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Optogenetic perturbation and bioluminescence imaging to analyze cell-to-cell transfer of oscillatory information
Cells communicate with each other to coordinate their gene activities at the population level through signaling pathways. It has been shown that many gene activities are oscillatory and that the frequency and phase of oscillatory gene expression encode various types of information. However, whether...
Autores principales: | , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Cold Spring Harbor Laboratory Press
2017
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5393066/ https://www.ncbi.nlm.nih.gov/pubmed/28373207 http://dx.doi.org/10.1101/gad.294546.116 |
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author | Isomura, Akihiro Ogushi, Fumiko Kori, Hiroshi Kageyama, Ryoichiro |
author_facet | Isomura, Akihiro Ogushi, Fumiko Kori, Hiroshi Kageyama, Ryoichiro |
author_sort | Isomura, Akihiro |
collection | PubMed |
description | Cells communicate with each other to coordinate their gene activities at the population level through signaling pathways. It has been shown that many gene activities are oscillatory and that the frequency and phase of oscillatory gene expression encode various types of information. However, whether or how such oscillatory information is transmitted from cell to cell remains unknown. Here, we developed an integrated approach that combines optogenetic perturbations and single-cell bioluminescence imaging to visualize and reconstitute synchronized oscillatory gene expression in signal-sending and signal-receiving processes. We found that intracellular and intercellular periodic inputs of Notch signaling entrain intrinsic oscillations by frequency tuning and phase shifting at the single-cell level. In this way, the oscillation dynamics are transmitted through Notch signaling, thereby synchronizing the population of oscillators. Thus, this approach enabled us to control and monitor dynamic cell-to-cell transfer of oscillatory information to coordinate gene expression patterns at the population level. |
format | Online Article Text |
id | pubmed-5393066 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2017 |
publisher | Cold Spring Harbor Laboratory Press |
record_format | MEDLINE/PubMed |
spelling | pubmed-53930662017-04-25 Optogenetic perturbation and bioluminescence imaging to analyze cell-to-cell transfer of oscillatory information Isomura, Akihiro Ogushi, Fumiko Kori, Hiroshi Kageyama, Ryoichiro Genes Dev Resource/Methodology Cells communicate with each other to coordinate their gene activities at the population level through signaling pathways. It has been shown that many gene activities are oscillatory and that the frequency and phase of oscillatory gene expression encode various types of information. However, whether or how such oscillatory information is transmitted from cell to cell remains unknown. Here, we developed an integrated approach that combines optogenetic perturbations and single-cell bioluminescence imaging to visualize and reconstitute synchronized oscillatory gene expression in signal-sending and signal-receiving processes. We found that intracellular and intercellular periodic inputs of Notch signaling entrain intrinsic oscillations by frequency tuning and phase shifting at the single-cell level. In this way, the oscillation dynamics are transmitted through Notch signaling, thereby synchronizing the population of oscillators. Thus, this approach enabled us to control and monitor dynamic cell-to-cell transfer of oscillatory information to coordinate gene expression patterns at the population level. Cold Spring Harbor Laboratory Press 2017-03-01 /pmc/articles/PMC5393066/ /pubmed/28373207 http://dx.doi.org/10.1101/gad.294546.116 Text en © 2017 Isomura et al.; Published by Cold Spring Harbor Laboratory Press http://creativecommons.org/licenses/by/4.0/ This article, published in Genes & Development, is available under a Creative Commons License (Attribution 4.0 International), as described at http://creativecommons.org/licenses/by/4.0/. |
spellingShingle | Resource/Methodology Isomura, Akihiro Ogushi, Fumiko Kori, Hiroshi Kageyama, Ryoichiro Optogenetic perturbation and bioluminescence imaging to analyze cell-to-cell transfer of oscillatory information |
title | Optogenetic perturbation and bioluminescence imaging to analyze cell-to-cell transfer of oscillatory information |
title_full | Optogenetic perturbation and bioluminescence imaging to analyze cell-to-cell transfer of oscillatory information |
title_fullStr | Optogenetic perturbation and bioluminescence imaging to analyze cell-to-cell transfer of oscillatory information |
title_full_unstemmed | Optogenetic perturbation and bioluminescence imaging to analyze cell-to-cell transfer of oscillatory information |
title_short | Optogenetic perturbation and bioluminescence imaging to analyze cell-to-cell transfer of oscillatory information |
title_sort | optogenetic perturbation and bioluminescence imaging to analyze cell-to-cell transfer of oscillatory information |
topic | Resource/Methodology |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5393066/ https://www.ncbi.nlm.nih.gov/pubmed/28373207 http://dx.doi.org/10.1101/gad.294546.116 |
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