Enhanced saccharification of lignocellulosic agricultural biomass and increased bioethanol titre using acclimated Clostridium thermocellum DSM1313

Consolidated bioprocess assures an efficient lignocellulosic conversion to fermentable sugars and subsequently to bioethanol. Such a single-step hydrolysis and anaerobic fermentation was achieved with acclimated Clostridium thermocellum DSM 1313 on different mildly pre-treated agricultural lignocellulosic residues without any additional enzymes/and strains. Acclimation was achieved by serially sub-culturing in increasing concentration of individual substrates, such as rice husk, sugarcane bagasse, and banana pseudostem in the standard media, with cellobiose as an adjunct. The acclimated cellulolytic thermophile exhibited an early log phase entry with enhanced growth compared to the direct inoculation experiments with unacclimated culture. Around 672 mg/g of reducing sugar was produced from sugarcane bagasse media and 636 mg/g from rice husk media and 513 mg/g from banana pseudostem media with the acclimated organism. Bioethanol production also doubled in experiments with serially acclimated cultures, with a maximum of 1.21 and 1.0 g/L ethanol titre from sugarcane bagasse and rice husk, respectively. The serial acclimation experiments have increased the saccharification potentials of the organism towards the respective lignocellulosic substrates and also enhanced the bioethanol production.