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Polarized proton spin density images the tyrosyl radical locations in bovine liver catalase

A tyrosyl radical, as part of the amino acid chain of bovine liver catalase, supports dynamic proton spin polarization (DNP). Finding the position of the tyrosyl radical within the macromolecule relies on the accumulation of proton polarization close to it, which is readily observed by polarized neu...

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Detalles Bibliográficos
Autores principales: Zimmer, Oliver, Jouve, Hélène M., Stuhrmann, Heinrich B.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: International Union of Crystallography 2016
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5391855/
https://www.ncbi.nlm.nih.gov/pubmed/28461894
http://dx.doi.org/10.1107/S205225251601054X
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author Zimmer, Oliver
Jouve, Hélène M.
Stuhrmann, Heinrich B.
author_facet Zimmer, Oliver
Jouve, Hélène M.
Stuhrmann, Heinrich B.
author_sort Zimmer, Oliver
collection PubMed
description A tyrosyl radical, as part of the amino acid chain of bovine liver catalase, supports dynamic proton spin polarization (DNP). Finding the position of the tyrosyl radical within the macromolecule relies on the accumulation of proton polarization close to it, which is readily observed by polarized neutron scattering. The nuclear scattering amplitude due to the polarization of protons less than 10 Å distant from the tyrosyl radical is ten times larger than the amplitude of magnetic neutron scattering from an unpaired polarized electron of the same radical. The direction of DNP was inverted every 5 s, and the initial evolution of the intensity of polarized neutron scattering after each inversion was used to identify those tyrosines which have assumed a radical state. Three radical sites, all of them close to the molecular centre and the haem, appear to be equally possible. Among these is tyr-369, the radical state of which had previously been proven by electron paramagnetic resonance.
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spelling pubmed-53918552017-05-01 Polarized proton spin density images the tyrosyl radical locations in bovine liver catalase Zimmer, Oliver Jouve, Hélène M. Stuhrmann, Heinrich B. IUCrJ Research Papers A tyrosyl radical, as part of the amino acid chain of bovine liver catalase, supports dynamic proton spin polarization (DNP). Finding the position of the tyrosyl radical within the macromolecule relies on the accumulation of proton polarization close to it, which is readily observed by polarized neutron scattering. The nuclear scattering amplitude due to the polarization of protons less than 10 Å distant from the tyrosyl radical is ten times larger than the amplitude of magnetic neutron scattering from an unpaired polarized electron of the same radical. The direction of DNP was inverted every 5 s, and the initial evolution of the intensity of polarized neutron scattering after each inversion was used to identify those tyrosines which have assumed a radical state. Three radical sites, all of them close to the molecular centre and the haem, appear to be equally possible. Among these is tyr-369, the radical state of which had previously been proven by electron paramagnetic resonance. International Union of Crystallography 2016-07-29 /pmc/articles/PMC5391855/ /pubmed/28461894 http://dx.doi.org/10.1107/S205225251601054X Text en © Oliver Zimmer et al. 2016 http://creativecommons.org/licenses/by/2.0/uk/ This is an open-access article distributed under the terms of the Creative Commons Attribution Licence, which permits unrestricted use, distribution, and reproduction in any medium, provided the original authors and source are cited.
spellingShingle Research Papers
Zimmer, Oliver
Jouve, Hélène M.
Stuhrmann, Heinrich B.
Polarized proton spin density images the tyrosyl radical locations in bovine liver catalase
title Polarized proton spin density images the tyrosyl radical locations in bovine liver catalase
title_full Polarized proton spin density images the tyrosyl radical locations in bovine liver catalase
title_fullStr Polarized proton spin density images the tyrosyl radical locations in bovine liver catalase
title_full_unstemmed Polarized proton spin density images the tyrosyl radical locations in bovine liver catalase
title_short Polarized proton spin density images the tyrosyl radical locations in bovine liver catalase
title_sort polarized proton spin density images the tyrosyl radical locations in bovine liver catalase
topic Research Papers
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5391855/
https://www.ncbi.nlm.nih.gov/pubmed/28461894
http://dx.doi.org/10.1107/S205225251601054X
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