DNA-free RNA preparations from mycobacteria

BACKGROUND: To understand mycobacterial pathogenesis analysis of gene expression by quantification of RNA levels becomes increasingly important. However, current preparation methods yield mycobacterial RNA that is contaminated with chromosomal DNA. RESULTS: After sonication of RNA samples from Mycob...

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Detalles Bibliográficos
Autores principales: Stephan, Joachim, Bail, Johannes G, Titgemeyer, Fritz, Niederweis, Michael
Formato: Texto
Lenguaje:English
Publicado: BioMed Central 2004
Materias:
Methodology Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC539233/
https://www.ncbi.nlm.nih.gov/pubmed/15571628
http://dx.doi.org/10.1186/1471-2180-4-45
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author Stephan, Joachim
Bail, Johannes G
Titgemeyer, Fritz
Niederweis, Michael
author_facet Stephan, Joachim
Bail, Johannes G
Titgemeyer, Fritz
Niederweis, Michael
author_sort Stephan, Joachim
collection PubMed
description BACKGROUND: To understand mycobacterial pathogenesis analysis of gene expression by quantification of RNA levels becomes increasingly important. However, current preparation methods yield mycobacterial RNA that is contaminated with chromosomal DNA. RESULTS: After sonication of RNA samples from Mycobacterium smegmatis genomic DNA is efficiently removed by DNaseI in contrast to untreated samples. CONCLUSIONS: This procedure eliminates one of the most prevalent error sources in quantification of RNA levels in mycobacteria.
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spelling pubmed-5392332004-12-24 DNA-free RNA preparations from mycobacteria Stephan, Joachim Bail, Johannes G Titgemeyer, Fritz Niederweis, Michael BMC Microbiol Methodology Article BACKGROUND: To understand mycobacterial pathogenesis analysis of gene expression by quantification of RNA levels becomes increasingly important. However, current preparation methods yield mycobacterial RNA that is contaminated with chromosomal DNA. RESULTS: After sonication of RNA samples from Mycobacterium smegmatis genomic DNA is efficiently removed by DNaseI in contrast to untreated samples. CONCLUSIONS: This procedure eliminates one of the most prevalent error sources in quantification of RNA levels in mycobacteria. BioMed Central 2004-11-30 /pmc/articles/PMC539233/ /pubmed/15571628 http://dx.doi.org/10.1186/1471-2180-4-45 Text en Copyright © 2004 Stephan et al; licensee BioMed Central Ltd.
spellingShingle Methodology Article
Stephan, Joachim
Bail, Johannes G
Titgemeyer, Fritz
Niederweis, Michael
DNA-free RNA preparations from mycobacteria
title DNA-free RNA preparations from mycobacteria
title_full DNA-free RNA preparations from mycobacteria
title_fullStr DNA-free RNA preparations from mycobacteria
title_full_unstemmed DNA-free RNA preparations from mycobacteria
title_short DNA-free RNA preparations from mycobacteria
title_sort dna-free rna preparations from mycobacteria
topic Methodology Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC539233/
https://www.ncbi.nlm.nih.gov/pubmed/15571628
http://dx.doi.org/10.1186/1471-2180-4-45
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AT bailjohannesg dnafreernapreparationsfrommycobacteria
AT titgemeyerfritz dnafreernapreparationsfrommycobacteria
AT niederweismichael dnafreernapreparationsfrommycobacteria

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