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Gene expression control by Bacillus anthracis purine riboswitches

In all kingdoms of life, cellular replication relies on the presence of nucleosides and nucleotides, the building blocks of nucleic acids and the main source of energy. In bacteria, the availability of metabolites sometimes directly regulates the expression of enzymes and proteins involved in purine...

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Detalles Bibliográficos
Autores principales: Kirchner, Marion, Schneider, Sabine
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Cold Spring Harbor Laboratory Press 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5393184/
https://www.ncbi.nlm.nih.gov/pubmed/28209633
http://dx.doi.org/10.1261/rna.058792.116
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author Kirchner, Marion
Schneider, Sabine
author_facet Kirchner, Marion
Schneider, Sabine
author_sort Kirchner, Marion
collection PubMed
description In all kingdoms of life, cellular replication relies on the presence of nucleosides and nucleotides, the building blocks of nucleic acids and the main source of energy. In bacteria, the availability of metabolites sometimes directly regulates the expression of enzymes and proteins involved in purine salvage, biosynthesis, and uptake through riboswitches. Riboswitches are located in bacterial mRNAs and can control gene expression by conformational changes in response to ligand binding. We have established an inverse reporter gene system in Bacillus subtilis that allows us to monitor riboswitch-controlled gene expression. We used it to investigate the activity of five potential purine riboswitches from Bacillus anthracis in response to different purines and pyrimidines. Furthermore, in vitro studies on the aptamer domains of the riboswitches reveal their variation in guanine binding affinity ranging from namomolar to micromolar. These data do not only provide insight into metabolite sensing but can also aid in engineering artificial cell regulatory systems.
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spelling pubmed-53931842018-05-01 Gene expression control by Bacillus anthracis purine riboswitches Kirchner, Marion Schneider, Sabine RNA Article In all kingdoms of life, cellular replication relies on the presence of nucleosides and nucleotides, the building blocks of nucleic acids and the main source of energy. In bacteria, the availability of metabolites sometimes directly regulates the expression of enzymes and proteins involved in purine salvage, biosynthesis, and uptake through riboswitches. Riboswitches are located in bacterial mRNAs and can control gene expression by conformational changes in response to ligand binding. We have established an inverse reporter gene system in Bacillus subtilis that allows us to monitor riboswitch-controlled gene expression. We used it to investigate the activity of five potential purine riboswitches from Bacillus anthracis in response to different purines and pyrimidines. Furthermore, in vitro studies on the aptamer domains of the riboswitches reveal their variation in guanine binding affinity ranging from namomolar to micromolar. These data do not only provide insight into metabolite sensing but can also aid in engineering artificial cell regulatory systems. Cold Spring Harbor Laboratory Press 2017-05 /pmc/articles/PMC5393184/ /pubmed/28209633 http://dx.doi.org/10.1261/rna.058792.116 Text en © 2017 Kirchner and Schneider; Published by Cold Spring Harbor Laboratory Press for the RNA Society http://creativecommons.org/licenses/by-nc/4.0/ This article is distributed exclusively by the RNA Society for the first 12 months after the full-issue publication date (see http://rnajournal.cshlp.org/site/misc/terms.xhtml). After 12 months, it is available under a Creative Commons License (Attribution-NonCommercial 4.0 International), as described at http://creativecommons.org/licenses/by-nc/4.0/.
spellingShingle Article
Kirchner, Marion
Schneider, Sabine
Gene expression control by Bacillus anthracis purine riboswitches
title Gene expression control by Bacillus anthracis purine riboswitches
title_full Gene expression control by Bacillus anthracis purine riboswitches
title_fullStr Gene expression control by Bacillus anthracis purine riboswitches
title_full_unstemmed Gene expression control by Bacillus anthracis purine riboswitches
title_short Gene expression control by Bacillus anthracis purine riboswitches
title_sort gene expression control by bacillus anthracis purine riboswitches
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5393184/
https://www.ncbi.nlm.nih.gov/pubmed/28209633
http://dx.doi.org/10.1261/rna.058792.116
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