Layer-Specific Organization of Local Excitatory and Inhibitory Synaptic Connectivity in the Rat Presubiculum
The presubiculum is part of the parahippocampal spatial navigation system and contains head direction and grid cells upstream of the medial entorhinal cortex. This position within the parahippocampal cortex renders the presubiculum uniquely suited for analyzing the circuit requirements underlying th...
Autores principales: | , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Oxford University Press
2017
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5390487/ https://www.ncbi.nlm.nih.gov/pubmed/28334142 http://dx.doi.org/10.1093/cercor/bhx049 |
_version_ | 1782521470515150848 |
---|---|
author | Peng, Yangfan Barreda Tomás, Federico J. Klisch, Constantin Vida, Imre Geiger, Jörg R.P. |
author_facet | Peng, Yangfan Barreda Tomás, Federico J. Klisch, Constantin Vida, Imre Geiger, Jörg R.P. |
author_sort | Peng, Yangfan |
collection | PubMed |
description | The presubiculum is part of the parahippocampal spatial navigation system and contains head direction and grid cells upstream of the medial entorhinal cortex. This position within the parahippocampal cortex renders the presubiculum uniquely suited for analyzing the circuit requirements underlying the emergence of spatially tuned neuronal activity. To identify the local circuit properties, we analyzed the topology of synaptic connections between pyramidal cells and interneurons in all layers of the presubiculum by testing 4250 potential synaptic connections using multiple whole-cell recordings of up to 8 cells simultaneously. Network topology showed layer-specific organization of microcircuits consistent with the prevailing distinction of superficial and deep layers. While connections among pyramidal cells were almost absent in superficial layers, deep layers exhibited an excitatory connectivity of 3.9%. In contrast, synaptic connectivity for inhibition was higher in superficial layers though markedly lower than in other cortical areas. Finally, synaptic amplitudes of both excitatory and inhibitory connections showed log-normal distributions suggesting a nonrandom functional connectivity. In summary, our study provides new insights into the microcircuit organization of the presubiculum by revealing area- and layer-specific connectivity rules and sets new constraints for future models of the parahippocampal navigation system. |
format | Online Article Text |
id | pubmed-5390487 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2017 |
publisher | Oxford University Press |
record_format | MEDLINE/PubMed |
spelling | pubmed-53904872017-04-24 Layer-Specific Organization of Local Excitatory and Inhibitory Synaptic Connectivity in the Rat Presubiculum Peng, Yangfan Barreda Tomás, Federico J. Klisch, Constantin Vida, Imre Geiger, Jörg R.P. Cereb Cortex Original Articles The presubiculum is part of the parahippocampal spatial navigation system and contains head direction and grid cells upstream of the medial entorhinal cortex. This position within the parahippocampal cortex renders the presubiculum uniquely suited for analyzing the circuit requirements underlying the emergence of spatially tuned neuronal activity. To identify the local circuit properties, we analyzed the topology of synaptic connections between pyramidal cells and interneurons in all layers of the presubiculum by testing 4250 potential synaptic connections using multiple whole-cell recordings of up to 8 cells simultaneously. Network topology showed layer-specific organization of microcircuits consistent with the prevailing distinction of superficial and deep layers. While connections among pyramidal cells were almost absent in superficial layers, deep layers exhibited an excitatory connectivity of 3.9%. In contrast, synaptic connectivity for inhibition was higher in superficial layers though markedly lower than in other cortical areas. Finally, synaptic amplitudes of both excitatory and inhibitory connections showed log-normal distributions suggesting a nonrandom functional connectivity. In summary, our study provides new insights into the microcircuit organization of the presubiculum by revealing area- and layer-specific connectivity rules and sets new constraints for future models of the parahippocampal navigation system. Oxford University Press 2017-04 2017-02-25 /pmc/articles/PMC5390487/ /pubmed/28334142 http://dx.doi.org/10.1093/cercor/bhx049 Text en © The Author 2017. Published by Oxford University Press. http://creativecommons.org/licenses/by-nc/4.0/ This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/4.0/), which permits non-commercial re-use, distribution, and reproduction in any medium, provided the original work is properly cited. For commercial re-use, please contact journals.permissions@oup.com |
spellingShingle | Original Articles Peng, Yangfan Barreda Tomás, Federico J. Klisch, Constantin Vida, Imre Geiger, Jörg R.P. Layer-Specific Organization of Local Excitatory and Inhibitory Synaptic Connectivity in the Rat Presubiculum |
title | Layer-Specific Organization of Local Excitatory and Inhibitory Synaptic Connectivity in the Rat Presubiculum |
title_full | Layer-Specific Organization of Local Excitatory and Inhibitory Synaptic Connectivity in the Rat Presubiculum |
title_fullStr | Layer-Specific Organization of Local Excitatory and Inhibitory Synaptic Connectivity in the Rat Presubiculum |
title_full_unstemmed | Layer-Specific Organization of Local Excitatory and Inhibitory Synaptic Connectivity in the Rat Presubiculum |
title_short | Layer-Specific Organization of Local Excitatory and Inhibitory Synaptic Connectivity in the Rat Presubiculum |
title_sort | layer-specific organization of local excitatory and inhibitory synaptic connectivity in the rat presubiculum |
topic | Original Articles |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5390487/ https://www.ncbi.nlm.nih.gov/pubmed/28334142 http://dx.doi.org/10.1093/cercor/bhx049 |
work_keys_str_mv | AT pengyangfan layerspecificorganizationoflocalexcitatoryandinhibitorysynapticconnectivityintheratpresubiculum AT barredatomasfedericoj layerspecificorganizationoflocalexcitatoryandinhibitorysynapticconnectivityintheratpresubiculum AT klischconstantin layerspecificorganizationoflocalexcitatoryandinhibitorysynapticconnectivityintheratpresubiculum AT vidaimre layerspecificorganizationoflocalexcitatoryandinhibitorysynapticconnectivityintheratpresubiculum AT geigerjorgrp layerspecificorganizationoflocalexcitatoryandinhibitorysynapticconnectivityintheratpresubiculum |