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Recycle of Immobilized Endocellulases in Different Conditions for Cellulose Hydrolysis

The immobilization of cellulases could be an economical alternative for cost reduction of enzyme application. The derivatives obtained in the immobilization derivatives were evaluated in recycles of paper filter hydrolysis. The immobilization process showed that the enzyme recycles were influenced b...

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Autores principales: Silva, D. F., Carvalho, A. F. A., Shinya, T. Y., Mazali, G. S., Herculano, R. D., Oliva-Neto, P.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Hindawi 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5390571/
https://www.ncbi.nlm.nih.gov/pubmed/28465836
http://dx.doi.org/10.1155/2017/4362704
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author Silva, D. F.
Carvalho, A. F. A.
Shinya, T. Y.
Mazali, G. S.
Herculano, R. D.
Oliva-Neto, P.
author_facet Silva, D. F.
Carvalho, A. F. A.
Shinya, T. Y.
Mazali, G. S.
Herculano, R. D.
Oliva-Neto, P.
author_sort Silva, D. F.
collection PubMed
description The immobilization of cellulases could be an economical alternative for cost reduction of enzyme application. The derivatives obtained in the immobilization derivatives were evaluated in recycles of paper filter hydrolysis. The immobilization process showed that the enzyme recycles were influenced by the shape (drop or sheet) and type of the mixture. The enzyme was recycled 28 times for sheets E′ and 13 times for drops B′. The derivative E′ showed the highest stability in the recycle obtaining 0.05 FPU/g, RA of 10%, and FPU Yield of 1.64 times, higher than FPU spent or Net FPU Yield of 5.3 times, saving more active enzymes. The derivative B showed stability in recycles reaching 0.15 FPU/g of derivative, yield of Recovered Activity (RA) of 25%, and FPU Yield of 1.57 times, higher than FPU spent on immobilization or Net PFU Yield of 2.81 times. The latex increased stability and resistance of the drops but did not improve the FPU/gram of derivative.
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spelling pubmed-53905712017-05-02 Recycle of Immobilized Endocellulases in Different Conditions for Cellulose Hydrolysis Silva, D. F. Carvalho, A. F. A. Shinya, T. Y. Mazali, G. S. Herculano, R. D. Oliva-Neto, P. Enzyme Res Research Article The immobilization of cellulases could be an economical alternative for cost reduction of enzyme application. The derivatives obtained in the immobilization derivatives were evaluated in recycles of paper filter hydrolysis. The immobilization process showed that the enzyme recycles were influenced by the shape (drop or sheet) and type of the mixture. The enzyme was recycled 28 times for sheets E′ and 13 times for drops B′. The derivative E′ showed the highest stability in the recycle obtaining 0.05 FPU/g, RA of 10%, and FPU Yield of 1.64 times, higher than FPU spent or Net FPU Yield of 5.3 times, saving more active enzymes. The derivative B showed stability in recycles reaching 0.15 FPU/g of derivative, yield of Recovered Activity (RA) of 25%, and FPU Yield of 1.57 times, higher than FPU spent on immobilization or Net PFU Yield of 2.81 times. The latex increased stability and resistance of the drops but did not improve the FPU/gram of derivative. Hindawi 2017 2017-03-29 /pmc/articles/PMC5390571/ /pubmed/28465836 http://dx.doi.org/10.1155/2017/4362704 Text en Copyright © 2017 D. F. Silva et al. https://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Silva, D. F.
Carvalho, A. F. A.
Shinya, T. Y.
Mazali, G. S.
Herculano, R. D.
Oliva-Neto, P.
Recycle of Immobilized Endocellulases in Different Conditions for Cellulose Hydrolysis
title Recycle of Immobilized Endocellulases in Different Conditions for Cellulose Hydrolysis
title_full Recycle of Immobilized Endocellulases in Different Conditions for Cellulose Hydrolysis
title_fullStr Recycle of Immobilized Endocellulases in Different Conditions for Cellulose Hydrolysis
title_full_unstemmed Recycle of Immobilized Endocellulases in Different Conditions for Cellulose Hydrolysis
title_short Recycle of Immobilized Endocellulases in Different Conditions for Cellulose Hydrolysis
title_sort recycle of immobilized endocellulases in different conditions for cellulose hydrolysis
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5390571/
https://www.ncbi.nlm.nih.gov/pubmed/28465836
http://dx.doi.org/10.1155/2017/4362704
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