Cargando…
Vacuolar ion channels in the liverwort Marchantia polymorpha: influence of ion channel inhibitors
Potassium-permeable slow activating vacuolar channels (SV) and chloride-permeable channels in the vacuole of the liverwort Marchantia polymorpha were characterized in respect to calcium dependence, selectivity, and pharmacology. The patch-clamp method was used in the study of ion channel activity in...
Autores principales: | , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Springer Berlin Heidelberg
2017
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5391376/ https://www.ncbi.nlm.nih.gov/pubmed/28197715 http://dx.doi.org/10.1007/s00425-017-2661-4 |
_version_ | 1783229257047080960 |
---|---|
author | Koselski, Mateusz Trebacz, Kazimierz Dziubinska, Halina |
author_facet | Koselski, Mateusz Trebacz, Kazimierz Dziubinska, Halina |
author_sort | Koselski, Mateusz |
collection | PubMed |
description | Potassium-permeable slow activating vacuolar channels (SV) and chloride-permeable channels in the vacuole of the liverwort Marchantia polymorpha were characterized in respect to calcium dependence, selectivity, and pharmacology. The patch-clamp method was used in the study of ion channel activity in the vacuoles from the liverwort Marchantia polymorpha. The whole-vacuole recordings allowed simultaneous observation of two types of currents—predominant slow activated currents recorded at positive voltages and fast activated currents recorded at negative voltages. Single-channel recordings carried out in the gradient of KCl indicated that slow activated currents were carried by potassium-permeable slowly activating vacuolar channels (SV) and fast activated currents—by chloride-permeable channels. Both types of the channels were dependent in an opposite way on calcium, since elimination of this ion from the cytoplasmic side caused inhibition of SV channels, but the open probability of chloride-permeable channels even increased. The dependence of the activity of both channels on different types of ion channel inhibitors was studied. SV channels exhibited different sensitivity to potassium channel inhibitors. These channels were insensitive to 3 mM Ba(2+), but were blocked by 3 mM tetraethyl ammonium (TEA). Moreover, the activity of the channels was modified in a different way by calcium channel inhibitors. 200 µM Gd(3+) was an effective blocker, but 50 µM ruthenium red evoked bursts of the channel activity resulting in an increase in the open probability. Different effectiveness of anion channel inhibitors was observed in chloride-permeable channels. After the application of 100 µM Zn(2+), a decrease in the open probability was recorded but the channels were still active. 50 µM DIDS was more effective, as it completely blocked the channels. |
format | Online Article Text |
id | pubmed-5391376 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2017 |
publisher | Springer Berlin Heidelberg |
record_format | MEDLINE/PubMed |
spelling | pubmed-53913762017-04-28 Vacuolar ion channels in the liverwort Marchantia polymorpha: influence of ion channel inhibitors Koselski, Mateusz Trebacz, Kazimierz Dziubinska, Halina Planta Original Article Potassium-permeable slow activating vacuolar channels (SV) and chloride-permeable channels in the vacuole of the liverwort Marchantia polymorpha were characterized in respect to calcium dependence, selectivity, and pharmacology. The patch-clamp method was used in the study of ion channel activity in the vacuoles from the liverwort Marchantia polymorpha. The whole-vacuole recordings allowed simultaneous observation of two types of currents—predominant slow activated currents recorded at positive voltages and fast activated currents recorded at negative voltages. Single-channel recordings carried out in the gradient of KCl indicated that slow activated currents were carried by potassium-permeable slowly activating vacuolar channels (SV) and fast activated currents—by chloride-permeable channels. Both types of the channels were dependent in an opposite way on calcium, since elimination of this ion from the cytoplasmic side caused inhibition of SV channels, but the open probability of chloride-permeable channels even increased. The dependence of the activity of both channels on different types of ion channel inhibitors was studied. SV channels exhibited different sensitivity to potassium channel inhibitors. These channels were insensitive to 3 mM Ba(2+), but were blocked by 3 mM tetraethyl ammonium (TEA). Moreover, the activity of the channels was modified in a different way by calcium channel inhibitors. 200 µM Gd(3+) was an effective blocker, but 50 µM ruthenium red evoked bursts of the channel activity resulting in an increase in the open probability. Different effectiveness of anion channel inhibitors was observed in chloride-permeable channels. After the application of 100 µM Zn(2+), a decrease in the open probability was recorded but the channels were still active. 50 µM DIDS was more effective, as it completely blocked the channels. Springer Berlin Heidelberg 2017-02-14 2017 /pmc/articles/PMC5391376/ /pubmed/28197715 http://dx.doi.org/10.1007/s00425-017-2661-4 Text en © The Author(s) 2017 Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. |
spellingShingle | Original Article Koselski, Mateusz Trebacz, Kazimierz Dziubinska, Halina Vacuolar ion channels in the liverwort Marchantia polymorpha: influence of ion channel inhibitors |
title | Vacuolar ion channels in the liverwort Marchantia polymorpha: influence of ion channel inhibitors |
title_full | Vacuolar ion channels in the liverwort Marchantia polymorpha: influence of ion channel inhibitors |
title_fullStr | Vacuolar ion channels in the liverwort Marchantia polymorpha: influence of ion channel inhibitors |
title_full_unstemmed | Vacuolar ion channels in the liverwort Marchantia polymorpha: influence of ion channel inhibitors |
title_short | Vacuolar ion channels in the liverwort Marchantia polymorpha: influence of ion channel inhibitors |
title_sort | vacuolar ion channels in the liverwort marchantia polymorpha: influence of ion channel inhibitors |
topic | Original Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5391376/ https://www.ncbi.nlm.nih.gov/pubmed/28197715 http://dx.doi.org/10.1007/s00425-017-2661-4 |
work_keys_str_mv | AT koselskimateusz vacuolarionchannelsintheliverwortmarchantiapolymorphainfluenceofionchannelinhibitors AT trebaczkazimierz vacuolarionchannelsintheliverwortmarchantiapolymorphainfluenceofionchannelinhibitors AT dziubinskahalina vacuolarionchannelsintheliverwortmarchantiapolymorphainfluenceofionchannelinhibitors |