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Thiamine Assays—Advances, Challenges, and Caveats

Thiamine (vitamin B1) is essential to the health of all living organisms and deficiency has long been associated with diseases in animals such as fish, birds, alligators, and domesticated ruminant mammals. Thiamine is also implicated in several human diseases including Alzheimer's, diabetes, de...

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Autores principales: Edwards, Katie A., Tu‐Maung, Nicole, Cheng, Krystal, Wang, Binbin, Baeumner, Antje J., Kraft, Clifford E.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: John Wiley and Sons Inc. 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5390807/
https://www.ncbi.nlm.nih.gov/pubmed/28413748
http://dx.doi.org/10.1002/open.201600160
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author Edwards, Katie A.
Tu‐Maung, Nicole
Cheng, Krystal
Wang, Binbin
Baeumner, Antje J.
Kraft, Clifford E.
author_facet Edwards, Katie A.
Tu‐Maung, Nicole
Cheng, Krystal
Wang, Binbin
Baeumner, Antje J.
Kraft, Clifford E.
author_sort Edwards, Katie A.
collection PubMed
description Thiamine (vitamin B1) is essential to the health of all living organisms and deficiency has long been associated with diseases in animals such as fish, birds, alligators, and domesticated ruminant mammals. Thiamine is also implicated in several human diseases including Alzheimer's, diabetes, dementia, depression and, most notably, Wernicke–Korsakoff syndrome and Beriberi disease. Yet, highly sensitive and specific detection of thiamine remains an analytical challenge, as pM to nm levels of thiamine need to be detected in environmental and human samples, respectively, various phosphorylated variants need to be discriminated, and rapid on‐site detection would be highly desirable. Furthermore, appropriate sample preparation is mandatory, owing to the complexity of the relevant sample matrices including fish tissues, ocean water, and body fluids. This Review has two objectives. First, it provides a thorough overview of analytical techniques published for thiamine detection over the last 15 years. Second, it describes the principles of analytical approaches that are based on biorecognition and may open up new avenues for rapid and high‐throughput thiamine analysis. Most notably, periplasmic binding proteins, ribozymes, and aptamers are of particular interest, as they function as bioaffinity recognition elements that can fill an important assay technology gap, owing to the unavailability of thiamine‐specific commercial antibodies. Finally, the authors provide brief evaluations of key outcomes of the major assay concepts and suggest how innovative techniques could help develop sensitive and specific thiamine analytical test systems.
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spelling pubmed-53908072017-04-14 Thiamine Assays—Advances, Challenges, and Caveats Edwards, Katie A. Tu‐Maung, Nicole Cheng, Krystal Wang, Binbin Baeumner, Antje J. Kraft, Clifford E. ChemistryOpen Reviews Thiamine (vitamin B1) is essential to the health of all living organisms and deficiency has long been associated with diseases in animals such as fish, birds, alligators, and domesticated ruminant mammals. Thiamine is also implicated in several human diseases including Alzheimer's, diabetes, dementia, depression and, most notably, Wernicke–Korsakoff syndrome and Beriberi disease. Yet, highly sensitive and specific detection of thiamine remains an analytical challenge, as pM to nm levels of thiamine need to be detected in environmental and human samples, respectively, various phosphorylated variants need to be discriminated, and rapid on‐site detection would be highly desirable. Furthermore, appropriate sample preparation is mandatory, owing to the complexity of the relevant sample matrices including fish tissues, ocean water, and body fluids. This Review has two objectives. First, it provides a thorough overview of analytical techniques published for thiamine detection over the last 15 years. Second, it describes the principles of analytical approaches that are based on biorecognition and may open up new avenues for rapid and high‐throughput thiamine analysis. Most notably, periplasmic binding proteins, ribozymes, and aptamers are of particular interest, as they function as bioaffinity recognition elements that can fill an important assay technology gap, owing to the unavailability of thiamine‐specific commercial antibodies. Finally, the authors provide brief evaluations of key outcomes of the major assay concepts and suggest how innovative techniques could help develop sensitive and specific thiamine analytical test systems. John Wiley and Sons Inc. 2017-03-08 /pmc/articles/PMC5390807/ /pubmed/28413748 http://dx.doi.org/10.1002/open.201600160 Text en © 2017 The Authors. Published by Wiley-VCH Verlag GmbH & Co. KGaA. This is an open access article under the terms of the Creative Commons Attribution‐NonCommercial‐NoDerivs (http://creativecommons.org/licenses/by-nc-nd/4.0/) License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non‐commercial and no modifications or adaptations are made.
spellingShingle Reviews
Edwards, Katie A.
Tu‐Maung, Nicole
Cheng, Krystal
Wang, Binbin
Baeumner, Antje J.
Kraft, Clifford E.
Thiamine Assays—Advances, Challenges, and Caveats
title Thiamine Assays—Advances, Challenges, and Caveats
title_full Thiamine Assays—Advances, Challenges, and Caveats
title_fullStr Thiamine Assays—Advances, Challenges, and Caveats
title_full_unstemmed Thiamine Assays—Advances, Challenges, and Caveats
title_short Thiamine Assays—Advances, Challenges, and Caveats
title_sort thiamine assays—advances, challenges, and caveats
topic Reviews
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5390807/
https://www.ncbi.nlm.nih.gov/pubmed/28413748
http://dx.doi.org/10.1002/open.201600160
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