Cargando…

Tuber indicum shapes the microbial communities of ectomycorhizosphere soil and ectomycorrhizae of an indigenous tree (Pinus armandii)

The aim of this study was to investigate the effect of an ectomycorrhizal fungus (Tuber indicum) on the diversity of microbial communities associated with an indigenous tree, Pinus armandii, and the microbial communities in the surrounding ectomycorhizosphere soil. High-throughput sequencing was use...

Descripción completa

Detalles Bibliográficos
Autores principales: Li, Qiang, Zhao, Jian, Xiong, Chuan, Li, Xiaolin, Chen, Zuqin, Li, Ping, Huang, Wenli
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5391931/
https://www.ncbi.nlm.nih.gov/pubmed/28410376
http://dx.doi.org/10.1371/journal.pone.0175720
_version_ 1783229365263269888
author Li, Qiang
Zhao, Jian
Xiong, Chuan
Li, Xiaolin
Chen, Zuqin
Li, Ping
Huang, Wenli
author_facet Li, Qiang
Zhao, Jian
Xiong, Chuan
Li, Xiaolin
Chen, Zuqin
Li, Ping
Huang, Wenli
author_sort Li, Qiang
collection PubMed
description The aim of this study was to investigate the effect of an ectomycorrhizal fungus (Tuber indicum) on the diversity of microbial communities associated with an indigenous tree, Pinus armandii, and the microbial communities in the surrounding ectomycorhizosphere soil. High-throughput sequencing was used to analyze the richness of microbial communities in the roots or rhizosphere of treatments with or without ectomycorrhizae. The results indicated that the bacterial diversity of ectomycorhizosphere soil was significantly lower compared with the control soil. Presumably, the dominance of truffle mycelia in ectomycorhizosphere soil (80.91%) and ectomycorrhizae (97.64%) was the main factor that resulted in lower diversity and abundance of endophytic pathogenic fungi, including Fusarium, Monographella, Ustilago and Rhizopus and other competitive mycorrhizal fungi, such as Amanita, Lactarius and Boletus. Bacterial genera Reyranena, Rhizomicrobium, Nordella, Pseudomonas and fungal genera, Cuphophyllus, Leucangium, Histoplasma were significantly more abundant in ectomycorrhizosphere soil and ectomycorrhizae. Hierarchical cluster analysis of the similarities between rhizosphere and ectomycorrhizosphere soil based on the soil properties differed significantly, indicating the mycorrhizal synthesis may have a feedback effect on soil properties. Meanwhile, some soil properties were significantly correlated with bacterial and fungal diversity in the rhizosphere or root tips. Overall, this work illustrates the interactive network that exists among ectomycorrhizal fungi, soil properties and microbial communities associated with the host plant and furthers our understanding of the ecology and cultivation of T. indicum.
format Online
Article
Text
id pubmed-5391931
institution National Center for Biotechnology Information
language English
publishDate 2017
publisher Public Library of Science
record_format MEDLINE/PubMed
spelling pubmed-53919312017-05-03 Tuber indicum shapes the microbial communities of ectomycorhizosphere soil and ectomycorrhizae of an indigenous tree (Pinus armandii) Li, Qiang Zhao, Jian Xiong, Chuan Li, Xiaolin Chen, Zuqin Li, Ping Huang, Wenli PLoS One Research Article The aim of this study was to investigate the effect of an ectomycorrhizal fungus (Tuber indicum) on the diversity of microbial communities associated with an indigenous tree, Pinus armandii, and the microbial communities in the surrounding ectomycorhizosphere soil. High-throughput sequencing was used to analyze the richness of microbial communities in the roots or rhizosphere of treatments with or without ectomycorrhizae. The results indicated that the bacterial diversity of ectomycorhizosphere soil was significantly lower compared with the control soil. Presumably, the dominance of truffle mycelia in ectomycorhizosphere soil (80.91%) and ectomycorrhizae (97.64%) was the main factor that resulted in lower diversity and abundance of endophytic pathogenic fungi, including Fusarium, Monographella, Ustilago and Rhizopus and other competitive mycorrhizal fungi, such as Amanita, Lactarius and Boletus. Bacterial genera Reyranena, Rhizomicrobium, Nordella, Pseudomonas and fungal genera, Cuphophyllus, Leucangium, Histoplasma were significantly more abundant in ectomycorrhizosphere soil and ectomycorrhizae. Hierarchical cluster analysis of the similarities between rhizosphere and ectomycorrhizosphere soil based on the soil properties differed significantly, indicating the mycorrhizal synthesis may have a feedback effect on soil properties. Meanwhile, some soil properties were significantly correlated with bacterial and fungal diversity in the rhizosphere or root tips. Overall, this work illustrates the interactive network that exists among ectomycorrhizal fungi, soil properties and microbial communities associated with the host plant and furthers our understanding of the ecology and cultivation of T. indicum. Public Library of Science 2017-04-14 /pmc/articles/PMC5391931/ /pubmed/28410376 http://dx.doi.org/10.1371/journal.pone.0175720 Text en © 2017 Li et al http://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
spellingShingle Research Article
Li, Qiang
Zhao, Jian
Xiong, Chuan
Li, Xiaolin
Chen, Zuqin
Li, Ping
Huang, Wenli
Tuber indicum shapes the microbial communities of ectomycorhizosphere soil and ectomycorrhizae of an indigenous tree (Pinus armandii)
title Tuber indicum shapes the microbial communities of ectomycorhizosphere soil and ectomycorrhizae of an indigenous tree (Pinus armandii)
title_full Tuber indicum shapes the microbial communities of ectomycorhizosphere soil and ectomycorrhizae of an indigenous tree (Pinus armandii)
title_fullStr Tuber indicum shapes the microbial communities of ectomycorhizosphere soil and ectomycorrhizae of an indigenous tree (Pinus armandii)
title_full_unstemmed Tuber indicum shapes the microbial communities of ectomycorhizosphere soil and ectomycorrhizae of an indigenous tree (Pinus armandii)
title_short Tuber indicum shapes the microbial communities of ectomycorhizosphere soil and ectomycorrhizae of an indigenous tree (Pinus armandii)
title_sort tuber indicum shapes the microbial communities of ectomycorhizosphere soil and ectomycorrhizae of an indigenous tree (pinus armandii)
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5391931/
https://www.ncbi.nlm.nih.gov/pubmed/28410376
http://dx.doi.org/10.1371/journal.pone.0175720
work_keys_str_mv AT liqiang tuberindicumshapesthemicrobialcommunitiesofectomycorhizospheresoilandectomycorrhizaeofanindigenoustreepinusarmandii
AT zhaojian tuberindicumshapesthemicrobialcommunitiesofectomycorhizospheresoilandectomycorrhizaeofanindigenoustreepinusarmandii
AT xiongchuan tuberindicumshapesthemicrobialcommunitiesofectomycorhizospheresoilandectomycorrhizaeofanindigenoustreepinusarmandii
AT lixiaolin tuberindicumshapesthemicrobialcommunitiesofectomycorhizospheresoilandectomycorrhizaeofanindigenoustreepinusarmandii
AT chenzuqin tuberindicumshapesthemicrobialcommunitiesofectomycorhizospheresoilandectomycorrhizaeofanindigenoustreepinusarmandii
AT liping tuberindicumshapesthemicrobialcommunitiesofectomycorhizospheresoilandectomycorrhizaeofanindigenoustreepinusarmandii
AT huangwenli tuberindicumshapesthemicrobialcommunitiesofectomycorhizospheresoilandectomycorrhizaeofanindigenoustreepinusarmandii