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Accessory gene regulator (Agr) functionality in Staphylococcus aureus derived from lower respiratory tract infections
OBJECTIVE: Characterization of Staphylococcus aureus clinical isolates derived from lower respiratory tract infections (LRTIs), and correlation between the functionality of the accessory gene regulator (Agr) and genotypic and phenotypic characteristics, clinical variables and clinical outcome. METHO...
Autores principales: | , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Public Library of Science
2017
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5391941/ https://www.ncbi.nlm.nih.gov/pubmed/28410390 http://dx.doi.org/10.1371/journal.pone.0175552 |
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author | Gomes-Fernandes, Meissiner Laabei, Maisem Pagan, Natalia Hidalgo, Jessica Molinos, Sònia Villar Hernandez, Raquel Domínguez-Villanueva, Dídac Jenkins, A. Toby A. Lacoma, Alicia Prat, Cristina |
author_facet | Gomes-Fernandes, Meissiner Laabei, Maisem Pagan, Natalia Hidalgo, Jessica Molinos, Sònia Villar Hernandez, Raquel Domínguez-Villanueva, Dídac Jenkins, A. Toby A. Lacoma, Alicia Prat, Cristina |
author_sort | Gomes-Fernandes, Meissiner |
collection | PubMed |
description | OBJECTIVE: Characterization of Staphylococcus aureus clinical isolates derived from lower respiratory tract infections (LRTIs), and correlation between the functionality of the accessory gene regulator (Agr) and genotypic and phenotypic characteristics, clinical variables and clinical outcome. METHODS: S aureus isolates derived from LRTIs and control groups (nasal carriage and bacteraemia) were genotyped using StaphyType DNA microarray. Agr activity was evaluated using the CAMP synergistic haemolysis assay and the Vesicle Lysis Test (VLT). Discordant strains were analysed by quantitative reverse-transcriptase real-time PCR (qRT-PCR). RESULTS: Agr was functional in 79.7% and 84.5% of strains according to the CAMP and VLT assays respectively. Higher concordance with RNAIII expression measured by qRT-PCR was observed with the VLT assay (76.2%) compared with the CAMP assay (23.8%). No statistically significant differences were observed in Agr functionality between the study groups, nor the phenotypical/genotypical bacterial characteristics. No association between increased mortality/respiratory complications and Agr function was observed. CONCLUSIONS: Agr activity was high (82.2%) in isolates from LRTIs suggesting the importance of this global regulator in lower respiratory tract colonisation and infection. However, equally high Agr activity was observed in isolates derived from nasal carriage and bacteraemia, contradictory to previous observations. Agr functionality measured by the VLT assay was superior to CAMP assay. |
format | Online Article Text |
id | pubmed-5391941 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2017 |
publisher | Public Library of Science |
record_format | MEDLINE/PubMed |
spelling | pubmed-53919412017-05-03 Accessory gene regulator (Agr) functionality in Staphylococcus aureus derived from lower respiratory tract infections Gomes-Fernandes, Meissiner Laabei, Maisem Pagan, Natalia Hidalgo, Jessica Molinos, Sònia Villar Hernandez, Raquel Domínguez-Villanueva, Dídac Jenkins, A. Toby A. Lacoma, Alicia Prat, Cristina PLoS One Research Article OBJECTIVE: Characterization of Staphylococcus aureus clinical isolates derived from lower respiratory tract infections (LRTIs), and correlation between the functionality of the accessory gene regulator (Agr) and genotypic and phenotypic characteristics, clinical variables and clinical outcome. METHODS: S aureus isolates derived from LRTIs and control groups (nasal carriage and bacteraemia) were genotyped using StaphyType DNA microarray. Agr activity was evaluated using the CAMP synergistic haemolysis assay and the Vesicle Lysis Test (VLT). Discordant strains were analysed by quantitative reverse-transcriptase real-time PCR (qRT-PCR). RESULTS: Agr was functional in 79.7% and 84.5% of strains according to the CAMP and VLT assays respectively. Higher concordance with RNAIII expression measured by qRT-PCR was observed with the VLT assay (76.2%) compared with the CAMP assay (23.8%). No statistically significant differences were observed in Agr functionality between the study groups, nor the phenotypical/genotypical bacterial characteristics. No association between increased mortality/respiratory complications and Agr function was observed. CONCLUSIONS: Agr activity was high (82.2%) in isolates from LRTIs suggesting the importance of this global regulator in lower respiratory tract colonisation and infection. However, equally high Agr activity was observed in isolates derived from nasal carriage and bacteraemia, contradictory to previous observations. Agr functionality measured by the VLT assay was superior to CAMP assay. Public Library of Science 2017-04-14 /pmc/articles/PMC5391941/ /pubmed/28410390 http://dx.doi.org/10.1371/journal.pone.0175552 Text en © 2017 Gomes-Fernandes et al http://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. |
spellingShingle | Research Article Gomes-Fernandes, Meissiner Laabei, Maisem Pagan, Natalia Hidalgo, Jessica Molinos, Sònia Villar Hernandez, Raquel Domínguez-Villanueva, Dídac Jenkins, A. Toby A. Lacoma, Alicia Prat, Cristina Accessory gene regulator (Agr) functionality in Staphylococcus aureus derived from lower respiratory tract infections |
title | Accessory gene regulator (Agr) functionality in Staphylococcus aureus derived from lower respiratory tract infections |
title_full | Accessory gene regulator (Agr) functionality in Staphylococcus aureus derived from lower respiratory tract infections |
title_fullStr | Accessory gene regulator (Agr) functionality in Staphylococcus aureus derived from lower respiratory tract infections |
title_full_unstemmed | Accessory gene regulator (Agr) functionality in Staphylococcus aureus derived from lower respiratory tract infections |
title_short | Accessory gene regulator (Agr) functionality in Staphylococcus aureus derived from lower respiratory tract infections |
title_sort | accessory gene regulator (agr) functionality in staphylococcus aureus derived from lower respiratory tract infections |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5391941/ https://www.ncbi.nlm.nih.gov/pubmed/28410390 http://dx.doi.org/10.1371/journal.pone.0175552 |
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