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Functional effects of 17alpha-hydroxyprogesterone caproate (17P) on human myometrial contractility in vitro
BACKGROUND: 17alpha-hydroxyprogesterone caproate (17P) administration reportedly improves outcome for women with a previous spontaneous preterm delivery. This study, using in vitro strips of human uterine smooth muscle, aimed to investigate the direct non-genomic effects of 17P on spontaneous and in...
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Formato: | Texto |
Lenguaje: | English |
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BioMed Central
2004
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Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC539291/ https://www.ncbi.nlm.nih.gov/pubmed/15585068 http://dx.doi.org/10.1186/1477-7827-2-80 |
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author | Sexton, Donal J O'Reilly, Michael W Friel, Anne M Morrison, John J |
author_facet | Sexton, Donal J O'Reilly, Michael W Friel, Anne M Morrison, John J |
author_sort | Sexton, Donal J |
collection | PubMed |
description | BACKGROUND: 17alpha-hydroxyprogesterone caproate (17P) administration reportedly improves outcome for women with a previous spontaneous preterm delivery. This study, using in vitro strips of human uterine smooth muscle, aimed to investigate the direct non-genomic effects of 17P on spontaneous and induced contractions in tissues obtained during pregnancy, and in the non-pregnant state. METHODS: Biopsies of human myometrium were obtained at elective cesarean section, and from hysterectomy specimens, and dissected strips suspended for isometric recordings. The effects of 17P (1 nmol/L -10 micro mol/L) on spontaneous and agonist-induced (oxytocin 0.5 nmol/L for pregnant, phenylephrine 10 μmol/L for non-pregnant) contractions were measured. Integrals of contractile activity, including the mean maximal inhibition values (MMI) observed at the maximal concentration, were compared with those from simultaneously run control strips. RESULTS: There was no significant direct effect exerted by 17P on pregnant or non-pregnant human myometrial contractility. The MMI ± SEM for spontaneous contractions in pregnant myometrium was 4.9% ± 7.2 (n = 6; P = 0.309) and for oxytocin-induced contractions was 2.2% ± 1.3 (n = 6; P = 0.128). For non-pregnant myometrium, the MMI ± SEM for spontaneous contractions was 8.8% ± 11.0 (n = 6; P = 0.121) and for phenylephrine induced contractions was -7.9% ± 6.5 (n = 6; P = 0.966). CONCLUSIONS: The putative benefits of 17P for preterm labor prevention are not achieved, even partially, by a direct utero-relaxant effect. These findings outline the possibility that genomic effects of 17P, achieved over long periods of administration, are required for its reported therapeutic benefits. |
format | Text |
id | pubmed-539291 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2004 |
publisher | BioMed Central |
record_format | MEDLINE/PubMed |
spelling | pubmed-5392912004-12-26 Functional effects of 17alpha-hydroxyprogesterone caproate (17P) on human myometrial contractility in vitro Sexton, Donal J O'Reilly, Michael W Friel, Anne M Morrison, John J Reprod Biol Endocrinol Research BACKGROUND: 17alpha-hydroxyprogesterone caproate (17P) administration reportedly improves outcome for women with a previous spontaneous preterm delivery. This study, using in vitro strips of human uterine smooth muscle, aimed to investigate the direct non-genomic effects of 17P on spontaneous and induced contractions in tissues obtained during pregnancy, and in the non-pregnant state. METHODS: Biopsies of human myometrium were obtained at elective cesarean section, and from hysterectomy specimens, and dissected strips suspended for isometric recordings. The effects of 17P (1 nmol/L -10 micro mol/L) on spontaneous and agonist-induced (oxytocin 0.5 nmol/L for pregnant, phenylephrine 10 μmol/L for non-pregnant) contractions were measured. Integrals of contractile activity, including the mean maximal inhibition values (MMI) observed at the maximal concentration, were compared with those from simultaneously run control strips. RESULTS: There was no significant direct effect exerted by 17P on pregnant or non-pregnant human myometrial contractility. The MMI ± SEM for spontaneous contractions in pregnant myometrium was 4.9% ± 7.2 (n = 6; P = 0.309) and for oxytocin-induced contractions was 2.2% ± 1.3 (n = 6; P = 0.128). For non-pregnant myometrium, the MMI ± SEM for spontaneous contractions was 8.8% ± 11.0 (n = 6; P = 0.121) and for phenylephrine induced contractions was -7.9% ± 6.5 (n = 6; P = 0.966). CONCLUSIONS: The putative benefits of 17P for preterm labor prevention are not achieved, even partially, by a direct utero-relaxant effect. These findings outline the possibility that genomic effects of 17P, achieved over long periods of administration, are required for its reported therapeutic benefits. BioMed Central 2004-12-07 /pmc/articles/PMC539291/ /pubmed/15585068 http://dx.doi.org/10.1186/1477-7827-2-80 Text en Copyright © 2004 Sexton et al; licensee BioMed Central Ltd. http://creativecommons.org/licenses/by/2.0 This is an Open Access article distributed under the terms of the Creative Commons Attribution License ( (http://creativecommons.org/licenses/by/2.0) ), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Research Sexton, Donal J O'Reilly, Michael W Friel, Anne M Morrison, John J Functional effects of 17alpha-hydroxyprogesterone caproate (17P) on human myometrial contractility in vitro |
title | Functional effects of 17alpha-hydroxyprogesterone caproate (17P) on human myometrial contractility in vitro |
title_full | Functional effects of 17alpha-hydroxyprogesterone caproate (17P) on human myometrial contractility in vitro |
title_fullStr | Functional effects of 17alpha-hydroxyprogesterone caproate (17P) on human myometrial contractility in vitro |
title_full_unstemmed | Functional effects of 17alpha-hydroxyprogesterone caproate (17P) on human myometrial contractility in vitro |
title_short | Functional effects of 17alpha-hydroxyprogesterone caproate (17P) on human myometrial contractility in vitro |
title_sort | functional effects of 17alpha-hydroxyprogesterone caproate (17p) on human myometrial contractility in vitro |
topic | Research |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC539291/ https://www.ncbi.nlm.nih.gov/pubmed/15585068 http://dx.doi.org/10.1186/1477-7827-2-80 |
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