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Accessory gene regulator (Agr) functionality in Staphylococcus aureus derived from lower respiratory tract infections

OBJECTIVE: Characterization of Staphylococcus aureus clinical isolates derived from lower respiratory tract infections (LRTIs), and correlation between the functionality of the accessory gene regulator (Agr) and genotypic and phenotypic characteristics, clinical variables and clinical outcome. METHO...

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Autores principales: Gomes-Fernandes, Meissiner, Laabei, Maisem, Pagan, Natalia, Hidalgo, Jessica, Molinos, Sònia, Villar Hernandez, Raquel, Domínguez-Villanueva, Dídac, Jenkins, A. Toby A., Lacoma, Alicia, Prat, Cristina
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5391941/
https://www.ncbi.nlm.nih.gov/pubmed/28410390
http://dx.doi.org/10.1371/journal.pone.0175552
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author Gomes-Fernandes, Meissiner
Laabei, Maisem
Pagan, Natalia
Hidalgo, Jessica
Molinos, Sònia
Villar Hernandez, Raquel
Domínguez-Villanueva, Dídac
Jenkins, A. Toby A.
Lacoma, Alicia
Prat, Cristina
author_facet Gomes-Fernandes, Meissiner
Laabei, Maisem
Pagan, Natalia
Hidalgo, Jessica
Molinos, Sònia
Villar Hernandez, Raquel
Domínguez-Villanueva, Dídac
Jenkins, A. Toby A.
Lacoma, Alicia
Prat, Cristina
author_sort Gomes-Fernandes, Meissiner
collection PubMed
description OBJECTIVE: Characterization of Staphylococcus aureus clinical isolates derived from lower respiratory tract infections (LRTIs), and correlation between the functionality of the accessory gene regulator (Agr) and genotypic and phenotypic characteristics, clinical variables and clinical outcome. METHODS: S aureus isolates derived from LRTIs and control groups (nasal carriage and bacteraemia) were genotyped using StaphyType DNA microarray. Agr activity was evaluated using the CAMP synergistic haemolysis assay and the Vesicle Lysis Test (VLT). Discordant strains were analysed by quantitative reverse-transcriptase real-time PCR (qRT-PCR). RESULTS: Agr was functional in 79.7% and 84.5% of strains according to the CAMP and VLT assays respectively. Higher concordance with RNAIII expression measured by qRT-PCR was observed with the VLT assay (76.2%) compared with the CAMP assay (23.8%). No statistically significant differences were observed in Agr functionality between the study groups, nor the phenotypical/genotypical bacterial characteristics. No association between increased mortality/respiratory complications and Agr function was observed. CONCLUSIONS: Agr activity was high (82.2%) in isolates from LRTIs suggesting the importance of this global regulator in lower respiratory tract colonisation and infection. However, equally high Agr activity was observed in isolates derived from nasal carriage and bacteraemia, contradictory to previous observations. Agr functionality measured by the VLT assay was superior to CAMP assay.
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spelling pubmed-53919412017-05-03 Accessory gene regulator (Agr) functionality in Staphylococcus aureus derived from lower respiratory tract infections Gomes-Fernandes, Meissiner Laabei, Maisem Pagan, Natalia Hidalgo, Jessica Molinos, Sònia Villar Hernandez, Raquel Domínguez-Villanueva, Dídac Jenkins, A. Toby A. Lacoma, Alicia Prat, Cristina PLoS One Research Article OBJECTIVE: Characterization of Staphylococcus aureus clinical isolates derived from lower respiratory tract infections (LRTIs), and correlation between the functionality of the accessory gene regulator (Agr) and genotypic and phenotypic characteristics, clinical variables and clinical outcome. METHODS: S aureus isolates derived from LRTIs and control groups (nasal carriage and bacteraemia) were genotyped using StaphyType DNA microarray. Agr activity was evaluated using the CAMP synergistic haemolysis assay and the Vesicle Lysis Test (VLT). Discordant strains were analysed by quantitative reverse-transcriptase real-time PCR (qRT-PCR). RESULTS: Agr was functional in 79.7% and 84.5% of strains according to the CAMP and VLT assays respectively. Higher concordance with RNAIII expression measured by qRT-PCR was observed with the VLT assay (76.2%) compared with the CAMP assay (23.8%). No statistically significant differences were observed in Agr functionality between the study groups, nor the phenotypical/genotypical bacterial characteristics. No association between increased mortality/respiratory complications and Agr function was observed. CONCLUSIONS: Agr activity was high (82.2%) in isolates from LRTIs suggesting the importance of this global regulator in lower respiratory tract colonisation and infection. However, equally high Agr activity was observed in isolates derived from nasal carriage and bacteraemia, contradictory to previous observations. Agr functionality measured by the VLT assay was superior to CAMP assay. Public Library of Science 2017-04-14 /pmc/articles/PMC5391941/ /pubmed/28410390 http://dx.doi.org/10.1371/journal.pone.0175552 Text en © 2017 Gomes-Fernandes et al http://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
spellingShingle Research Article
Gomes-Fernandes, Meissiner
Laabei, Maisem
Pagan, Natalia
Hidalgo, Jessica
Molinos, Sònia
Villar Hernandez, Raquel
Domínguez-Villanueva, Dídac
Jenkins, A. Toby A.
Lacoma, Alicia
Prat, Cristina
Accessory gene regulator (Agr) functionality in Staphylococcus aureus derived from lower respiratory tract infections
title Accessory gene regulator (Agr) functionality in Staphylococcus aureus derived from lower respiratory tract infections
title_full Accessory gene regulator (Agr) functionality in Staphylococcus aureus derived from lower respiratory tract infections
title_fullStr Accessory gene regulator (Agr) functionality in Staphylococcus aureus derived from lower respiratory tract infections
title_full_unstemmed Accessory gene regulator (Agr) functionality in Staphylococcus aureus derived from lower respiratory tract infections
title_short Accessory gene regulator (Agr) functionality in Staphylococcus aureus derived from lower respiratory tract infections
title_sort accessory gene regulator (agr) functionality in staphylococcus aureus derived from lower respiratory tract infections
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5391941/
https://www.ncbi.nlm.nih.gov/pubmed/28410390
http://dx.doi.org/10.1371/journal.pone.0175552
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