Until now, there is no such research relevant to exosomal mRNAs and proteins in porcine milk, therefore, we have attempted to investigate porcine milk exosomal mRNAs and proteins using RNA-sequencing and proteomic analysis. RESULTS: A total of 16,304 (13,895 known and 2,409 novel mRNAs) mRNAs and 639 (571 known, 66 candidate and 2 putative proteins) proteins were identified. GO and KEGG annotation indicated that most proteins were located in the cytoplasm and participated in many immunity and disease-related pathways, and some mRNAs were closely related to metabolisms, degradation and signaling pathways. Interestingly, 19 categories of proteins were tissue-specific and detected in placenta, liver, milk, plasma and mammary. COG analysis divided the identified mRNAs and proteins into 6 and 23 categories, respectively, 18 mRNAs and 10 proteins appeared to be involved in cell cycle control, cell division and chromosome partitioning. Additionally, 14 selected mRNAs were identified by qPCR, meanwhile, 10 proteins related to immunity and cell proliferation were detected by Western blot. CONCLUSIONS: These results provide the first insight into porcine milk exosomal mRNA and proteins, and will facilitate further research into the physiological significance of milk exosomes for infants. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (doi:10.1186/s12917-017-1021-8) contains supplementary material, which is available to authorized users.
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spelling pubmed-53904442017-04-14 Revelation of mRNAs and proteins in porcine milk exosomes by transcriptomic and proteomic analysis Chen, Ting Xi, Qian-Yun Sun, Jia-Jie Ye, Rui-Song Cheng, Xiao Sun, Rui-Ping Wang, Song-Bo Shu, Gang Wang, Li-Na Zhu, Xiao-Tong Jiang, Qing-Yan Zhang, Yong-Liang BMC Vet Res Research Article BACKGROUND: Milk is a complex liquid that provides nutrition to newborns. Recent reports demonstrated that milk is enriched in maternal-derived exosomes that are involved in fetal physiological and pathological conditions by transmission of exosomal mRNAs, miRNAs and proteins. Until now, there is no such research relevant to exosomal mRNAs and proteins in porcine milk, therefore, we have attempted to investigate porcine milk exosomal mRNAs and proteins using RNA-sequencing and proteomic analysis. RESULTS: A total of 16,304 (13,895 known and 2,409 novel mRNAs) mRNAs and 639 (571 known, 66 candidate and 2 putative proteins) proteins were identified. GO and KEGG annotation indicated that most proteins were located in the cytoplasm and participated in many immunity and disease-related pathways, and some mRNAs were closely related to metabolisms, degradation and signaling pathways. Interestingly, 19 categories of proteins were tissue-specific and detected in placenta, liver, milk, plasma and mammary. COG analysis divided the identified mRNAs and proteins into 6 and 23 categories, respectively, 18 mRNAs and 10 proteins appeared to be involved in cell cycle control, cell division and chromosome partitioning. Additionally, 14 selected mRNAs were identified by qPCR, meanwhile, 10 proteins related to immunity and cell proliferation were detected by Western blot. CONCLUSIONS: These results provide the first insight into porcine milk exosomal mRNA and proteins, and will facilitate further research into the physiological significance of milk exosomes for infants. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (doi:10.1186/s12917-017-1021-8) contains supplementary material, which is available to authorized users. BioMed Central 2017-04-13 /pmc/articles/PMC5390444/ /pubmed/28407805 http://dx.doi.org/10.1186/s12917-017-1021-8 Text en © The Author(s). 2017 Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.
spellingShingle Research Article
Chen, Ting
Xi, Qian-Yun
Sun, Jia-Jie
Ye, Rui-Song
Cheng, Xiao
Sun, Rui-Ping
Wang, Song-Bo
Shu, Gang
Wang, Li-Na
Zhu, Xiao-Tong
Jiang, Qing-Yan
Zhang, Yong-Liang
Revelation of mRNAs and proteins in porcine milk exosomes by transcriptomic and proteomic analysis
title Revelation of mRNAs and proteins in porcine milk exosomes by transcriptomic and proteomic analysis
title_full Revelation of mRNAs and proteins in porcine milk exosomes by transcriptomic and proteomic analysis
title_fullStr Revelation of mRNAs and proteins in porcine milk exosomes by transcriptomic and proteomic analysis
title_full_unstemmed Revelation of mRNAs and proteins in porcine milk exosomes by transcriptomic and proteomic analysis
title_short Revelation of mRNAs and proteins in porcine milk exosomes by transcriptomic and proteomic analysis
title_sort revelation of mrnas and proteins in porcine milk exosomes by transcriptomic and proteomic analysis
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5390444/
https://www.ncbi.nlm.nih.gov/pubmed/28407805
http://dx.doi.org/10.1186/s12917-017-1021-8
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